Cited by 7193D Printing in Biomedical ResearchCellular Mechanics and InteractionsCancer Cells and Metastasis

The DOI matches its Crossref record; 199 of the 199 references it cites were found in a registry or an index.

Three-dimensional organotypic culture: experimental models of mammalian biology and disease

Open access

Shamir, Eliah R., Ewald, Andrew J.·2014·Nature Reviews Molecular Cell Biology

Open this paper in CoChat — free account

Abstract

Mammalian organs are challenging to study as they are fairly inaccessible to experimental manipulation and optical observation. Recent advances in three-dimensional (3D) culture techniques, coupled with the ability to independently manipulate genetic and microenvironmental factors, have enabled the real-time study of mammalian tissues. These systems have been used to visualize the cellular basis of epithelial morphogenesis, to test the roles of specific genes in regulating cell behaviours within epithelial tissues and to elucidate the contribution of microenvironmental factors to normal and disease processes. Collectively, these novel models can be used to answer fundamental biological questions and generate replacement human tissues, and they enable testing of novel therapeutic approaches, often using patient-derived cells.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nrm3873

Cites

199 of 199 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Molecular Mechanisms of Stress-Responsive Changes in Collagen and Elastin Networks in Skin
Aziz, J., Shezali, H., Radzi, Z., Yahya, N. A., Abu Kassim, N. H., Czernuszka, J., & Rahman, M. T. (2016) · Skin Pharmacology and Physiology · doi:10.1159/000447017 · Cited by 7,323
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Molecular Biology of the Cell
Alberts, B., Johnson, A. D., Lewis, J. H., RAFF, M. C., Roberts, K. J., & Walter, P. (2007) · W.W. Norton eBooks · doi:10.1201/9780203833445 · Cited by 4,591
Reconstituting organ-level lung functions on a chip
Huh, D., Matthews, B. D., Mammoto, A., Montoya-Zavala, M., Hsin, H. Y., & Ingber, D. E. (2010) · Science · doi:10.1126/science.1188302 · Cited by 4,250
Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling
Levental, K. R., Yu, H., Kass, L., Lakins, J. N., Egeblad, M., Erler, J. T., Fong, S. F. T., Csiszar, K., Giaccia, A., Weninger, W., et al. (2009) · Cell · doi:10.1016/j.cell.2009.10.027 · Cited by 4,101
Tensional homeostasis and the malignant phenotype
Paszek, M. J., Zahir, N., Johnson, K. R., Lakins, J. N., Rozenberg, G. I., Gefen, A., Reinhart-King, C. A., Margulies, S. S., Dembo, M., Boettiger, D., et al. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.08.010 · Cited by 3,941
Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium
Sato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753

Show all 199 references

Research works printed but not linked: 2

Bichat, X. General Anatomy, Applied to Physiology and Medicine. (Richardson and Lord, 1822).
Nguyen-Ngoc, K. V. et al. in Tissue Morphogenesis: Methods and Protocols Vol. 1189 Methods in Molecular Biology (ed. Nelson, C. M.) (Springer Science and Business Media, 2014).

