Cited by 382Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsHypothalamic control of reproductive hormones

A genomic atlas of mouse hypothalamic development

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Shimogori, Tomomi, Lee, Daniel A, Miranda-Angulo, Ana, Yang, Yanqin, Wang, Hong, Jiang, Lizhi, Yoshida, Aya C, Kataoka, Ayane +5 more·2010·Nature Neuroscience

Abstract

The hypothalamus is a central regulator of many behaviors that are essential for survival, such as temperature regulation, food intake and circadian rhythms. However, the molecular pathways that mediate hypothalamic development are largely unknown. To identify genes expressed in developing mouse hypothalamus, we performed microarray analysis at 12 different developmental time points. We then conducted developmental in situ hybridization for 1,045 genes that were dynamically expressed over the course of hypothalamic neurogenesis. We identified markers that stably labeled each major hypothalamic nucleus over the entire course of neurogenesis and constructed a detailed molecular atlas of the developing hypothalamus. As a proof of concept of the utility of these data, we used these markers to analyze the phenotype of mice in which Sonic Hedgehog (Shh) was selectively deleted from hypothalamic neuroepithelium and found that Shh is essential for anterior hypothalamic patterning. Our results serve as a resource for functional investigations of hypothalamic development, connectivity, physiology and dysfunction.

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Crossref · 10.1038/nn.2545

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  • Both RT and ZI neurons may have originated from the prethalamus or the zona limitans intrathalamica.

    “Both RT and ZI neurons may have originated from the prethalamus or the zona limitans intrathalamica (ZLI)34–38.”

    From A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain (2023) · cites this paper as [38]

    The Results section on the anterior diencephalon describes markers of prethalamic neuroepithelium, and the section on basal hypothalamic Shh discusses the ZLI. Neither passage addresses whether RT or ZI neurons originate from either region. Searches for reticular nucleus and zona incerta did not locate a passage establishing that origin. (LLM, conf=0.72)

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Authors
Shimogori, T., Lee, D. A., Miranda-Angulo, A., Yang, Y., Wang, H., Jiang, L., Yoshida, A. C., Kataoka, A., Mashiko, H., Avetisyan, M., Qi, L., Qian, J., & Blackshaw, S.
Affiliations
  • RIKEN (JP)
  • Johns Hopkins University (US)
Corresponding author
  • Shimogori, Tomomi
  • Blackshaw, Seth
Venue
Nature Neuroscience
Published
2010-05-02
Volume
13
Issue
6
Pages
767-775
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2092223956
Cited by
382
References
50
Citations by year
2026: 12 · 2025: 15 · 2024: 12 · 2023: 20 · 2022: 24 · 2021: 29 · 2020: 40 · 2019: 22 · 2018: 24 · 2017: 18 · 2016: 26 · 2015: 41 · 2014: 17 · 2013: 36 · 2012: 22
FWCI
10.69
Type
journal article
Language
en
Open access
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repository University of Antioquia (University of Antioquia) (publishedVersion, cc-by)
  • PubMed Central (acceptedVersion)
Funders
  • Johns Hopkins University
  • RIKEN
  • National Institute of Neurological Disorders and Stroke (R21NS067393)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords
hypothalamic development, hypothalamic nuclei, neurogenesis, gene expression atlas, Sonic hedgehog, tuberal hypothalamus, basal plate, developmental markers, mouse embryo
MeSH terms
Animals, Atlases as Topic, Diencephalon, Female, Hypothalamus, Male, Mice, Inbred C57BL, Mice, Transgenic, Phenotype, Sex Characteristics, Species Specificity, Telencephalon, Reproducibility of Results, Genome, In Situ Hybridization, Gene Expression Regulation, Developmental, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Neuroepithelial Cells, Mice, Hedgehog Proteins, Neurogenesis

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