Cited by 108Infant Nutrition and HealthProbiotics and Fermented FoodsGut microbiota and health

Bifidobacterium longum subsp. infantis in experimental necrotizing enterocolitis: alterations in inflammation, innate immune response, and the microbiota

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Underwood, Mark A., Arriola, Jennifer, Gerber, Colin W., Kaveti, Ashwini, Kalanetra, Karen M., Kananurak, Anchasa, Bevins, Charles L., Mills, David A. +1 more·2014·Pediatric Research

Abstract

Background Probiotics decrease the risk of necrotizing enterocolitis (NEC). We sought to determine the impact of Bifidobacterium longum subsp. infantis (B. infantis) in the established rat model of NEC.Methods Rat pups delivered 1 d prior to term gestation were assigned to one of three groups: dam fed (DF), formula fed (FF), or fed with formula supplemented with 5 × 10(6) CFU B. infantis per day (FF+Binf). Experimental pups were exposed to hypoxia and cold stress. Ileal tissue was examined for pathology and expression of inflammatory mediators, antimicrobial peptides, and goblet-cell products. Ceca were assessed for bacterial composition by analysis of the 16S rRNA sequence.Results Administration of B. infantis significantly reduced the incidence of NEC, decreased expression of Il6, Cxcl1, Tnfa, Il23, and iNOS, and decreased expression of the antimicrobial peptides Reg3b and Reg3g. There was significant microbial heterogeneity both within groups and between experiments. The cecal microbiota was not significantly different between the FF and FF+Binf groups. Bifidobacteria were not detected in the cecum in significant numbers.Conclusion In the rat model, the inflammation associated with NEC was attenuated by administration of probiotic B. infantis. Dysbiosis was highly variable, precluding determination of the precise role of the microbiota in experimental NEC.

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Authors
Underwood, M. A., Arriola, J., Gerber, C. W., Kaveti, A., Kalanetra, K. M., Kananurak, A., Bevins, C. L., Mills, D. A., & Dvorak, B.
Affiliations
  • University of California Davis Medical Center (US)
  • University of Arizona (US)
  • University of California, Davis (US)
Corresponding author
Underwood, Mark A.
Venue
Pediatric Research
Published
2014-07-07
Volume
76
Issue
4
Pages
326-333
ISSN
  • 0031-3998 (ISSN-L)
  • 1530-0447
OpenAlex
W2028376726
Cited by
107
References
46
Citations by year
2025: 6 · 2024: 8 · 2023: 12 · 2022: 14 · 2021: 9 · 2020: 10 · 2019: 8 · 2018: 9 · 2017: 14 · 2016: 10 · 2015: 4 · 2014: 3
FWCI
9.63
Type
journal article
Language
en
Open access
Locations
  • Pediatric Research (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • Mead Johnson Nutrition
  • National Institutes of Health
  • University of California, Davis (AT007079)
  • National Institute of Allergy and Infectious Diseases (R01AI032738, AI-32738, R37AI032738)
  • National Center for Complementary and Integrative Health (R01AT007079, AT007079)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-039657, HD059127, R01HD059127, R01HD039657)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Bifidobacterium longum subsp. infantis, necrotizing enterocolitis, probiotics, gut microbiota, inflammation, innate immune response, antimicrobial peptides, Reg3b, Reg3g, hypoxia, cold stress, cecal microbiota, dysbiosis, neonatal rat model
MeSH terms
Animals, Bifidobacterium, Cecum, Immunity, Innate, Inflammation, RNA, Ribosomal, 16S, Enterocolitis, Necrotizing, Rats, Microbiota

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