Sorted by citations

Three-dimensional in vitro cell culture models in drug discovery and drug repositioning
Langhans, S. A. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00006 · Cited by 1,522
Organoids as an in vitro model of human development and disease
Fatehullah, A., Tan, S. H., & Barker, N. (2016) · Nature Cell Biology · doi:10.1038/ncb3312 · Cited by 1,508
Human primary liver cancer–derived organoid cultures for disease modeling and drug screening
Broutier, L., Mastrogiovanni, G., Verstegen, M. M., Francies, H. E., Gavarró, L. M., Bradshaw, C. R., Allen, G. E., Arnes-Benito, R., Sidorova, O., Gaspersz, M. P., et al. (2017) · Nature Medicine · doi:10.1038/nm.4438 · Cited by 1,471
Organs-on-chips at the frontiers of drug discovery
Esch, E. W., Bahinski, A., & Huh, D. (2015) · Nature Reviews Drug Discovery · doi:10.1038/nrd4539 · Cited by 1,185
Three-Dimensional Cell Cultures in Drug Discovery and Development
Fang, Y., & Eglen, R. M. (2017) · SLAS Discovery · doi:10.1177/1087057117696795 · Cited by 849
Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening
Kim, M., Mun, H., Sung, C. O., Cho, E. J., Jeon, H.-J., Chun, S.-M., Jung, D. J., Shin, T. H., Jeong, G. S., Kim, D. K., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11867-6 · Cited by 812
Modes of cancer cell invasion and the role of the microenvironment
Clark, A. G., & Vignjevic, D. M. (2015) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2015.06.004 · Cited by 788
A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo
Hubert, C. G., Rivera, M., Spangler, L. C., Wu, Q., Mack, S. C., Prager, B. C., Couce, M., McLendon, R. E., Sloan, A. E., & Rich, J. N. (2016) · Cancer Research · doi:10.1158/0008-5472.can-15-2402 · Cited by 673
Organoids: A historical perspective of thinking in three dimensions
Simian, M., & Bissell, M. J. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201610056 · Cited by 620
Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate Alzheimer’s Disease Phenotypes
Raja, W. K., Mungenast, A. E., Lin, Y.-T., Ko, T., Abdurrob, F., Seo, J., & Tsai, L.-H. (2016) · PLOS ONE · doi:10.1371/journal.pone.0161969 · Cited by 569
Screening out irrelevant cell-based models of disease
Horvath, P., Aulner, N., Bickle, M., Davies, A. M., Nery, E. D., Ebner, D., Montoya, M. C., Östling, P., Pietiäinen, V., Price, L. S., et al. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.175 · Cited by 525
Organotypic brain slice cultures: A review
Humpel, C. (2015) · Neuroscience · doi:10.1016/j.neuroscience.2015.07.086 · Cited by 480
Organoids by design
Takebe, T., & Wells, J. M. (2019) · Science · doi:10.1126/science.aaw7567 · Cited by 417
Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models
Tsai, S., McOlash, L., Palen, K., Johnson, B., Duris, C., Yang, Q., Dwinell, M. B., Hunt, B., Evans, D. B., Gershan, J., et al. (2018) · BMC Cancer · doi:10.1186/s12885-018-4238-4 · Cited by 400
Vascularized cancer on a chip: the effect of perfusion on growth and drug delivery of tumor spheroid
Nashimoto, Y., Okada, R., Hanada, S., Arima, Y., Nishiyama, K., Miura, T., & Yokokawa, R. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119547 · Cited by 332
Microenvironmental Determinants of Pancreatic Cancer
Heßmann, E., Buchholz, S., Demir, İ. E., Singh, S. K., Gress, T. M., Ellenrieder, V., & Neeße, A. (2020) · Physiological Reviews · doi:10.1152/physrev.00042.2019 · Cited by 336
Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids
Marsee, A., Roos, F. J. M., Verstegen, M. M. A., Marsee, A., Roos, F., Verstegen, M., Clevers, H., Vallier, L., Takebe, T., Huch, M., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.04.005 · Cited by 310
Tumor organoids: applications in cancer modeling and potentials in precision medicine
Xu, H., Jiao, D., Liu, A., & Wu, K. (2022) · Journal of Hematology & Oncology · doi:10.1186/s13045-022-01278-4 · Cited by 286
3D neural tissue models: From spheroids to bioprinting
Zhuang, P., Sun, A. X., An, J., Chua, C. K., & Chew, S. Y. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.002 · Cited by 269
Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device
Nashimoto, Y., Hayashi, T., Kunita, I., Nakamasu, A., Torisawa, Y.-S., Nakayama, M., Takigawa-Imamura, H., Kotera, H., Nishiyama, K., Miura, T., et al. (2017) · Integrative Biology · doi:10.1039/c7ib00024c · Cited by 266
Microbiome in Colorectal Cancer: How to Get from Meta-omics to Mechanism?
Ternes, D., Karta, J., Tsenkova, M., Wilmes, P., Haan, S., & Letellier, E. (2020) · Trends in Microbiology · doi:10.1016/j.tim.2020.01.001 · Cited by 267
