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Prime genome editing in rice and wheat
Abstract
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
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“Prime editing allows for all types of desired base substitutions, small insertions up to 44 bp and deletions up to 88 bp at selected target sites with the use of CRSIPR/Cas9 nickase-reverse transcriptase fusions (Anzalone et al., 2019;Lin et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lin et al., 2020
In the citing paper
“Plant prime-editing systems have been used in rice and wheat, but not in barley (Lin et al., 2020;Xu et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lin et al., 2020
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Metadata
- Authors
- Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., Liu, D. R., & Gao, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Broad Institute (US)
- Corresponding author
- Gao, Caixia
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2020-03-16
- Volume
- 38
- Issue
- 5
- Pages
- 582-585
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W3012016445
- Semantic Scholar
- 8cfc62e789552485d639d0542c1caaaf730db03d
- Cited by
- 873
- References
- 23
- Citations by year
- 2026: 105 · 2025: 118 · 2024: 106 · 2023: 135 · 2022: 146 · 2021: 175 · 2020: 86
- FWCI
- 44.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Human Genome Research Institute (RM1HG009490)
- National Institute of General Medical Sciences (R35GM118062)
- National Institute of Allergy and Infectious Diseases (U01AI142756)
- National Institute of Biomedical Imaging and Bioengineering (R01EB022376)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- prime editing, CRISPR/Cas9 nickase, reverse transcriptase, pegRNA, plant genome editing, rice, wheat, protoplast transformation, point mutations, indels, codon optimization, promoter optimization, plant regeneration
- MeSH terms
- Gene Editing, Deoxyribonuclease I, Oryza, Triticum, Genome, Plant, CRISPR-Cas Systems
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