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Cited by 165Alzheimer's disease research and treatmentsNeuroinflammation and Neurodegeneration MechanismsDementia and Cognitive Impairment Research

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Comparative profiling of cortical gene expression in Alzheimer’s disease patients and mouse models demonstrates a link between amyloidosis and neuroinflammation

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Castillo, Erika, Leon, Julio, Mazzei, Guianfranco, Abolhassani, Nona, Haruyama, Naoki, Saito, Takashi, Saido, Takaomi, Hokama, Masaaki, Iwaki, Toru, Ohara, Tomoyuki, Ninomiya, Toshiharu, Kiyohara, Yutaka, Sakumi, Kunihiko, LaFerla, Frank M., Nakabeppu, Yusaku·2017·Scientific Reports
DOI 10.1038/s41598-017-17999-3PMC PMC5736730
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Abstract

Abstract Alzheimer’s disease (AD) is the most common form of dementia, characterized by accumulation of amyloid β (Aβ) and neurofibrillary tangles. Oxidative stress and inflammation are considered to play an important role in the development and progression of AD. However, the extent to which these events contribute to the Aβ pathologies remains unclear. We performed inter-species comparative gene expression profiling between AD patient brains and theAppNL-G-F/NL-G-Fand 3xTg-AD-H mouse models. Genes commonly altered inAppNL-G-F/NL-G-Fand human AD cortices correlated with the inflammatory response or immunological disease. Among them, expression of AD-related genes (C4a/C4b,Cd74,Ctss,Gfap,Nfe2l2,Phyhd1,S100b,Tf,Tgfbr2, andVim) was increased in theAppNL-G-F/NL-G-Fcortex as Aβ amyloidosis progressed with exacerbated gliosis, while genes commonly altered in the 3xTg-AD-H and human AD cortices correlated with neurological disease. TheAppNL-G-F/NL-G-Fcortex also had altered expression of genes (Abi3,Apoe,Bin2,Cd33,Ctsc,Dock2,Fcer1g,Frmd6,Hck,Inpp5D,Ly86,Plcg2,Trem2,Tyrobp) defined as risk factors for AD by genome-wide association study or identified as genetic nodes in late-onset AD. These results suggest a strong correlation between cortical Aβ amyloidosis and the neuroinflammatory response and provide a better understanding of the involvement of gender effects in the development of AD.

