The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling
Open accessAbstract
Neural progenitor cell (NPC) culture within 3D hydrogels is an attractive strategy for expanding a therapeutically-relevant number of stem cells. However, relatively little is known about how 3D material properties such as stiffness and degradability affect the maintenance of NPC stemness in the absence of differentiation factors. Over a physiologically-relevant range of stiffness from ~0.5–50 kPa, stemness maintenance did not correlate with initial hydrogel stiffness. In contrast, hydrogel degradation was both correlated with, and necessary for, maintenance of NPC stemness. This requirement for degradation was independent of cytoskeletal tension generation and presentation of engineered adhesive ligands, instead relying on matrix remodeling to facilitate cadherin-mediated cell-cell contact and promote β-catenin signaling. In two additional hydrogel systems, permitting NPC-mediated matrix remodeling proved to be a generalizable strategy for stemness maintenance in 3D. Our findings have identified matrix remodeling, in the absence of cytoskeletal tension generation, as a previously unknown strategy to maintain stemness in 3D.
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Cites
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Self-citationHydrogel scaffolds for tissue engineering: the importance of polymer choice
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Bioengineering Approaches for the Advanced Organoid Research
Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K. (2021) · Advanced Materials · doi:10.1002/adma.202007949 · Cited by 218
Design of self-assembly dipeptide hydrogels and machine learning via their chemical features
Li, F., Han, J., Cao, T., Lam, W., Fan, B., Tang, W., Chen, S., Fok, K. L., & Li, L. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1903376116 · Cited by 198
Metadata
- Authors
- Madl, C. M., LeSavage, B. L., Dewi, R. E., Dinh, C. B., Stowers, R. S., Khariton, M., Lampe, K. J., Nguyen, D., Chaudhuri, O., Enejder, A., & Heilshorn, S. C.
- Venue
- Nature Materials
- Published
- 2017-10-30
- Volume
- 16
- Issue
- 12
- Pages
- 1233-1242
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2765609903
- Cited by
- 384
- References
- 60
- FWCI
- 12.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Madl, C. M., LeSavage, B. L., Dewi, R. E., Dinh, C. B., Stowers, R. S., Khariton, M., Lampe, K. J., Nguyen, D., Chaudhuri, O., Enejder, A., & Heilshorn, S. C. (2017). Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling. Nature Materials. https://doi.org/10.1038/nmat5020
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