The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
One SNARE complex is sufficient for membrane fusion
Open accessAbstract
In eukaryotes, most intracellular membrane fusion reactions are mediated by the interaction of SNARE proteins that are present in both fusing membranes. However, the minimal number of SNARE complexes needed for membrane fusion is not known. Here we show unambiguously that one SNARE complex is sufficient for membrane fusion. We performed controlled in vitro Förster resonance energy transfer (FRET) experiments and found that liposomes bearing only a single SNARE molecule are still capable of fusion with other liposomes or with purified synaptic vesicles. Furthermore, we demonstrated that multiple SNARE complexes do not act cooperatively, showing that synergy between several SNARE complexes is not needed for membrane fusion. Our findings shed new light on the mechanism of SNARE-mediated membrane fusion and call for a revision of current views of fusion events such as the fast release of neurotransmitters.
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Cites
SNAREs — engines for membrane fusion
Jahn, R., & Scheller, R. H. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2002 · Cited by 2,635
Self-citationSNAREpins: Minimal Machinery for Membrane Fusion
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Molecular anatomy of a trafficking organelle
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Self-citationStructure of Fully Hydrated Fluid Phase Lipid Bilayers with Monounsaturated Chains
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Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster
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Stalk Model of Membrane Fusion: Solution of Energy Crisis
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N- to C-Terminal SNARE Complex Assembly Promotes Rapid Membrane Fusion
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Transmembrane Segments of Syntaxin Line the Fusion Pore of Ca 2 +-Triggered Exocytosis
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Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy
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Synaptotagmin-Mediated Bending of the Target Membrane Is a Critical Step in Ca2+-Regulated Fusion
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Cited by
Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle
Südhof, T. C. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.022 · Cited by 1,197
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins
Wilhelm, B. G., Mandad, S., Truckenbrodt, S., Kröhnert, K., Schäfer, C., Rammner, B., Koo, S. J., Claßen, G. A., Krauss, M., Haucke, V., et al. (2014) · Science · doi:10.1126/science.1252884 · Cited by 770
Molecular Mechanisms for Synchronous, Asynchronous, and Spontaneous Neurotransmitter Release
Kaeser, P. S., & Regehr, W. G. (2014) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021113-170338 · Cited by 447
The Synaptic Vesicle Release Machinery
Rizo, J., & Xu, J. (2015) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-060414-034057 · Cited by 339
LRRK2 Controls an EndoA Phosphorylation Cycle in Synaptic Endocytosis
Matta, S., Van Kolen, K., da Cunha, R., van den Bogaart, G., Mandemakers, W., Miskiewicz, K., De Bock, P.-J., Morais, V. A., Vilain, S., Haddad, D., et al. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.022 · Cited by 337
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Metadata
- Authors
- van den Bogaart, G., Holt, M. G., Bunt, G., Riedel, D., Wouters, F. S., & Jahn, R.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2010-02-07
- Volume
- 17
- Issue
- 3
- Pages
- 358-364
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W1968306435
- Cited by
- 289
- References
- 37
- FWCI
- 12.15
- Type
- journal article
- Language
- en
- Open access
- green
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- van den Bogaart, G., Holt, M. G., Bunt, G., Riedel, D., Wouters, F. S., & Jahn, R. (2010). One SNARE complex is sufficient for membrane fusion. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1748
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