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Cited by 51Xenotransplantation and immune responseCorneal Surgery and TreatmentsTissue Engineering and Regenerative Medicine

Finding an Optimal Corneal Xenograft Using Comparative Analysis of Corneal Matrix Proteins Across Species

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Sharifi, R., Yang, Y., Adibnia, Y., Dohlman, C. H., Chodosh, J., Gonzalez-Andrades, M.·2019·Scientific Reports
DOI 10.1038/s41598-018-38342-4PMC PMC6372616
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Abstract

Numerous animal species have been proposed as sources of corneal tissue for obtaining decellularized xenografts. The selection of an appropriate animal model must take into consideration the differences in the composition and structure of corneal proteins between humans and other animal species in order to minimize immune response and improve outcome of the xenotransplant. Here, we compared the amino-acid sequences of 16 proteins present in the corneal stromal matrix of 14 different animal species using Basic Local Alignment Search Tool, and calculated a similarity score compared to the respective human sequence. Primary amino acid structures, isoelectric point and grand average of hydropathy (GRAVY) values of the 7 most abundant proteins ( i.e . collagen α-1 (I), α-1 (VI), α-2 (I) and α-3 (VI), as well as decorin, lumican, and keratocan) were also extracted and compared to those of human. The pig had the highest similarity score (91.8%). All species showed a lower proline content compared to human. Isoelectric point of pig (7.1) was the closest to the human. Most species have higher GRAVY values compared to human except horse. Our results suggest that porcine cornea has a higher relative suitability for corneal transplantation into humans compared to other studied species.

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  • The application of many dECM scaffolds still encounters immunologic hurdles, hampering their translation into the clinic.

    “The application of many dECM scaffolds still encounters immunologic hurdles, hampering their translation into the clinic [33].”

    From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [33]

    The Introduction (page unavailable) says decellularization of xenogeneic corneas can leave antigenic components that still elicit an immune response. The Results and Discussion (page unavailable) likewise identifies antigenicity as an obstacle to successful corneal xenotransplantation. The paper supports the immunologic hurdle for xenogeneic corneal scaffolds, but does not establish the claim across many types of dECM scaffold or directly assess their clinical translation. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • The application of dECM bioscaffolds still confronts major immunologic challenges.

    “However, the application of these bioscaffolds still confronts major immunologic challenges.”

    From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [33]

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Cited by

Sorted by citations

Authors
Sharifi, R., Yang, Y., Adibnia, Y., Dohlman, C. H., Chodosh, J., & Gonzalez-Andrades, M.
Affiliations
  • Massachusetts Eye and Ear Infirmary (US)
  • Yeditepe University (TR)
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2019-02-12
Volume
9
Issue
1
Pages
1876-1876
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-018-38342-4
PMC
PMC6372616
OpenAlex
W2921956590
Cited by
51
References
4
Citations by year
2026: 8 · 2025: 5 · 2024: 9 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 8 · 2019: 2
FWCI
5.26
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RISalud Institutional Health Repository of Andalucía (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
porcine cornea, species selection, amino acid sequence similarity, decorin, lumican, keratocan, isoelectric point, GRAVY, proline content, xenotransplantation, corneal transplantation
MeSH terms
Lumican, Algorithms, Animals, Collagen, Extracellular Matrix, Eye Proteins, Horses, Humans, Isoelectric Point, Neoplasm Transplantation, Phylogeny, Proline, Proteoglycans, Species Specificity, Swine, Transplantation, Heterologous, Corneal Transplantation, Sequence Alignment, Computational Biology, Decorin, Heterografts

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