Organoid Models of Human Gastrointestinal Development and Disease
Dedhia, P. H., Bertaux‐Skeirik, N., Zavros, Y. L., & Spence, J. R. (2016) · Gastroenterology · doi:10.1053/j.gastro.2015.12.042 · Cited by 252
Programmed synthesis of three-dimensional tissues
Todhunter, M. E., Jee, N. Y., Hughes, A. J., Coyle, M. C., Cerchiari, A. E., Farlow, J., Garbe, J. C., LaBarge, M. A., Desai, T. A., & Gartner, Z. J. (2015) · Nature Methods · doi:10.1038/nmeth.3553 · Cited by 246
Organ‐on‐chip models: Implications in drug discovery and clinical applications
Mittal, R., Woo, F. W., Castro, C. S., Cohen, M. A., Karanxha, J., Mittal, J., Chhibber, T., & Jhaveri, V. M. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.27729 · Cited by 244
Multiplexed Epitope-Based Tissue Imaging for Discovery and Healthcare Applications
Bodenmiller, B. (2016) · Cell Systems · doi:10.1016/j.cels.2016.03.008 · Cited by 235
Stem cell-derived organoids and their application for medical research and patient treatment
Bartfeld, S., & Clevers, H. C. (2017) · Journal of Molecular Medicine · doi:10.1007/s00109-017-1531-7 · Cited by 226
Organoids for Drug Discovery and Personalized Medicine
Takahashi, T. (2018) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-010818-021108 · Cited by 210
Chemoresistance of Cancer Cells: Requirements of Tumor Microenvironment-mimicking In Vitro Models in Anti-Cancer Drug Development
Jo, Y., Choi, N., Kim, K., Koo, H., Choi, J. H., & Kim, H. N. (2018) · Theranostics · doi:10.7150/thno.29098 · Cited by 188
In Vitro three-dimensional (3D) cell culture tools for spheroid and organoid models
Lee, S., Koo, I.-S., Hwang, H. J., & Lee, D. W. (2023) · SLAS DISCOVERY · doi:10.1016/j.slasd.2023.03.006 · Cited by 193
Advances in Engineering Human Tissue Models
Moysidou, C., Barberio, C., & Owens, R. M. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.620962 · Cited by 193
Cerebral organoids reveal early cortical maldevelopment in schizophrenia-computational anatomy and genomics, role of FGFR1
Stachowiak, E. K., Benson, C. A., Narla, S. T., Dimitri, A., Chuye, L. E. B., Dhiman, S., Harikrishnan, K., Elahi, S., Freedman, D., Brennand, K. J., et al. (2017) · Translational Psychiatry · doi:10.1038/s41398-017-0054-x · Cited by 179
Physiologically based pharmacokinetic and pharmacodynamic analysis enabled by microfluidically linked organs-on-chips
Prantil-Baun, R., Novak, R., Das, D., Somayaji, M. R., Przekwas, A., & Ingber, D. E. (2018) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-010716-104748 · Cited by 176
Testicular organoid generation by a novel in vitro three-layer gradient system
Alves-Lopes, J. P., Söder, O., & Stukenborg, J. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.03.025 · Cited by 176
Somatic cell-derived organoids as prototypes of human epithelial tissues and diseases
Fujii, M., & Sato, T. (2020) · Nature Materials · doi:10.1038/s41563-020-0754-0 · Cited by 176
Rapid 3D Extrusion of Synthetic Tumor Microenvironments
Grolman, J. M., Zhang, D., Smith, A. M., Moore, J. S., & Kilian, K. A. (2015) · Advanced Materials · doi:10.1002/adma.201501729 · Cited by 167
Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing
Bédard, P., Gauvin, S., Ferland, K., Caneparo, C., Pellerin, È., Chabaud, S., & Bolduc, S. (2020) · Bioengineering · doi:10.3390/bioengineering7030115 · Cited by 167
Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age
Barrila, J., Crabbé, A., Yang, J., Franco, K., Nydam, S. D., Forsyth, R. J., Davis, R. R., Gangaraju, S., Ott, C. M., Coyne, C. B., et al. (2018) · Infection and Immunity · doi:10.1128/iai.00282-18 · Cited by 163
The biology of beta human papillomaviruses
Tommasino, M. (2016) · Virus Research · doi:10.1016/j.virusres.2016.11.013 · Cited by 166
Engineered cell and tissue models of pulmonary fibrosis
Sundarakrishnan, A., Chen, Y. G., Black, L. D., Aldridge, B. B., & Kaplan, D. L. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.12.013 · Cited by 156
Automated brightfield morphometry of 3D organoid populations by OrganoSeg
Borten, M. A., Bajikar, S. S., Sasaki, N., Clevers, H. C., & Janes, K. A. (2018) · Scientific Reports · doi:10.1038/s41598-017-18815-8 · Cited by 150
Understanding cell‐cell communication and signaling in the colorectal cancer microenvironment
AlMusawi, S., Ahmed, M., & Nateri, A. S. (2021) · Clinical and Translational Medicine · doi:10.1002/ctm2.308 · Cited by 153
Cell polarity signaling in the plasticity of cancer cell invasiveness
Gandalovičová, A., Vomastek, T., Rösel, D., & Brábek, J. (2016) · Oncotarget · doi:10.18632/oncotarget.7214 · Cited by 156
