Intermittent cafeteria diet identifies fecal microbiome changes as a predictor of spatial recognition memory impairment in female rats
Open accessAbstract
Excessive consumption of diets high in saturated fat and sugar impairs short-term spatial recognition memory in both humans and rodents. Several studies have identified associations between the observed behavioral phenotype and diet-induced changes in adiposity, hippocampal gene expression of inflammatory and blood-brain barrier-related markers, and gut microbiome composition. However, the causal role of such variables in producing cognitive impairments remains unclear. As intermittent cafeteria diet access produces an intermediate phenotype, we contrasted continuous and intermittent diet access to identify specific changes in hippocampal gene expression and microbial species that underlie the cognitive impairment observed in rats fed continuous cafeteria diet. Female adult rats were fed either regular chow, continuous cafeteria diet, or intermittent cafeteria diet cycles (4 days regular chow and 3 days cafeteria) for 7 weeks (12 rats per group). Any cafeteria diet exposure affected metabolic health, hippocampal gene expression, and gut microbiota, but only continuous access impaired short-term spatial recognition memory. Multiple regression identified an operational taxonomic unit, from species Muribaculum intestinale, as a significant predictor of performance in the novel place recognition task. Thus, contrasting intermittent and continuous cafeteria diet exposure allowed us to identify specific changes in microbial species abundance and growth as potential underlying mechanisms relevant to diet-induced cognitive impairment.
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Differences in Coprobacter elicited by varying lengths of exposure to the cafeteria diet was identified as a predictor of performances in spatial recognition memory.
“For example, differences in Coprobacter elicited by varying lengths of exposure to the cafeteria diet was identified as a predictor of performances in spatial recognition memory (322).”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [322]
In rats, a cafeteria diet decreased α-diversity and impaired spatial recognition in a novel place recognition task, whereas a cycle cafeteria diet did not.
“↓ Spatial recognition (novel | place recognition task) in Caf | but not cycle diet”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [322]
In rats, performance in a novel place recognition task was a significant predictor of global microbiome composition.
“Performance in novel place | recognition task was | significant predictor of global | microbiome composition”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [322]
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Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health
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- Authors
- Leigh, S.-J., Kaakoush, N. O., Bertoldo, M. J., Westbrook, R. F., & Morris, M. J.
- Affiliations
- UNSW Sydney (AU)
- Corresponding author
- Morris, Margaret J.
- ORCID
- Venue
- Translational Psychiatry
- Published
- 2020-01-27
- Volume
- 10
- Issue
- 1
- Pages
- 36-36
- ISSN
- 2158-3188 (ISSN-L)
- PMC
- PMC7026185
- OpenAlex
- W3002859174
- Cited by
- 45
- References
- 65
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 6 · 2023: 4 · 2022: 10 · 2021: 12 · 2020: 6
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Translational Psychiatry (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Department of Health | National Health and Medical Research Council (1126929)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Keywords
- high-fat high-sugar diet, spatial recognition memory, short-term memory impairment, gut microbiota, Muribaculum intestinale, hippocampal gene expression, female rats, metabolic health
- MeSH terms
- Animals, Diet, Female, Rats, Sprague-Dawley, Bacteroidetes, Rats, Diet, High-Fat, Microbiota
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