High-throughput functional genomics using CRISPR-Cas9

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Items 301–400 of 1,000

1. Systems immunology: Integrating multi-omics data to infer regulatory networks and hidden drivers of immunity
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2. Discovering and validating cancer genetic dependencies: approaches and pitfalls
   Ann Lin, Jason M. Sheltzer · 2020 · Nature Reviews Genetics
3. A potential paradigm in CRISPR/Cas systems delivery: at the crossroad of microalgal gene editing and algal-mediated nanoparticles
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4. Global insights into acetic acid resistance mechanisms and genetic stability of Acetobacter pasteurianus strains by comparative genomics
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5. gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection
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6. Challenges and Strategies in Ascribing Functions to Long Noncoding RNAs
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7. Exploring liver cancer biology through functional genetic screens
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8. Computer vision for high content screening
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9. Site-Specific and Enzymatic Cross-Linking of sgRNA Enables Wavelength-Selectable Photoactivated Control of CRISPR Gene Editing
   Dongyang Zhang, Luping Liu, Shuaijiang Jin, Ember M. Tota, Zijie Li, Xijun Piao, Xuan Zhang, Xiang‐Dong Fu, Neal K. Devaraj · 2022 · Journal of the American Chemical Society
10. Loss of KMT5C Promotes EGFR Inhibitor Resistance in NSCLC via LINC01510-Mediated Upregulation of MET
    Arpita S. Pal, Alejandra Agredo, Nadia A. Lanman, Jihye Son, Ikjot Singh Sohal, Manvir Bains, Chennan Li, Jenna Clingerman, Kayla Gates, Andrea L. Kasinski · 2022 · Cancer Research
11. Optimizing multiplex CRISPR/Cas9-based genome editing for wheat
    Wei Wang, Alina R. Akhunova, Shiaoman Chao, Eduard Akhunov · 2016 · bioRxiv (Cold Spring Harbor Laboratory)
12. Cholesterol-rich lipid-mediated nanoparticles boost of transfection efficiency, utilized for gene editing by CRISPR-Cas9
    Elaheh Sadat Hosseini, Maryam Nikkhah, Saman Hosseinkhani · 2019 · International Journal of Nanomedicine
13. Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer
    Macus Hao‐Ran Bao, Chunxue Yang, Aki Pui‐Wah Tse, Lai Wei, Derek Lee, Misty Shuo Zhang, Chi Ching Goh, David Kung‐Chun Chiu, Vincent Wai‐Hin Yuen, Cheuk‐Ting Law et al. · 2021 · Cell Reports
14. Multiplexed promoter and gene editing in wheat using a virus‐based guide RNA delivery system
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15. rVarBase: an updated database for regulatory features of human variants
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16. Towards a compendium of essential genes – From model organisms to synthetic lethality in cancer cells
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17. CRISPR/Cas9: From Genome Engineering to Cancer Drug Discovery
    Ji Luo · 2016 · Trends in cancer
18. PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing
    Abba Malina, Christopher J. F. Cameron, Francis Robert, Mathieu Blanchette, Josée Dostie, Jerry Pelletier · 2015 · Nature Communications
19. Systems biology of lens development: A paradigm for disease gene discovery in the eye
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20. Integrated genetic and pharmacologic interrogation of rare cancers
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21. Dual direction CRISPR transcriptional regulation screening uncovers gene networks driving drug resistance
    Carlos le Sage, Steffen Lawo, Prince Panicker, Tim M. E. Scales, Syed Asad Rahman, Annette S. Little, Nicola McCarthy, Jonathan D. Moore, Benedict C. S. Cross · 2017 · Scientific Reports
22. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
    Jiazhang Lian, Mohammad HamediRad, Huimin Zhao · 2018 · Biotechnology Journal
23. Functional Toxicogenomic Assessment of Triclosan in Human HepG2 Cells Using Genome-Wide CRISPR-Cas9 Screening
    Pu Xia, Xiaowei Zhang, Yuwei Xie, Miao Guan, Daniel L. Villeneuve, Hongxia Yu · 2016 · Environmental Science & Technology
24. Towards quantitative and multiplexed in vivo functional cancer genomics
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25. Aptamer Chimeras for Therapeutic Delivery: The Challenging Perspectives
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26. Moving from in vitro to in vivo CRISPR screens
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27. Single-Cell Mechanics: Structural Determinants and Functional Relevance
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28. The Emerging Roles of Forkhead Box (FOX) Proteins in Osteosarcoma
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29. Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance
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30. DNA methylation in epigenetic inheritance of metabolic diseases through the male germ line
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31. Modulating intracellular pathways to improve non-viral delivery of RNA therapeutics
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32. H3K9 methylation drives resistance to androgen receptor–antagonist therapy in prostate cancer
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33. High-resolution genome-wide mapping of chromosome-arm-scale truncations induced by CRISPR–Cas9 editing
    Nathan H. Lazar, Safiye Çelik, Lu Chen, Marta M. Fay, Jonathan C. Irish, James Jensen, Conor A. Tillinghast, John Urbanik, William P. Bone, Genevieve H. L. Roberts et al. · 2024 · Nature Genetics
