High-throughput functional genomics using CRISPR-Cas9

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Bartel (2004) · doi:10.1016/s0092-8674(04)00045-5 · Cited by 34,714

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Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

MicroRNAs: small RNAs with a big role in gene regulation

He, L., & Hannon, G. J. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1379 · Cited by 7,118

Development and Applications of CRISPR-Cas9 for Genome Engineering

Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762

Improved vectors and genome-wide libraries for CRISPR screening

Sanjana, N. E., Shalem, O., & Zhang, F. (2014) · Nature Methods · doi:10.1038/nmeth.3047 · Cited by 5,239

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Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234

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Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016

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Sagi, I., Egli, D. M., & Benvenisty, N. (2016) · Nature Protocols · doi:10.1038/nprot.2016.145 · Cited by 13

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Wu, Q., Ferry, Q. R. V., Baeumler, T. A., Michaels, Y. S., Vitsios, D., Habib, O., Arnold, R. R., Jiang, X., Maio, S., Steinkraus, B. R., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00686-2 · Cited by 13

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Cachera, P., Olsson, H., Coumou, H. C., Jensen, M. L., Sánchez, B. J., Strucko, T., van den Broek, M. A., Daran, J.-M. G., Jensen, M. K., Sonnenschein, N., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad656 · Cited by 8

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Mondal, P., Alyateem, G., Mitchell, A. V., & Gottesman, M. M. (2024) · Scientific Reports · doi:10.1038/s41598-024-66244-1 · Cited by 8

CRISPR-based genetic screens advance cancer immunology

Cao, Y., Li, X., Pan, Y., Wang, H., Yang, S., Hong, L., & Ye, L. (2024) · Science China Life Sciences · doi:10.1007/s11427-023-2571-0 · Cited by 8

Recent advances in CRISPR-Cas systems for colorectal cancer research and therapeutics

Sokhangouy, S. K., Alizadeh, F. L., Lotfi, M., Sharif, S., Ashouri, A., Yoosefi, Y., Qomi, S. B., & Abbaszadegan, M. R. (2024) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2024.2388777 · Cited by 8

Exploring the role of FAT genes in Solanaceae species through genome‐wide analysis and genome editing

Bahadır, S., Abdulla, M. F., Mostafa, K., Kavas, M., Hacıkamiloğlu, M. S., Kurt, O., & Yıldırım, K. (2024) · The Plant Genome · doi:10.1002/tpg2.20506 · Cited by 8

CRISPR/Cas9, a universal tool for genomic engineering

Smirnov, A. V., Yunusova, A., Lukyanchikova, V., & Баттулин, Н. Р. (2017) · Russian Journal of Genetics Applied Research · doi:10.1134/s2079059717040116 · Cited by 9

Next-generation omics technologies to explore microbial diversity

Sharma, A., Vashistt, J., & Shrivastava, R. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-824448-7.00026-7 · Cited by 8

Ribosomal stress induces 2-cell embryo-like state transition of the mouse ESCs through p53 activation

Maeda, T., Kimura, Y., Nakano, T., & Yamaguchi, S. (2021) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2021.09.068 · Cited by 8

Rare Does Not Mean Worthless: How Rare Diseases Have Shaped Neurodevelopment Research in the NGS Era

Zaghi, M., Banfi, F., Bellini, E. N., & Sessa, A. (2021) · Biomolecules · doi:10.3390/biom11111713 · Cited by 8

Integrated genome and tissue engineering enables screening of cancer vulnerabilities in physiologically relevant perfusable ex vivo cultures

Hu, M. Y., Lei, X. Y., Larson, J. D., McAlonis‐Downes, M., Ford, K. M., McDonald, D., Mach, K., Rusert, J. M., Wechsler‐Reya, R. J., & Mali, P. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121276 · Cited by 8

Generation of a recessive dystrophic epidermolysis bullosa mouse model with patient-derived compound heterozygous mutations

Takaki, S., Shimbo, T., Ikegami, K., Kitayama, T., Yamamoto, Y., Yamazaki, S., Mori, S., & Tamai, K. (2022) · Laboratory Investigation · doi:10.1038/s41374-022-00735-5 · Cited by 8

Identifying Lethal Dependencies with HUGE Predictive Power

Gimeno, M., José‐Eneriz, E. S., Rubio, A., Gárate, L., Miranda, E., Castilla, C., Agirre, X., Prósper, F., & Carazo, F. (2022) · Cancers · doi:10.3390/cancers14133251 · Cited by 8

Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention

Scarborough, A. M., Govindan, A., & Conrad, N. K. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2521-7_16 · Cited by 8

CRISPR-Cas9 mediated genome editing of Huntington’s disease neurospheres

Han, J. Y., Seo, J., Choi, Y., Im, W., Ban, J., & Sung, J. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-08175-6 · Cited by 8

The N-peptide–binding mode is critical to Munc18-1 function in synaptic exocytosis

Shen, C., Liu, Y., Yu, H., Gulbranson, D. R., Kogut, I., Bilousova, G., Zhang, C., Stowell, M. H. B., & Shen, J. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.005254 · Cited by 9

Genetic screens and functional genomics using CRISPR /Cas9 technology

Hartenian, E., & Doench, J. G. (2015) · FEBS Journal · doi:10.1111/febs.13248 · Cited by 100

High‐throughput screens in mammalian cells using the CRISPR‐Cas9 system

Peng, J., Zhou, Y., Zhu, S., & Wei, W. (2015) · FEBS Journal · doi:10.1111/febs.13251 · Cited by 60

Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

Joung, J., Konermann, S., Gootenberg, J. S., Abudayyeh, O. O., Platt, R. J., Brigham, M. D., Sanjana, N. E., & Zhang, F. (2017) · Nature Protocols · doi:10.1038/nprot.2017.016 · Cited by 1,243

The Current Situation and Development Prospect of Whole-Genome Screening

Yang, C., Lei, Y., Ren, T., & Yao, M. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25010658 · Cited by 12

Prion Diseases

Pagel, M. D. (1996) · Trends in Neurosciences · doi:10.1016/s0166-2236(96)60033-2