Cited by 110Memory and Neural MechanismsSingle-cell and spatial transcriptomicsNeuroscience and Neuropharmacology Research

Neuronal types in the mouse amygdala and their transcriptional response to fear conditioning

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Hochgerner, Hannah, Singh, Shelly, Tibi, Muhammad, Lin, Zhige, Skarbianskis, Niv, Admati, Inbal, Ophir, Osnat, Reinhardt, Nuphar +3 more·2023·Nature Neuroscience

Abstract

The amygdala is a brain region primarily associated with emotional response. The use of genetic markers and single-cell transcriptomics can provide insights into behavior-associated cell state changes. Here we present a detailed cell-type taxonomy of the adult mouse amygdala during fear learning and memory consolidation. We perform single-cell RNA sequencing on naïve and fear-conditioned mice, identify 130 neuronal cell types and validate their spatial distributions. A subset of all neuronal types is transcriptionally responsive to fear learning and memory retrieval. The activated engram cells upregulate activity-response genes and coordinate the expression of genes associated with neurite outgrowth, synaptic signaling, plasticity and development. We identify known and previously undescribed candidate genes responsive to fear learning. Our molecular atlas may be used to generate hypotheses to unveil the neuron types and neural circuits regulating the emotional component of learning and memory.

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  1. [77]Ester, M., Kriegel, H.-P., Sander, J. & Xu, X. A density-based algorithm for discovering clusters in large spatial databases with noise. Proceedings of the Second International Conference on Knowledge Discovery and Data Mining (KDD'96) pp. 226–231 (AAAI Press, 1996).

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Authors
Hochgerner, H., Singh, S., Tibi, M., Lin, Z., Skarbianskis, N., Admati, I., Ophir, O., Reinhardt, N., Netser, S., Wagner, S., & Zeisel, A.
Affiliations
  • Technion – Israel Institute of Technology (IL)
  • University of Haifa (IL)
Corresponding author
Zeisel, Amit
Venue
Nature Neuroscience
Published
2023-10-26
Volume
26
Issue
12
Pages
2237-2249
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W4387965083
Cited by
110
References
78
Citations by year
2026: 34 · 2025: 48 · 2024: 27 · 2023: 1
FWCI
16.47
Type
journal article
Language
en
Open access
Locations
  • Nature Neuroscience (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Israel Science Foundation (2028912)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
amygdala, neuronal cell types, fear conditioning, fear memory consolidation, memory retrieval, engram cells, single-cell transcriptomics, molecular atlas, synaptic plasticity, neurite outgrowth
MeSH terms
Amygdala, Animals, Fear, Memory, Neuronal Plasticity, Neurons, Mice

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