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Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

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Mohammad-Beigi, Hossein, Hayashi, Yuya, Zeuthen, Christina Moeslund, Eskandari, Hoda, Scavenius, Carsten, Juul-Madsen, Kristian, Vorup-Jensen, Thomas, Enghild, Jan J., Sutherland, Duncan S.·2020·Nature Communications
DOI 10.1038/s41467-020-18237-7

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Abstract

The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

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Metadata

Authors
Mohammad-Beigi, H., Hayashi, Y., Zeuthen, C. M., Eskandari, H., Scavenius, C., Juul-Madsen, K., Vorup-Jensen, T., Enghild, J. J., & Sutherland, D. S.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2020-09-10
Volume
11
Issue
1
Pages
4535-4535
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-020-18237-7
OpenAlex
W3083833107
Cited by
218
References
5
FWCI
7.02
Type
journal article
Language
en
Open access
gold
Licence

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CoChat. (2026). Citation check: Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. https://app.cochat.ai/papers/10.1038/s41467-020-18237-7
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Mohammad-Beigi, H., Hayashi, Y., Zeuthen, C. M., Eskandari, H., Scavenius, C., Juul-Madsen, K., Vorup-Jensen, T., Enghild, J. J., & Sutherland, D. S. (2020). Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. Nature Communications. https://doi.org/10.1038/s41467-020-18237-7

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