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Cited by 70Antifungal resistance and susceptibilityFungal and yeast genetics researchFungal Infections and Studies

Adaptative survival of Aspergillus fumigatus to echinocandins arises from cell wall remodeling beyond β−1,3-glucan synthesis inhibition

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Dickwella Widanage, Malitha C., Gautam, Isha, Sarkar, Daipayan, Mentink-Vigier, Frederic, Vermaas, Josh V., Ding, Shi-You, Lipton, Andrew S., Fontaine, Thierry +3 more·2024·Nature Communications
DOI 10.1038/s41467-024-50799-8PMC PMC11291495

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Abstract

Antifungal echinocandins inhibit the biosynthesis of β−1,3-glucan, a major and essential polysaccharide component of the fungal cell wall. However, the efficacy of echinocandins against the pathogen Aspergillus fumigatus is limited. Here, we use solid-state nuclear magnetic resonance (ssNMR) and other techniques to show that echinocandins induce dynamic changes in the assembly of mobile and rigid polymers within the A. fumigatus cell wall. The reduction of β−1,3-glucan induced by echinocandins is accompanied by a concurrent increase in levels of chitin, chitosan, and highly polymorphic α−1,3-glucans, whose physical association with chitin maintains cell wall integrity and modulates water permeability. The rearrangement of the macromolecular network is dynamic and controls the permeability and circulation of the drug throughout the cell wall. Thus, our results indicate that echinocandin treatment triggers compensatory rearrangements in the cell wall that may help A. fumigatus to tolerate the drugs’ antifungal effects.

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  • Reduced water permeability is a common response previously observed in several Aspergillus species under stress.

    “Both C. albicans and C. auris AR386 exhibit reduced water permeability (Fig. 4a), a common response previously also observed in several Aspergillus species under stress50,53.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [50]

    The Results section, “Reduced water retention and polymer dynamics” (page unavailable), reports reduced water accessibility in A. fumigatus cell walls after caspofungin treatment and suggests this should reduce cell-wall permeability. It does not establish reduced water permeability as a common response across several Aspergillus species or under stress generally, and permeability was inferred rather than directly measured. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • The balance between rigid structural components and mobile, water-accessible regions allows fungi to fine-tune cell wall permeability, aiding adaptive survival.

    “Recent observations suggest that the balance between rigid structural components and mobile, water-accessible regions allows fungi to fine-tune cell wall permeability, aiding adaptive survival50,65.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [50]

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Cited by

Sorted by citations

Authors
Dickwella Widanage, M. C., Gautam, I., Sarkar, D., Mentink-Vigier, F., Vermaas, J. V., Ding, S.-Y., Lipton, A. S., Fontaine, T., Latgé, J.-P., Wang, P., & Wang, T.
Affiliations
  • Michigan State University (US)
  • Pacific Northwest National Laboratory (US)
  • Institut Pasteur (FR)
  • University of Crete (GR)
  • Louisiana State University Health Sciences Center New Orleans (US)
Corresponding author
Wang, Tuo
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-07-31
Volume
15
Issue
1
Pages
6382-6382
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-024-50799-8
PMC
PMC11291495
OpenAlex
W4401184074
Cited by
70
References
11
Citations by year
2026: 34 · 2025: 31 · 2024: 5
FWCI
24.07
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • U.S. Department of Health & Human Services | National Institutes of Health (AI173270, R35GM155317)
  • U.S. Department of Energy (DE-SC0019072)
  • National Science Foundation (2321398)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Aspergillus fumigatus, echinocandins, antifungal resistance, β-1,3-glucan, chitin, chitosan, α-1,3-glucan, cell wall integrity, water permeability, drug permeation, antifungal tolerance
MeSH terms
Antifungal Agents, Aspergillus fumigatus, Cell Wall, Chitin, Glucans, Microbial Sensitivity Tests, Magnetic Resonance Spectroscopy, beta-Glucans, Chitosan, Echinocandins

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