Transcripts Targeted by the MicroRNA-16 Family Cooperatively Regulate Cell Cycle Progression
Open accessAbstract
microRNAs (miRNAs) are abundant, approximately 21-nucleotide, noncoding regulatory RNAs. Each miRNA may regulate hundreds of mRNA targets, but the identities of these targets and the processes they regulate are poorly understood. Here we have explored the use of microarray profiling and functional screening to identify targets and biological processes triggered by the transfection of human cells with miRNAs. We demonstrate that a family of miRNAs sharing sequence identity with miRNA-16 (miR-16) negatively regulates cellular growth and cell cycle progression. miR-16-down-regulated transcripts were enriched with genes whose silencing by small interfering RNAs causes an accumulation of cells in G(0)/G(1). Simultaneous silencing of these genes was more effective at blocking cell cycle progression than disruption of the individual genes. Thus, miR-16 coordinately regulates targets that may act in concert to control cell cycle progression.
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Members of the miR-15/107 family have overlapping mRNA targets.
“These miRNAs have overlapping targets.”
From Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells (2014) · cites this paper as [41]
Since miR-15/107 miRNAs have highly overlapping targets and multiple members are transcribed at constitutively high levels, any therapeutic or diagnostic method based on one family member must also take into account the biological activities of other family members.
“In other words, since miR-15/107 miRNAs have highly overlapping targets [7,16,41], and multiple members of the gene family are transcribed at constitutively high levels, any therapeutic or diagnostic method based on one family member must also take into account the biological activities of other family members.”
From Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells (2014) · cites this paper as [41]
The miR-16 family of miRNAs regulates the G0 to G1 transition.
“It has previously been shown that the miR-16 family of miRNAs has a role in regulating G0 to G1 transition139.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [139]
Silencing transcripts that miR-16 downregulates also affects cell cycle progression.
“Intriguingly, silencing of transcripts that are downregulated by miR-16 also affects cell cycle progression139.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [139]
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- Authors
- Linsley, P. S., Schelter, J., Burchard, J., Kibukawa, M., Martin, M. M., Bartz, S. R., Johnson, J. M., Cummins, J. M., Raymond, C. K., Dai, H., Chau, N., Cleary, M., Jackson, A. L., Carleton, M., & Lim, L.
- Affiliations
- Rosetta Inpharmatics, LLC
- Howard Hughes Medical Institute (US)
- ORCID
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2007-03-01
- Volume
- 27
- Issue
- 6
- Pages
- 2240-2252
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC1820501
- OpenAlex
- W2128989640
- Cited by
- 552
- References
- 54
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 6 · 2023: 17 · 2022: 10 · 2021: 17 · 2020: 17 · 2019: 19 · 2018: 16 · 2017: 21 · 2016: 23 · 2015: 32 · 2014: 40 · 2013: 48 · 2012: 55
- FWCI
- 20.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- miR-16, miRNA target identification, cell cycle progression, G0/G1 cell cycle arrest, gene silencing, siRNA, miRNA families
- MeSH terms
- Cell Cycle, Cell Line, Multigene Family, Humans, Neoplasms, Phenotype, Transcription, Genetic, Down-Regulation, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs
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