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Figure 1 Altered gene expression profiles in cortices of AD patients and AD mouse models. Lists of transcript clusters that exhibit significant alterations between AD patients and non-AD subjects or between AD mouse model and its control, obtained from microarray data (ANOVA: P < 0.05, fold change ≥ 1.2 or ≤−1.2), were subjected to hierarchical clustering analysis: (a) human AD temporal cortices, (b) human AD frontal cortices, (c) App NL-G-F/NL-G-F mouse cortices, (d) 3xTg-AD-H mouse cortices, compared with each control. Red columns indicate data from AD patients, App NL-G-F/NL-G-F and 3xTg-AD-H mice, blue columns represent data from each control. Levels of gene expression are shown in green (low) to red (high). (e) Venn diagram shows overlapping genes with significantly altered expression in each comparison (ANOVA: P < 0.05, log2 > 6.64, fold change ≥ 1.2 or ≤−1.2), between and among the four sets of comparisons. Total number of up- and downregulated genes in each group is shown in parentheses.
Figure 1 Altered gene expression profiles in cortices of AD patients and AD mouse models. Lists of transcript clusters that exhibit significant alterations between AD patients and non-AD subjects or between AD mouse model and its control, obtained from microarray data (ANOVA: P < 0.05, fold change ≥ 1.2 or ≤−1.2), were subjected to hierarchical clustering analysis: (a) human AD temporal cortices, (b) human AD frontal cortices, (c) App NL-G-F/NL-G-F mouse cortices, (d) 3xTg-AD-H mouse cortices, compared with each control. Red columns indicate data from AD patients, App NL-G-F/NL-G-F and 3xTg-AD-H mice, blue columns represent data from each control. Levels of gene expression are shown in green (low) to red (high). (e) Venn diagram shows overlapping genes with significantly altered expression in each comparison (ANOVA: P < 0.05, log2 > 6.64, fold change ≥ 1.2 or ≤−1.2), between and among the four sets of comparisons. Total number of up- and downregulated genes in each group is shown in parentheses.CC BY · PubMed Central
Figure 2 Biological functions of commonly altered genes in cortices of AD mouse models and AD patients. List of genes with commonly altered expression between the App NL-G-F/NL-G-F mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S8 (a), and between the 3xTg-AD-H mouse and human AD cortices (frontal and temporal) shown in Supplementary Tables S9 (b), were subjected to IPA Core Analysis. In each graph, black bars indicate the P-value (−log [P-value]); blue lines indicate the number of molecules categorised in each biological function. The red dashed line indicates the threshold for P-value (−log [P-value] = 1.3).
Figure 2 Biological functions of commonly altered genes in cortices of AD mouse models and AD patients. List of genes with commonly altered expression between the App NL-G-F/NL-G-F mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S8 (a), and between the 3xTg-AD-H mouse and human AD cortices (frontal and temporal) shown in Supplementary Tables S9 (b), were subjected to IPA Core Analysis. In each graph, black bars indicate the P-value (−log [P-value]); blue lines indicate the number of molecules categorised in each biological function. The red dashed line indicates the threshold for P-value (−log [P-value] = 1.3).CC BY · PubMed Central
Figure 3 Top networks of commonly altered genes in cortices of two AD mouse models and AD patients. (a) The top 3 networks of genes with commonly altered expression between the App NL-G-F/NL-G-F mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S8. Network 1 includes 17 upregulated genes and 1 downregulated gene. Network 2 includes 20 upregulated genes and 1 downregulated gene. Network 3 includes 14 upregulated genes in AD cortices. We included a dashed line to connect Phyhd1 and App in Network 2, according to our results and a previous report19. (b) The top network of genes with commonly altered expression between the 3xTg-AD-H mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S9. Network 1 includes 3 upregulated and 9 downregulated genes in AD cortices. Encoded molecules were placed in an appropriate subcellular compartment based on IPA, and “other” denotes unspecific or unknown localization. Solid lines indicate direct interactions and dashed lines indicate indirect interactions. Fold change is denoted as a green-white-red colour gradient, from green (downregulated) to red (upregulated).
Figure 3 Top networks of commonly altered genes in cortices of two AD mouse models and AD patients. (a) The top 3 networks of genes with commonly altered expression between the App NL-G-F/NL-G-F mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S8. Network 1 includes 17 upregulated genes and 1 downregulated gene. Network 2 includes 20 upregulated genes and 1 downregulated gene. Network 3 includes 14 upregulated genes in AD cortices. We included a dashed line to connect Phyhd1 and App in Network 2, according to our results and a previous report19. (b) The top network of genes with commonly altered expression between the 3xTg-AD-H mouse and human AD cortices (frontal and temporal) shown in Supplementary Table S9. Network 1 includes 3 upregulated and 9 downregulated genes in AD cortices. Encoded molecules were placed in an appropriate subcellular compartment based on IPA, and “other” denotes unspecific or unknown localization. Solid lines indicate direct interactions and dashed lines indicate indirect interactions. Fold change is denoted as a green-white-red colour gradient, from green (downregulated) to red (upregulated).CC BY · PubMed Central