34. Gene therapy for diabetic retinopathy: Are we ready to make the leap from bench to bedside?
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35. TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network
    Seruggia, Davide, Oti, Martin, Tripathi, Pratibha, Canver, Matthew C., LeBlanc, Lucy, Di Giammartino, Dafne C., Bullen, Michael J., Nefzger, Christian M., Sun, Yu Bo Yang, Farouni, Rick et al. · 2019 · Molecular Cell
36. The Functional Association of ACQOS/VICTR with Salt Stress Resistance in Arabidopsis thaliana Was Confirmed by CRISPR-Mediated Mutagenesis
    Sang‐Tae Kim, Min‐Kyeung Choi, Su‐Ji Bae, Jin‐Soo Kim · 2021 · International Journal of Molecular Sciences
37. CRISPR/Cas9 screen uncovers functional translation of cryptic lncRNA-encoded open reading frames in human cancer
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38. Progress and Perspective of CRISPR‐Cas9 Technology in Translational Medicine
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39. CRISPR innovations in tissue engineering and gene editing
    Zahra-Sadat Razavi, Madjid Soltani, Mohammad Souri, André J. van Wijnen · 2024 · Life Sciences
40. Drugging Chromatin in Cancer: Recent Advances and Novel Approaches
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41. CRISPR: a versatile tool for both forward and reverse genetics research
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42. Network Approaches for Dissecting the Immune System
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43. Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing
    Dongyoon Kim, Quoc‐Viet Le, Yina Wu, Jin-Won Park, Yu‐Kyoung Oh · 2020 · Pharmaceutics
44. Theragnostic application of nanoparticle and CRISPR against food-borne multi-drug resistant pathogens
    Rahul Bhattacharjee, Aditya Nandi, Priya Mitra, Koustav Saha, Paritosh Patel, Ealisha Jha, Pritam Kumar Panda, Sushil Kumar Singh, Ateet Dutt, Yogendra Kumar Mishra et al. · 2022 · Materials Today Bio
45. Entry receptors — the gateway to alphavirus infection
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46. CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis
    Vivien A. C. Schoonenberg, Mitchel A. Cole, Qiuming Yao, Claudio Macias-Treviño, Falak Sher, Patrick G. Schupp, Matthew C. Canver, Takahiro Maeda, Luca Pinello, Daniel E. Bauer · 2018 · Genome biology
47. Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans
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48. Insights into maize genome editing via CRISPR/Cas9
    Astha Agarwal, Pranjal Yadava, Krishan Kumar, Ishwar Singh, Tanushri Kaul, A. Pattanayak, Pawan Kumar Agrawal · 2018 · Physiology and Molecular Biology of Plants
49. CRISPR screen identifies genes that sensitize AML cells to double-negative T-cell therapy
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50. The economics and policy of genome editing in crop improvement
    Nicholas Kalaitzandonakes, Christopher J. Willig, K. Zahringer · 2022 · The Plant Genome
51. The design and engineering of synthetic genomes
    Joshua S. James, Junbiao Dai, Wei Leong Chew, Yizhi Cai · 2024 · Nature Reviews Genetics
52. Genomic targeting of epigenetic probes using a chemically tailored Cas9 system
    Glen Liszczak, Zachary Z. Brown, Samuel H. Kim, Rob Oslund, Yael David, Tom W. Muir · 2017 · Proceedings of the National Academy of Sciences
53. A Perspective on Implementing a Quantitative Systems Pharmacology Platform for Drug Discovery and the Advancement of Personalized Medicine
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54. Large-scale genome editing in plants: approaches, applications, and future perspectives
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55. Genome-Wide Interrogation of Human Cancers Identifies EGLN1 Dependency in Clear Cell Ovarian Cancers
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56. High-resolution characterization of gene function using single-cell CRISPR tiling screen
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57. Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
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58. Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries
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59. Principles and methods of in-silico prioritization of non-coding regulatory variants
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60. The APOBEC3 genes and their role in cancer: insights from human papillomavirus
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61. Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens
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62. Programming Morphogenesis through Systems and Synthetic Biology
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63. An automated microfluidic gene-editing platform for deciphering cancer genes
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64. Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease
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65. The African Turquoise Killifish: A Model for Exploring Vertebrate Aging and Diseases in the Fast Lane
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66. ZNF207-driven PRDX1 lactylation and NRF2 activation in regorafenib resistance and ferroptosis evasion
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67. Long non-coding RNA Lucat1 is a poor prognostic factor and demonstrates malignant biological behavior in clear cell renal cell carcinoma
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68. New tools for old drugs: Functional genetic screens to optimize current chemotherapy
    Nora M. Gerhards, Sven Rottenberg · 2018 · Drug Resistance Updates
69. Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens
    Jean‐Philippe Fortin, Jenille Tan, Karen E. Gascoigne, Peter M. Haverty, William F. Forrest, Michael Rodrigues Costa, Scott E. Martin · 2019 · Genome biology
70. Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice
    Ying Chieh Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang · 2019 · Frontiers in Genetics
71. VNN disease and status of breeding for resistance to NNV in aquaculture
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72. nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation
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73. The Most Developmentally Truncated Fishes Show Extensive Hox Gene Loss and Miniaturized Genomes
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74. CRISPR/Cas9 as a therapeutic tool for triple negative breast cancer: from bench to clinics
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75. Megabase-scale human genome rearrangement with programmable bridge recombinases
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76. Host-targeting agents for treatment of hepatitis B virus infection
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77. CRISPR Screens to Discover Functional Noncoding Elements
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78. A Comparison of Techniques to Evaluate the Effectiveness of Genome Editing
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79. lncRedibly versatile: biochemical and biological functions of long noncoding RNAs
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80. CRISPR knockout screen implicates three genes in lysosome function
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81. Therapeutic genome editing in cardiovascular diseases
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82. Massively Parallel CRISPR‐Based Genetic Perturbation Screening at Single‐Cell Resolution
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83. CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
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84. CRISPR-Mediated Epigenome Editing.
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85. Generation of an immortalized erythroid progenitor cell line from peripheral blood: A model system for the functional analysis of Plasmodium spp. invasion
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86. Using phenotypic plasticity to understand the structure and evolution of the genotype–phenotype map
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87. CRISPR/Cas9 editing reveals novel mechanisms of clustered microRNA regulation and function
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88. Improvement of glucosinolates by metabolic engineering in Brassica crops
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89. Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy
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90. CRISPR–Cas9 gene editing induced complex on-target outcomes in human cells
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91. Targeting long non-coding RNAs in cancer therapy using CRISPR-Cas9 technology: A novel paradigm for precision oncology
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92. CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line.
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93. Finding functional microproteins
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94. Increasing the performance of pooled CRISPR–Cas9 drop-out screening
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95. TAZ induces lung cancer stem cell properties and tumorigenesis by up-regulating ALDH1A1
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96. CRISPR/Cas9 based genome editing for targeted transcriptional control in triple-negative breast cancer
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97. Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges
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99. Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic Fungus Myceliophthora thermophila
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100. No country for old methods: New tools for studying microproteins
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