The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
Open accessAbstract
We demonstrate three-dimensional (3D) super-resolution in live multicellular organisms using structured illumination microscopy (SIM). Sparse multifocal illumination patterns generated by a digital micromirror device (DMD) allowed us to physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples eightfold thicker than had been previously imaged with SIM. We imaged samples at one 2D image per second, at resolutions as low as 145 nm laterally and 400 nm axially. In addition to dual-labeled, whole fixed cells, we imaged GFP-labeled microtubules in live transgenic zebrafish embryos at depths >45 μm. We captured dynamic changes in the zebrafish lateral line primordium and observed interactions between myosin IIA and F-actin in cells encapsulated in collagen gels, obtaining two-color 4D super-resolution data sets spanning tens of time points and minutes without apparent phototoxicity. Our method uses commercially available parts and open-source software and is simpler than existing SIM implementations, allowing easy integration with wide-field microscopes.
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Cites
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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
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Python for scientific computing
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Cited by
Super-Resolution Structured Illumination Microscopy
Heintzmann, R., & Huser, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00218 · Cited by 574
Faster, sharper, and deeper: structured illumination microscopy for biological imaging
Wu, Y., & Shroff, H. (2018) · Nature Methods · doi:10.1038/s41592-018-0211-z · Cited by 375
Self-citationNoninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens
Gao, L., Shao, L., Higgins, C. D., Poulton, J. S., Peifer, M., Davidson, M. W., Wu, X., Goldstein, B., & Betzig, E. (2012) · Cell · doi:10.1016/j.cell.2012.10.008 · Cited by 320
Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex
Naito, T., Ercan, B., Krshnan, L., Triebl, A., Koh, D. H. Z., Wei, F.-Y., Tomizawa, K., Torta, F. T., Wenk, M. R., & Saheki, Y. (2019) · eLife · doi:10.7554/elife.51401 · Cited by 171
Live-cell imaging powered by computation
Shroff, H., Testa, I., Jug, F., & Manley, S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00702-6 · Cited by 75
Self-citation
Metadata
- Authors
- York, A. G., Parekh, S. H., Nogare, D. D., Fischer, R. S., Temprine, K., Mione, M., Chitnis, A. B., Combs, C. A., & Shroff, H.
- Venue
- Nature Methods
- Published
- 2012-05-13
- Volume
- 9
- Issue
- 7
- Pages
- 749-754
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2021248782
- Cited by
- 472
- References
- 36
- FWCI
- 12.79
- Type
- journal article
- Language
- en
- Open access
- green
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