The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
A culture system to study oligodendrocyte myelination processes using engineered nanofibers
Open accessAbstract
Current methods for studying central nervous system myelination necessitate permissive axonal substrates conducive to myelin wrapping by oligodendrocytes. We have developed a neuron-free culture system in which electron-spun nanofibers of varying sizes substitute for axons as a substrate for oligodendrocyte myelination, thereby allowing manipulation of the biophysical elements of axonal-oligodendroglial interactions. To investigate axonal regulation of myelination, this system effectively uncouples the role of molecular (inductive) cues from that of biophysical properties of the axon. We use this method to uncover the causation and sufficiency of fiber diameter in the initiation of concentric wrapping by rat oligodendrocytes. We also show that oligodendrocyte precursor cells display sensitivity to the biophysical properties of fiber diameter and initiate membrane ensheathment before differentiation. The use of nanofiber scaffolds will enable screening for potential therapeutic agents that promote oligodendrocyte differentiation and myelination and will also provide valuable insight into the processes involved in remyelination.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Myelination and support of axonal integrity by glia
Nave, K.-A. (2010) · Nature · doi:10.1038/nature09614 · Cited by 1,062
de Waegh, S. M., Lee, V. M.-Y., & Brady, S. T. (1992) · Cell · doi:10.1016/0092-8674(92)90183-d · Cited by 742
Axon-Glial Signaling and the Glial Support of Axon Function
Nave, K.-A., & Trapp, B. D. (2008) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.30.051606.094309 · Cited by 658
WAXMAN, S. G., & BENNETT, M. V. L. (1972) · Nature New Biology · doi:10.1038/newbio238217a0 · Cited by 444
Grimes, M. L., Zhou, J., Beattie, E. C., Yuen, E. C., Hall, D. E., Valletta, J. S., Topp, K. S., LaVail, J. H., Bunnett, N. W., & Mobley, W. C. (1996) · The Journal of Neuroscience · doi:10.1523/jneurosci.16-24-07950.1996 · Cited by 437
NGF Controls Axonal Receptivity to Myelination by Schwann Cells or Oligodendrocytes
Chan, J. R., Watkins, T. A., Cosgaya, J. M., Zhang, C., Chen, L., Reichardt, L. F., Shooter, E. M., & Barres, B. A. (2004) · Neuron · doi:10.1016/j.neuron.2004.06.024 · Cited by 323
Self-citationRuit, K. G., Elliott, J. L., Osborne, P. A., Yan, Q., & Snider, W. D. (1992) · Neuron · doi:10.1016/0896-6273(92)90284-k · Cited by 241
Chong, S. Y. C., Rosenberg, S. S., Fancy, S. P. J., Zhao, C., Shen, Y.-A. A., Hahn, A. T., McGee, A. W., Xu, X., Zheng, B., Zhang, L. I., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1113540109 · Cited by 232
Self-citationThe geometric and spatial constraints of the microenvironment induce oligodendrocyte differentiation
Rosenberg, S. S., Kelland, E. E., Tokar, E., De La Torre, A. R., & Chan, J. R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805640105 · Cited by 226
Self-citationTarget size regulates calibre and myelination of sympathetic axons
Voyvodic, J. T. (1989) · Nature · doi:10.1038/342430a0 · Cited by 226
Cited by
The nature of feelings: evolutionary and neurobiological origins
Damasio, A., & Carvalho, G. B. (2013) · Nature Reviews Neuroscience · doi:10.1038/nrn3403 · Cited by 1,280
Myelination of the Nervous System: Mechanisms and Functions
Nave, K.-A., & Werner, H. B. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100913-013101 · Cited by 983
Oligodendrocytes in development, myelin generation and beyond
Kuhn, S., Gritti, L., Crooks, D., & Dombrowski, Y. (2019) · Cells · doi:10.3390/cells8111424 · Cited by 625
Neuronal activity biases axon selection for myelination in vivo
Hines, J. H., Ravanelli, A. M., Schwindt, R., Scott, E. K., & Appel, B. (2015) · Nature Neuroscience · doi:10.1038/nn.3992 · Cited by 454
Ehrlich, M., Mozafari, S., Glatza, M., Starost, L., Velychko, S., Hallmann, A.-L., Cui, Q.-L., Schambach, A., Kim, K.-P., Bachelin, C., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1614412114 · Cited by 257
The Axon-Myelin Unit in Development and Degenerative Disease
Stassart, R. M., Möbius, W., Nave, K.-A., & Edgar, J. M. (2018) · Frontiers in Neuroscience · doi:10.3389/fnins.2018.00467 · Cited by 246
Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination
Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M. S., Madhavan, M., Allan, K. C., Shick, H. E., Garrison, E., T. Karl, M., Factor, D. C., et al. (2018) · Nature · doi:10.1038/s41586-018-0360-3 · Cited by 226
On Myelinated Axon Plasticity and Neuronal Circuit Formation and Function
Almeida, R. G., & Lyons, D. A. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.3185-16.2017 · Cited by 221
Jha, M. K., & Morrison, B. M. (2018) · Experimental Neurology · doi:10.1016/j.expneurol.2018.07.009 · Cited by 203
The node of Ranvier in CNS pathology
Arancibia-Carcamo, I. L., & Attwell, D. (2014) · Acta Neuropathologica · doi:10.1007/s00401-014-1305-z · Cited by 177
Figlia, G., Gerber, D., & Suter, U. (2018) · Glia · doi:10.1002/glia.23273 · Cited by 165
Myelinogenic Plasticity of Oligodendrocyte Precursor Cells following Spinal Cord Contusion Injury
Assinck, P., Duncan, G. J., Plemel, J. R., Lee, M. J., Stratton, J. A., Manesh, S. B., Liu, J., Ramer, L. M., Kang, S. H., Bergles, D. E., et al. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.2409-16.2017 · Cited by 151
Regulation of myelination by microglia
Santos, E. N., & Fields, R. D. (2021) · Science Advances · doi:10.1126/sciadv.abk1131 · Cited by 137
Diversity Matters: A Revised Guide to Myelination
Tomassy, G. S., Dershowitz, L. B., & Arlotta, P. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.09.002 · Cited by 115
Baxi, E. G., DeBruin, J., Tosi, D. M., Grishkan, I. V., Smith, M. D., Kirby, L. A., Strasburger, H. J., Fairchild, A. N., Calabresi, P. A., & Gocke, A. R. (2015) · The Journal of Neuroscience · doi:10.1523/jneurosci.3817-14.2015 · Cited by 103
Call, C. L., & Bergles, D. E. (2021) · Nature Communications · doi:10.1038/s41467-021-25035-2 · Cited by 95
Specification, plasticity and evolutionary origin of peripheral glial cells
Kastriti, M. E., & Adameyko, I. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2017.11.004 · Cited by 72
Myelin replacement triggered by single-cell demyelination in mouse cortex
Snaidero, N., Schifferer, M., Mezydlo, A., Zalc, B., Kerschensteiner, M., & Misgeld, T. (2020) · Nature Communications · doi:10.1038/s41467-020-18632-0 · Cited by 63
Ephrin‐ A 1‐ EphA4 signaling negatively regulates myelination in the central nervous system
Harboe, M., Torvund‐Jensen, J., Kjaer‐Sorensen, K., & Laursen, L. S. (2018) · Glia · doi:10.1002/glia.23293 · Cited by 56
Irfan, M., Evonuk, K. S., & DeSilva, T. M. (2022) · The FEBS Journal · doi:10.1111/febs.16190 · Cited by 39
Models for Studying Myelination, Demyelination and Remyelination
Osorio-Querejeta, I., Sáenz-Cuesta, M., Muñoz-Culla, M., & Otaegui, D. (2017) · NeuroMolecular Medicine · doi:10.1007/s12017-017-8442-1 · Cited by 38
Scheinok, T. J., D’Haeseleer, M., Nagels, G., De Bundel, D., & Van Schependom, J. (2023) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2023.102459 · Cited by 16
A paradigm shift: Bioengineering meets mechanobiology towards overcoming remyelination failure
Carvalho, E. D., Morais, M. R. G., Ferreira, H. P., Silva, M. M. C., Guimarães, S. C., & Pêgo, A. P. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121427 · Cited by 16
TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration
Zhu, K., Liu, Y., Min, J.-H., Joshua, V., Lin, J., Li, Y., Kreutzmann, J. C., Guo, Y., Xia, W., Mohammadi, E., et al. (2026) · Nature Neuroscience · doi:10.1038/s41593-025-02161-4 · Cited by 11
Jagielska, A., Radzwill, K., Espinosa-Hoyos, D., Yang, M., Kowsari, K., Farley, J. E., Giera, S., Byrne, A., Sheng, G., Fang, N. X., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-44675-6 · Cited by 10
Zhang, Y., Cheng, Z., Huang, S., Sharma, T., Barbosa, D., Yu, G., & Sun, L. O. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.116252 · Cited by 4
Mazur, R. A., & Lampe, K. J. (2025) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c01455 · Cited by 3
Tsarouchas, T. M., Zoupi, L., Williams, A., & Gibson, E. M. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103609 · Cited by 2
Metadata
- Authors
- Lee, S., Leach, M. K., Redmond, S. A., Chong, S. Y. C., Mellon, S. H., Tuck, S. J., Feng, Z.-Q., Corey, J. M., & Chan, J. R.
- Venue
- Nature Methods
- Published
- 2012-07-15
- Volume
- 9
- Issue
- 9
- Pages
- 917-922
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1975719696
- Cited by
- 434
- References
- 27
- FWCI
- 6.56
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
