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Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli
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Leader sequences have a length of approximately 500 bp, stand upstream of CRISPR loci, and carry promoter elements and signals for CRISPR systems adaptation that are crucial to crRNA transcription and the successful integration of foreign genetic material into CRISPR sequences.
“These leader sequences have a length of approximately 500 bp and carry promoter elements and signals for CRISPR systems adaptation that are crucial to the transcription of crRNA (CRISPR RNA) and the successful integration of foreign genetic material into CRISPR sequences [11,12].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [12]
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Experimental — accuracy not yet measuredBoth Cas1 and Cas2 are required for adaptation because expression of Cas1 or Cas2 on their own does not potentiate spacer acquisition.
“Both proteins are required for this step since the expression of Cas1 or Cas2 on their own does not potentiate spacer acquisition [12].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [12]
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
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Research works printed but not linked: 26
- Marraffini, 2008, CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA, Science, 322, 1843, 10.1126/science.1165771 · DOI · Paper page
- Barrangou, 2007, CRISPR provides acquired resistance against viruses in prokaryotes, Science, 315, 1709, 10.1126/science.1138140 · DOI · Paper page
- Brouns, 2008, Small CRISPR RNAs guide antiviral defense in prokaryotes, Science, 321, 960, 10.1126/science.1159689 · DOI · Paper page
- Hale, 2009, RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex, Cell, 139, 945, 10.1016/j.cell.2009.07.040 · DOI · Paper page
- Deveau, 2010, CRISPR/Cas system and its role in phage-bacteria interactions, Annu. Rev. Microbiol., 64, 475, 10.1146/annurev.micro.112408.134123 · DOI
- Sorek, 2008, CRISPR–a widespread system that provides acquired resistance against phages in bacteria and archaea, Nat. Rev. Microbiol., 6, 181, 10.1038/nrmicro1793 · DOI
- Marraffini, 2010, CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea, Nat. Rev. Genet., 11, 181, 10.1038/nrg2749 · DOI · Paper page
- Makarova, 2011, Evolution and classification of the CRISPR-Cas systems, Nat. Rev. Microbiol., 9, 467, 10.1038/nrmicro2577 · DOI · Paper page
- van der Ploeg, 2009, Analysis of CRISPR in Streptococcus mutans suggests frequent occurrence of acquired immunity against infection by M102-like bacteriophages, Microbiology, 155, 1966, 10.1099/mic.0.027508-0 · DOI
- Bhaya, 2011, CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation, Annu. Rev. Genet., 45, 273, 10.1146/annurev-genet-110410-132430 · DOI · Paper page
- Wiedenheft, 2009, Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense, Structure, 17, 904, 10.1016/j.str.2009.03.019 · DOI · Paper page
- Babu, 2011, A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair, Mol. Microbiol., 79, 484, 10.1111/j.1365-2958.2010.07465.x · DOI · Paper page
- Beloglazova, 2008, A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats, J. Biol. Chem., 283, 20361, 10.1074/jbc.M803225200 · DOI
- Deveau, 2008, Phage response to CRISPR-encoded resistance in Streptococcus thermophilus, J. Bacteriol., 190, 1390, 10.1128/JB.01412-07 · DOI
- Bolotin, 2005, Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin, Microbiology, 151, 2551, 10.1099/mic.0.28048-0 · DOI · Paper page
- Mojica, 2009, Short motif sequences determine the targets of the prokaryotic CRISPR defence system, Microbiology, 155, 733, 10.1099/mic.0.023960-0 · DOI · Paper page
- Díez-Villaseñor, 2010, Diversity of CRISPR loci in Escherichia coli, Microbiology, 156, 1351, 10.1099/mic.0.036046-0 · DOI
- Pougach, 2010, Transcription, processing and function of CRISPR cassettes in Escherichia coli, Mol. Microbiol., 77, 1367, 10.1111/j.1365-2958.2010.07265.x · DOI
- Yosef, 2011, High-temperature protein G is essential for activity of the Escherichia coli clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, Proc. Natl Acad. Sci. USA, 108, 20136, 10.1073/pnas.1113519108 · DOI
- Pul, 2010, Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS, Mol. Microbiol., 75, 1495, 10.1111/j.1365-2958.2010.07073.x · DOI · Paper page
- Kool, 1974, Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13, J. Bacteriol., 120, 569, 10.1128/JB.120.2.569-578.1974 · DOI
- Crooks, 2004, WebLogo: a sequence logo generator, Genome Res., 14, 1188, 10.1101/gr.849004 · DOI
- Gudbergsdottir, 2011, Dynamic properties of the sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacers, Mol. Microbiol., 79, 35, 10.1111/j.1365-2958.2010.07452.x · DOI
- Sharan, 2009, Recombineering: a homologous recombination-based method of genetic engineering, Nat. Protoc., 4, 206, 10.1038/nprot.2008.227 · DOI
- Datta, 2006, A set of recombineering plasmids for gram-negative bacteria, Gene, 379, 109, 10.1016/j.gene.2006.04.018 · DOI
- Datsenko, 2000, One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, Proc. Natl Acad. Sci. USA, 97, 6640, 10.1073/pnas.120163297 · DOI
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Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Konermann, S., Lotfy, P., Brideau, N. J., Oki, J., Shokhirev, M. N., & Hsu, P. D. (2018) · Cell · doi:10.1016/j.cell.2018.02.033 · Cited by 1,164
CRISPR-mediated adaptive immune systems in bacteria and archaea
Sorek, R., Lawrence, C. M., & Wiedenheft, B. (2013) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-072911-172315 · Cited by 668
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications
Liu, C., Zhang, L., Liu, H., & Cheng, K. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.09.012 · Cited by 607
Ishino, Y., Krupovic, M., & Forterre, P. (2018) · Journal of Bacteriology · doi:10.1128/jb.00580-17 · Cited by 558
Classification and evolution of type II CRISPR-Cas systems
Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku241 · Cited by 498
CRISPR-Cas Encoding of a Digital Movie into the Genomes of a Population of Living Bacteria
Shipman, S. L., Nivala, J., Macklis, J. D., & Church, G. M. (2017) · Nature · doi:10.1038/nature23017 · Cited by 361
Loureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
RNA-Targeting CRISPR-Cas systems and their applications
Burmistrz, M., Krakowski, K., & Krawczyk-Balska, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031122 · Cited by 125
Characterization and Repurposing of Type I and Type II CRISPR–Cas Systems in Bacteria
Hidalgo-Cantabrana, C., Goh, Y. J., & Barrangou, R. (2019) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.09.013 · Cited by 63
PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation
Dhingra, Y., Suresh, S. K., Juneja, P., & Sashital, D. G. (2022) · Molecular Cell · doi:10.1016/j.molcel.2022.09.030 · Cited by 21
Metadata
- Authors
- Yosef, I., Goren, M. G., & Qimron, U.
- Affiliations
- Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-02-08
- Volume
- 40
- Issue
- 12
- Pages
- 5569-5576
- ISSN
- 1362-4962
- 0305-1048
- PMC
- PMC3384332
- Cited by
- 655
- References
- 1
- Language
- en
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