Figure 4 Comparison of expression levels of 11 common genes with functions related to Alzheimer’s disease. (a) Comparison between AD and non-AD temporal cortices. AD (n = 8), non-AD (n = 10). (b) Comparison between AD and non-AD frontal cortices. AD (n = 13), non-AD (n = 17). (c) Comparison between the App NL-G-F/NL-G-F and wild-type (WT) cortices (n = 3). (d) Comparison between the 3xTg-AD-H and non-Tg cortices (n = 3). Bi-weight average signal (log2) of the 11 AD-related genes obtained from microarray analysis are shown with SEM in bar graphs. Red bars, AD patients or AD mouse models; blue bars, non-AD or control mice. One-way between-subject ANOVA analysis was performed; *P < 0.05; **P < 0.001; ***P < 0.0001. Ten genes (C4A/C4B, CD74, CTSS, GFAP, NFE2L2, PHYHD1, S100B, TF, TGFBR2 and VIM) were commonly upregulated in both human AD temporal and App NL-G-F/NL-G-F cortices.
Figure 4 Comparison of expression levels of 11 common genes with functions related to Alzheimer’s disease. (a) Comparison between AD and non-AD temporal cortices. AD (n = 8), non-AD (n = 10). (b) Comparison between AD and non-AD frontal cortices. AD (n = 13), non-AD (n = 17). (c) Comparison between the App NL-G-F/NL-G-F and wild-type (WT) cortices (n = 3). (d) Comparison between the 3xTg-AD-H and non-Tg cortices (n = 3). Bi-weight average signal (log2) of the 11 AD-related genes obtained from microarray analysis are shown with SEM in bar graphs. Red bars, AD patients or AD mouse models; blue bars, non-AD or control mice. One-way between-subject ANOVA analysis was performed; *P < 0.05; **P < 0.001; ***P < 0.0001. Ten genes (C4A/C4B, CD74, CTSS, GFAP, NFE2L2, PHYHD1, S100B, TF, TGFBR2 and VIM) were commonly upregulated in both human AD temporal and App NL-G-F/NL-G-F cortices.CC BY · PubMed Central
Figure 5 Effects of age and sex on expression levels of the 10 commonly upregulated genes related to Alzheimer’s disease in cortices of App NL-G-F/NL-G-F mice. Cortical RNA was isolated from male and female App NL-G-F/NL-G-F (red) and wild-type (WT, blue) mice (n = 3), at 5, 7 and 12 months of age, and subjected qRT-PCR. Expression levels, relative to Gapdh, of the 10 genes (C4b, Cd74, Ctss, Gfap, Nfe2l2, Phyhd1, S100b, Tf, Tgfbr2 and Vim) commonly upregulated in both App NL-G-F/NL-G-F mouse and human AD temporal cortices are shown. Data is expressed as mean value ± SEM of three independent mice performed in triplicate. Three-way ANOVA was performed and p-values for effects (sex, age and App genotype [App]) are shown. Detailed results of statistical analysis are shown in Supplementary Figs S6 and S7.
Figure 5 Effects of age and sex on expression levels of the 10 commonly upregulated genes related to Alzheimer’s disease in cortices of App NL-G-F/NL-G-F mice. Cortical RNA was isolated from male and female App NL-G-F/NL-G-F (red) and wild-type (WT, blue) mice (n = 3), at 5, 7 and 12 months of age, and subjected qRT-PCR. Expression levels, relative to Gapdh, of the 10 genes (C4b, Cd74, Ctss, Gfap, Nfe2l2, Phyhd1, S100b, Tf, Tgfbr2 and Vim) commonly upregulated in both App NL-G-F/NL-G-F mouse and human AD temporal cortices are shown. Data is expressed as mean value ± SEM of three independent mice performed in triplicate. Three-way ANOVA was performed and p-values for effects (sex, age and App genotype [App]) are shown. Detailed results of statistical analysis are shown in Supplementary Figs S6 and S7.CC BY · PubMed Central
Figure 6 Effects of age and sex on amyloid β deposition and glial activation in cortices of App NL-G-F/NL-G-F mice. Double-immunofluorescence microscopy for Aβ and GFAP (a,b), and Aβ and IBA1 (c,d). Coronal sections containing frontal (Bregma: −1.255 to −1.455) and temporal (Bregma: +1.845 to +2.045) cortices prepared from 5-, 7- and 12-month-old, male and female App NL-G-F/NL-G-F and wild-type (WT) mice, were subjected to double-immunofluorescence microscopy using mouse anti-human Aβ (green) and either anti-GFAP or rabbit anti-IBA1 antibodies (red). (a,c) Multiple z-stack images of 15 fields were tiled and stacked together using ZEN imaging software. Each immunoreactivity was measured, and means with SEM (n = 3) of the GFAP (a) and IBA1 (c) index are shown in the graphs on the right. Scale bar = 500 μm. Student’s t-test was performed between the two mouse lines at the given ages; *P < 0.05. (b,d) Magnified images for 5-month-old samples are shown. Nuclei were stained with DAPI (blue) in double-immunofluorescence images. Scale bar = 50 µm.
Figure 6 Effects of age and sex on amyloid β deposition and glial activation in cortices of App NL-G-F/NL-G-F mice. Double-immunofluorescence microscopy for Aβ and GFAP (a,b), and Aβ and IBA1 (c,d). Coronal sections containing frontal (Bregma: −1.255 to −1.455) and temporal (Bregma: +1.845 to +2.045) cortices prepared from 5-, 7- and 12-month-old, male and female App NL-G-F/NL-G-F and wild-type (WT) mice, were subjected to double-immunofluorescence microscopy using mouse anti-human Aβ (green) and either anti-GFAP or rabbit anti-IBA1 antibodies (red). (a,c) Multiple z-stack images of 15 fields were tiled and stacked together using ZEN imaging software. Each immunoreactivity was measured, and means with SEM (n = 3) of the GFAP (a) and IBA1 (c) index are shown in the graphs on the right. Scale bar = 500 μm. Student’s t-test was performed between the two mouse lines at the given ages; *P < 0.05. (b,d) Magnified images for 5-month-old samples are shown. Nuclei were stained with DAPI (blue) in double-immunofluorescence images. Scale bar = 50 µm.CC BY · PubMed Central