Organ-On-A-Chip in vitro Models of the Brain and the Blood-Brain Barrier and Their Value to Study the Microbiota-Gut-Brain Axis in Neurodegeneration
Raimondi, I., Izzo, L., Tunesi, M., Comar, M., Albani, D., & Giordano, C. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00435 · Cited by 150
Emerging Biotechnology Applications of Aqueous Two‐Phase Systems
Teixeira, A. G., Agarwal, R., Ko, K. R., Grant‐Burt, J., Leung, B. M., & Frampton, J. P. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201701036 · Cited by 153
OrgaQuant: Human Intestinal Organoid Localization and Quantification Using Deep Convolutional Neural Networks
Kassis, T., Hernandez‐Gordillo, V., Langer, R., & Griffith, L. G. (2019) · Scientific Reports · doi:10.1038/s41598-019-48874-y · Cited by 141
Near‐Infrared Light‐Sensitive Polyvinyl Alcohol Hydrogel Photoresist for Spatiotemporal Control of Cell‐Instructive 3D Microenvironments
Qin, X., Wang, X., Rottmar, M., Nelson, B. J., & Maniura‐Weber, K. (2018) · Advanced Materials · doi:10.1002/adma.201705564 · Cited by 140
Organic Bioelectronics for In Vitro Systems
Pitsalidis, C., Pappa, A., Boys, A. J., Fu, Y., Moysidou, C., van Niekerk, D. C., Sáez, J., Savva, A., Iandolo, D., & Owens, R. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00539 · Cited by 141
Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes
Harms, M., Li, Q., Lee, S., Zhang, C., Kull, B., Hallén, S., Thorell, A., Alexandersson, I., Hagberg, C. E., Peng, X., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.03.026 · Cited by 137
Human airway organoids and microplastic fibers: A new exposure model for emerging contaminants
Winkler, A., Cherubini, A., Rusconi, F., Santo, N., Madaschi, L., Pistoni, C., Moschetti, G., Sarnicola, M. L., Crosti, M., Rosso, L., et al. (2022) · Environment International · doi:10.1016/j.envint.2022.107200 · Cited by 133
Synthetic hydrogels mimicking basement membrane matrices to promote cell-matrix interactions
Cruz‐Acuña, R., & Garcı́a, A. J. (2016) · Matrix Biology · doi:10.1016/j.matbio.2016.06.002 · Cited by 133
Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture
Inal, S., Hama, A., Ferro, M. P., Pitsalidis, C., Oziat, J., Iandolo, D., Pappa, A., Hadida, M., Huerta, M., Marchat, D., et al. (2017) · Advanced Biosystems · doi:10.1002/adbi.201700052 · Cited by 123
Capturing complex tumour biology in vitro: histological and molecular characterisation of precision cut slices
Davies, E. J., Dong, M., Gutekunst, M., Närhi, K. M., Zoggel, H. J. A. A. V., Blom, S., Nagaraj, A. S., Metsalu, T., Oswald, E., Erkens‐Schulze, S., et al. (2015) · Scientific Reports · doi:10.1038/srep17187 · Cited by 131
Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures
Urciuolo, A., Giobbe, G. G., Dong, Y., Michielin, F., Brandolino, L., Magnussen, M., Gagliano, O., Selmin, G., Scattolini, V., Raffa, P., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-37953-4 · Cited by 121
Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture
Ryan, S.-L., Baird, A., Vaz, G. M. F., Urquhart, A. J., Senge, M. O., Richard, D. J., O'Byrne, K. J., & Davies, A. M. (2016) · Assay and Drug Development Technologies · doi:10.1089/adt.2015.670 · Cited by 126
Towards personalized computational oncology: from spatial models of tumour spheroids, to organoids, to tissues
Karolak, A. M., Markov, D. A., McCawley, L. J., & Rejniak, K. A. (2018) · Journal of The Royal Society Interface · doi:10.1098/rsif.2017.0703 · Cited by 121
Human biomimetic liver microphysiology systems in drug development and precision medicine
Gough, A., Soto-Gutierrez, A., Vernetti, L., Ebrahimkhani, M. R., Stern, A. M., & Taylor, D. L. (2021) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-020-00386-1 · Cited by 117
Combinatorial therapy in tumor microenvironment: Where do we stand?
Kumari, S., Advani, D., Sharma, S., Ambasta, R. K., & Kumar, P. (2021) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2021.188585 · Cited by 121
Organoids and the genetically encoded self‐assembly of embryonic stem cells
Turner, D. A., Baillie‐Johnson, P., & Arias, A. M. (2015) · BioEssays · doi:10.1002/bies.201500111 · Cited by 122
Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
Jardé, T., Lloyd‐Lewis, B., Thomas, M., Kendrick, H. D., Melchor, L., Bougaret, L., Watson, P., Ewan, K., Smalley, M. J., & Dale, T. C. (2016) · Nature Communications · doi:10.1038/ncomms13207 · Cited by 120
On human pluripotent stem cell control: The rise of 3D bioengineering and mechanobiology
