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Cited by 228Genomics and Phylogenetic StudiesForensic and Genetic ResearchEnvironmental DNA in Biodiversity Studies

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA

Gansauge, Marie-Theres, Aximu-Petri, Ayinuer, Nagel, Sarah, Meyer, Matthias·2020·Nature Protocols
DOI 10.1038/s41596-020-0338-0
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Abstract

It has been shown that highly fragmented DNA is most efficiently converted into DNA libraries for sequencing if both strands of the DNA fragments are processed independently. We present an updated protocol for library preparation from single-stranded DNA, which is based on the splinted ligation of an adapter oligonucleotide to the 3' ends of single DNA strands, the synthesis of a complementary strand using a DNA polymerase and the addition of a 5' adapter via blunt-end ligation. The efficiency of library preparation is determined individually for each sample using a spike-in oligonucleotide. The whole workflow, including library preparation, quantification and amplification, requires two work days for up to 16 libraries. Alternatively, we provide documentation and electronic protocols enabling automated library preparation of 96 samples in parallel on a Bravo NGS Workstation (Agilent Technologies). After library preparation, molecules with uninformative short inserts (shorter than ~30-35 base pairs) can be removed by polyacrylamide gel electrophoresis if desired.

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Crossref · 10.1038/s41596-020-0338-0

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  • Large-scale projects can benefit from nascent automation procedures for preparation of DNA libraries and their capture.

    “Large-scale projects can benefit from nascent automation procedures both for the preparation of DNA libraries and their capture 49,126,144 .”

    From Ancient DNA analysis (2021) · cites this paper as [144]

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Research works printed but not linked: 36

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Metadata

Authors
Gansauge, M.-T., Aximu-Petri, A., Nagel, S., & Meyer, M.
Affiliations
Max Planck Institute for Evolutionary Anthropology (DE)
Corresponding author
  • Gansauge, Marie-Theres
  • Meyer, Matthias
ORCID
Venue
Nature Protocols
Publisher
Springer Science and Business Media LLC
Published
2020-07-01
Volume
15
Issue
8
Pages
2279-2300
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
DOI
10.1038/s41596-020-0338-0
OpenAlex
W3039475142
Cited by
228
References
11
Citations by year
2026: 49 · 2025: 53 · 2024: 27 · 2023: 35 · 2022: 32 · 2021: 25 · 2020: 7
FWCI
7.08
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Nature Protocols (publishedVersion)
  • PubMed (publishedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • Max Planck Society
  • Max-Planck-Gesellschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
  • Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
Keywords
single-stranded DNA library preparation, ancient DNA, blunt-end ligation, automated library preparation
MeSH terms
DNA, Ancient, Automation, Base Sequence, DNA, Single-Stranded, Gene Library, Sequence Analysis, DNA

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