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  1. Pfaffi, M. W. Relative quantification, in real-time PCR (ed. Dorak M. T), Taylor & Francis Group, NY, pp63–82, 2006.

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Metadata

Authors
Castillo, E., Leon, J., Mazzei, G., Abolhassani, N., Haruyama, N., Saito, T., Saido, T., Hokama, M., Iwaki, T., Ohara, T., Ninomiya, T., Kiyohara, Y., Sakumi, K., LaFerla, F. M., & Nakabeppu, Y.
Affiliations
  • Kyushu University (JP)
  • RIKEN (JP)
  • Hisayama Research Institute For Lifestyle Diseases (JP)
  • University of California, Irvine (US)
Corresponding author
Nakabeppu, Yusaku
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-12-19
Volume
7
Issue
1
Pages
17762-17762
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-17999-3
PMC
PMC5736730
OpenAlex
W2771127066
Cited by
158
References
61
Citations by year
2026: 5 · 2025: 12 · 2024: 16 · 2023: 29 · 2022: 21 · 2021: 24 · 2020: 23 · 2019: 18 · 2018: 10
FWCI
10.31
Type
journal article
Language
en
Update notices
correction (2021-09-09)
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Kyushu University Institutional Repository (QIR) (Kyushu University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
Funders
  • Japan Agency for Medical Research and Development (H25-ninchisho-ippann-004)
  • Ministry of Health, Labour and Welfare (H20-ninchisho-ippan-004)
  • Fakultet Medicinskih Nauka, Univerziteta U Kragujevcu
  • Japan Society for the Promotion of Science (26290017, 22221004, 15K15085, 17H01391)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Keywords
Alzheimer's disease, neuroinflammation, cortical gene expression, gliosis, oxidative stress, sex differences
MeSH terms
Aged, Aged, 80 and over, Alzheimer Disease, Amyloidosis, Animals, Brain, Disease Models, Animal, Female, Gliosis, Humans, Inflammation, Male, Middle Aged, Gene Expression, Amyloid beta-Peptides, Gene Expression Profiling, Mice, Genome-Wide Association Study

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