Shao, Y., Sang, J., & Fu, J. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.01.078 · Cited by 117
Microtubules in 3D cell motility
Bouchet, B. P., & Akhmanova, A. S. (2017) · Journal of Cell Science · doi:10.1242/jcs.189431 · Cited by 119
The molecular basis of mammary gland development and epithelial differentiation
Slepicka, P. F., Somasundara, A. V. H., & dos Santos, C. O. (2020) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2020.09.014 · Cited by 120
Glioblastoma stem cell differentiation into endothelial cells evidenced through live-cell imaging
Mei, X., Chen, Y.-S., Chen, F., Xi, S., & Chen, Z.-P. (2017) · Neuro-Oncology · doi:10.1093/neuonc/nox016 · Cited by 110
Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research
Sun, L., Yang, H., Wang, Y., Zhang, X., Jin, B., Xie, F., Jin, Y., Pang, Y., Zhao, H., Lu, X., et al. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.00878 · Cited by 109
Gastric organoids—an in vitro model system for the study of gastric development and road to personalized medicine
Seidlitz, T., Koo, B., & Stange, D. E. (2020) · Cell Death and Differentiation · doi:10.1038/s41418-020-00662-2 · Cited by 112
Stem Cells: A Renaissance in Human Biology Research
Wu, J., & Belmonte, J. C. I. (2016) · Cell · doi:10.1016/j.cell.2016.05.043 · Cited by 111
Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer
Zhao, Y., Li, S., Zhu, L., Huang, M., Xie, Y., Song, X., Chen, Z., Lau, H. C.-H., Sung, J. J.-Y., Xu, L., et al. (2024) · Cell Reports Medicine · doi:10.1016/j.xcrm.2024.101627 · Cited by 113
Three dimensional in vitro models of cancer: Bioprinting multilineage glioblastoma models
Hermida, M. A., Kumar, J. D., Schwarz, D., Laverty, K. G., Bartolo, A. D., Ardron, M., Bogomolnijs, M., Clavreul, A., Brennan, P. M., Wiegand, U. K., et al. (2019) · Advances in Biological Regulation · doi:10.1016/j.jbior.2019.100658 · Cited by 106
Three-Dimensional In Vitro Cell Culture Models for Efficient Drug Discovery: Progress So Far and Future Prospects
Badr-Eldin, S. M., Aldawsari, H. M., Kotta, S., Deb, P. K., & Venugopala, K. N. (2022) · Pharmaceuticals · doi:10.3390/ph15080926 · Cited by 108
Three‐Dimensional Models of the Human Brain Development and Diseases
Jorfi, M., D’Avanzo, C., Kim, D. Y., & Irimia, D. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700723 · Cited by 106
Engineered Microsystems for Spheroid and Organoid Studies
Kang, S., Kim, D., Lee, J., Takayama, S., & Park, J. Y. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202001284 · Cited by 100
Bioengineering-inspired three-dimensional culture systems: Organoids to create tumor microenvironment
Saglam‐Metiner, P., İz, S. G., & Avcı, Ç. B. (2018) · Gene · doi:10.1016/j.gene.2018.11.058 · Cited by 100
The Rise of Physiologic Media
Cantor, J. R. (2019) · Trends in Cell Biology · doi:10.1016/j.tcb.2019.08.009 · Cited by 107
Patterned cell and matrix dynamics in branching morphogenesis
Wang, S., Sekiguchi, R., Daley, W. P., & Yamada, K. M. (2017) · The Journal of Cell Biology · doi:10.1083/jcb.201610048 · Cited by 103
Organoids of the female reproductive tract
Chumduri, C., & Turco, M. Y. (2021) · Journal of Molecular Medicine · doi:10.1007/s00109-020-02028-0 · Cited by 110
Reviewing the metabolome coverage provided by LC-MS: Focus on sample preparation and chromatography-A tutorial
Roca, M., Alcoriza‐Balaguer, M. I., García‐Cañaveras, J. C., & Lahoz, A. (2020) · Analytica Chimica Acta · doi:10.1016/j.aca.2020.12.025 · Cited by 102
Primary Mammary Organoid Model of Lactation and Involution
Sumbal, J., Chiche, A., Charifou, E., Koledova, Z. S., & Li, H. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00068 · Cited by 101
Development and application of human adult stem or progenitor cell organoids
Rookmaaker, M. B., Schutgens, F., Verhaar, M. C., & Clevers, H. C. (2015) · Nature Reviews Nephrology · doi:10.1038/nrneph.2015.118 · Cited by 91
The potential of organoids in urological cancer research
Wang, S., Gao, D., & Chen, Y. (2017) · Nature Reviews Urology · doi:10.1038/nrurol.2017.65 · Cited by 87
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer
Bajikar, S. S., Wang, C., Borten, M. A., Pereira, E. J., Atkins, K. A., & Janes, K. A. (2017) · Developmental Cell · doi:10.1016/j.devcel.2017.10.027 · Cited by 87
Human brain metastatic stroma attracts breast cancer cells via chemokines CXCL16 and CXCL12
Chung, B., Esmaeili, A. A., Gopalakrishna-Pillai, S., Murad, J. P., Andersen, E. S., Reddy, N. K., Srinivasan, G., Armstrong, B., Chu, C. T., Kim, Y., et al. (2017) · npj Breast Cancer · doi:10.1038/s41523-017-0008-8 · Cited by 88
