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Cited by 168

The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.

Single-molecule analysis of transcription factor binding at transcription sites in live cells

Open access
Morisaki, Tatsuya, Müller, Waltraud G., Golob, Nicole, Mazza, Davide, McNally, James G.·2014·Nature Communications
DOI 10.1038/ncomms5456
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Abstract

Although numerous live-cell measurements have shown that transcription factors (TFs) bind chromatin transiently, no measurements of transient binding have been reported at the endogenous response elements (REs) where transcription is normally induced. Here we show that at endogenous REs the transcriptionally productive specific binding of two TFs, p53 and the glucocorticoid receptor (GR), is transient. We also find that the transient residence times of GR at endogenous REs are roughly comparable to those at an artificial, multi-copy array of gene regulatory sites, supporting the use of multi-copy arrays for live-cell analysis of transcription. Finally, we find that at any moment only a small fraction of TF molecules are engaged in transcriptionally productive binding at endogenous REs. The small fraction of bound factors provides one explanation for gene bursting and it also indicates that REs may often be unoccupied, resulting in partial responses to transcriptional signals. Despite numerous previous studies, the nature of the interaction of transcription factors (TF) with their endogenous response elements (REs) has remained unclear. Here the authors characterize the binding of p53 and glucocorticoid receptor to their endogenous REs and find that transcriptionally productive interactions are transient and involve only a small fraction of cellular TF molecules.

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Citations

Cites

Research works printed but not linked: 23

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Metadata

Authors
Morisaki, T., Müller, W. G., Golob, N., Mazza, D., & McNally, J. G.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2014-07-18
Volume
5
Issue
1
Pages
4456-4456
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms5456
OpenAlex
W1980586499
Cited by
168
References
10
FWCI
7.42
Type
journal article
Language
en
Open access
bronze
Licence

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