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Massively multiplex single-cell Hi-C
Open accessAbstract
We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizable strategy for single-cell genomics.
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Metadata
- Authors
- Ramani, V., Deng, X., Qiu, R., Gunderson, K. L., Steemers, F. J., Disteche, C. M., Noble, W. S., Duan, Z., & Shendure, J.
- Venue
- Nature Methods
- Published
- 2017-01-30
- Volume
- 14
- Issue
- 3
- Pages
- 263-266
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2582946425
- Cited by
- 546
- References
- 13
- FWCI
- 22.71
- Type
- journal article
- Language
- en
- Open access
- green
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