Pattern of Invasion in Human Pancreatic Cancer Organoids Is Associated with Loss of SMAD4 and Clinical Outcome
Huang, W., Navarro-Serer, B., Jeong, Y. J., Chianchiano, P. E., Xia, L., Luchini, C., Veronese, N., Dowiak, C., Ng, T., Trujillo, M. A. M., et al. (2020) · Cancer Research · doi:10.1158/0008-5472.can-19-1523 · Cited by 91
Self-citation
Organoids as Models for Neoplastic Transformation
Neal, J. T., & Kuo, C. J. (2016) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathol-012615-044249 · Cited by 85
A novel organ-chip system emulates three-dimensional architecture of the human epithelia and the mechanical forces acting on it
Varone, A., Nguyen, J. K., Leng, L., Barrile, R., Sliz, J. D., Lucchesi, C., Wen, N., Gravanis, A., Hamilton, G. A., Karalis, K., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120957 · Cited by 83
Bioprinting Methods for Fabricating In Vitro Tubular Blood Vessel Models
Kim, S. J., Kim, M.-G., Kim, J., Jeon, J. S., Park, J., & Yi, H. (2023) · Cyborg and Bionic Systems · doi:10.34133/cbsystems.0043 · Cited by 81
The current landscape of 3D in vitro tumor models: what cancer hallmarks are accessible for drug discovery?
Rodenhizer, D., Dean, T., D'Arcangelo, E., & McGuigan, A. P. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701174 · Cited by 80
Conditional Reprogramming for Patient-Derived Cancer Models and Next-Generation Living Biobanks
Palechor-Ceron, N., Krawczyk, E., Dakic, A., Simić, V., Yuan, H., Blancato, J. K., Wang, W., Hubbard, F. A., Zheng, Y., Dan, H., et al. (2019) · Cells · doi:10.3390/cells8111327 · Cited by 80
Scaffold‐Free Liver‐On‐A‐Chip with Multiscale Organotypic Cultures
Weng, Y., Chang, S., Shih, M., Tseng, S., & Lai, C. (2017) · Advanced Materials · doi:10.1002/adma.201701545 · Cited by 81
3D Cell Culture: An Introduction
Koledova, Z. S. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7021-6_1 · Cited by 79
Native-mimicking in vitro microenvironment: an elusive and seductive future for tumor modeling and tissue engineering
Rijal, G., & Li, W. (2018) · Journal of Biological Engineering · doi:10.1186/s13036-018-0114-7 · Cited by 80
Distinctive genomic signature of neural and intestinal organoids from familial Parkinson's disease patient‐derived induced pluripotent stem cells
Son, M., Sim, H., Son, Y., Jung, K. B., Lee, M. ‐., Oh, J., Chung, S. ‐., Jung, C., & Kim, J. (2017) · Neuropathology and Applied Neurobiology · doi:10.1111/nan.12396 · Cited by 78
Drug Discovery Goes Three-Dimensional: Goodbye to Flat High-Throughput Screening?
Eglen, R. M., & Randle, D. H. (2015) · Assay and Drug Development Technologies · doi:10.1089/adt.2015.647 · Cited by 79
A 3D co-culture microtissue model of the human placenta for nanotoxicity assessment
Muoth, C., Wichser, A., Monopoli, M. P., Correia, M., Ehrlich, N., Loeschner, K., Gallud, A., Kucki, M., Diener, L., Manser, P., et al. (2016) · Nanoscale · doi:10.1039/c6nr06749b · Cited by 79
Stem Cell-Based Therapies for Liver Diseases: An Overview and Update
Wang, J., Sun, M., Liu, W., Li, Y., & Li, M. (2019) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-019-00178-y · Cited by 77
Optical coherence microscopy in 1700 nm spectral band for high-resolution label-free deep-tissue imaging
Yamanaka, M., Teranishi, T., Kawagoe, H., & Nishizawa, N. (2016) · Scientific Reports · doi:10.1038/srep31715 · Cited by 77
3D high throughput screening and profiling of embryoid bodies in thermoformed microwell plates
Vrij, E., Espinoza, S., Heilig, M. A., Kolew, A., Schneider, M., van Blitterswijk, C., Truckenmüller, R. K., & Rivron, N. C. (2016) · Lab on a Chip · doi:10.1039/c5lc01499a · Cited by 75
An In Vitro System for Evaluating Molecular Targeted Drugs Using Lung Patient-Derived Tumor Organoids
Takahashi, N., Hoshi, H., Higa, A., Hiyama, G., Tamura, H., Ogawa, M., Takagi, K., Goda, K., Okabe, N., Muto, S., et al. (2019) · Cells · doi:10.3390/cells8050481 · Cited by 75
Neural Lineage Differentiation From Pluripotent Stem Cells to Mimic Human Brain Tissues
Hong, Y. J., & Tae, J. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00400 · Cited by 78
Multicomponent hydrogels for the formation of vascularized bone-like constructs in vitro
Derkuş, B., Okesola, B. O., Barrett, D. W., D’Este, M., Chowdhury, T. T., Eglin, D., & Mata, Á. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.03.025 · Cited by 74
Engineered tissues and strategies to overcome challenges in drug development
Khalil, A. S., Jaenisch, R., & Mooney, D. J. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.09.012 · Cited by 76

Authors: Shamir, E. R., & Ewald, A. J.
Affiliations: Johns Hopkins University (US)
Corresponding author: Ewald, Andrew J.
ORCID: Shamir, Eliah R.
Ewald, Andrew J.
Venue: Nature Reviews Molecular Cell Biology
Publisher: Springer Science and Business Media LLC
Published: 2014-09-17
Volume: 15
Issue: 10
Pages: 647-664
ISSN: 1471-0072 (ISSN-L)
1471-0080
DOI: 10.1038/nrm3873
PMC: PMC4352326
OpenAlex: W2052700237
Cited by: 719
References: 199
Citations by year: 2026: 19 · 2025: 40 · 2024: 41 · 2023: 51 · 2022: 67 · 2021: 71 · 2020: 95 · 2019: 64 · 2018: 71 · 2017: 90 · 2016: 74 · 2015: 36
FWCI: 5.73
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Reviews Molecular Cell Biology (publishedVersion)
PubMed (publishedVersion)
Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Cancer Institute (U01CA155758, P30CA006973)
National Institute of General Medical Sciences (T32GM007309)
Topics: Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Keywords: organotypic culture, 3D cell culture, epithelial morphogenesis, micro-environmental factors, organ models, tissue engineering, patient-derived cells, disease models, therapeutic testing
MeSH terms: Animals, Epithelial Cells, Epithelium, Gene Expression Regulation, Mammals, Morphogenesis, Organ Culture Techniques, Cell Culture Techniques, Cell Proliferation, Cellular Microenvironment

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Three-dimensional organotypic culture: experimental models of mammalian biology and disease

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks