Cited by 314Telomeres, Telomerase, and SenescenceDNA and Nucleic Acid ChemistryDNA Repair Mechanisms

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

POT1 protects telomeres from a transient DNA damage response and determines how human chromosomes end

Open access

Hockemeyer, Dirk, Sfeir, Agnel J, Shay, Jerry W, Wright, Woodring E, de Lange, Titia·2005·The EMBO Journal

DOI 10.1038/sj.emboj.7600733PMC PMC1176460

Open this paper in CoChat — free account Open PDF

Abstract

The hallmarks of telomere dysfunction in mammals are reduced telomeric 3' overhangs, telomere fusions, and cell cycle arrest due to a DNA damage response. Here, we report on the phenotypes of RNAi-mediated inhibition of POT1, the single-stranded telomeric DNA-binding protein. A 10-fold reduction in POT1 protein in tumor cells induced neither telomere fusions nor cell cycle arrest. However, the 3' overhang DNA was reduced and all telomeres elicited a transient DNA damage response in G1, indicating that extensive telomere damage can occur without cell cycle arrest or telomere fusions. RNAi to POT1 also revealed its role in generating the correct sequence at chromosome ends. The recessed 5' end of the telomere, which normally ends on the sequence ATC-5', was changed to a random position within the AATCCC repeat. Thus, POT1 determines the structure of the 3' and 5' ends of human chromosomes, and its inhibition generates a novel combination of telomere dysfunction phenotypes in which chromosome ends behave transiently as sites of DNA damage, yet remain protected from nonhomologous end-joining.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/sj.emboj.7600733

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.20.9363 · Cited by 7,477
A DNA damage checkpoint response in telomere-initiated senescence
Fagagna, F. D. D., Reaper, P. M., Clay-Farrace, L., Fiegler, H., Carr, P., von Zglinicki, T., Saretzki, G., Carter, N. P., & Jackson, S. P. (2003) · Nature · doi:10.1038/nature02118 · Cited by 2,917 · cited 1×
“In addition, dysfunctional telomeres can induce the ATM kinase and become associated with DNA damage response factors such as 53BP1, g-H2AX, and the Mre11 complex (d’Adda di Fagagna et al, 2003; Takai et al, 2003; Herbig et al, 2004; Celli and de Lange, 2005).”
Background
Mammalian Telomeres End in a Large Duplex Loop
Griffith, J. D., Comeau, L., Rosenfield, S., Stansel, R. M., Bianchi, A., Moss, H., & de Lange, T. (1999) · Cell · doi:10.1016/s0092-8674(00)80760-6 · Cited by 2,447
Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.
Counter, C. M., Avilion, A. A., LeFeuvre, C. E., Stewart, N. G., Greider, C. W., Harley, C. B., & Bacchetti, S. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05245.x · Cited by 2,096 · cited 1×
“NHEJ-mediated chromosome end fusions are a prominent consequence of TRF2 inhibition and can occur when telomeres become critically short (Counter et al, 1992; van Steensel et al, 1998; Smogorzewska et al, 2002).”
Background
TRF2 Protects Human Telomeres from End-to-End Fusions
van Steensel, B., Smogorzewska, A., & de Lange, T. (1998) · Cell · doi:10.1016/s0092-8674(00)80932-0 · Cited by 1,709
DNA Damage Foci at Dysfunctional Telomeres
Takai, H., Smogorzewska, A., & de Lange, T. (2003) · Current Biology · doi:10.1016/s0960-9822(03)00542-6 · Cited by 1,409
Telomere Shortening Triggers Senescence of Human Cells through a Pathway Involving ATM, p53, and p21CIP1, but Not p16INK4a
Herbig, U., Jobling, W. A., Chen, B. P. C., Chen, D. J., & Sedivy, J. M. (2004) · Molecular Cell · doi:10.1016/s1097-2765(04)00256-4 · Cited by 1,344
p53- and ATM-Dependent Apoptosis Induced by Telomeres Lacking TRF2
Karlseder, J., Broccoli, D., Dai, Y., Hardy, S., & de Lange, T. (1999) · Science · doi:10.1126/science.283.5406.1321 · Cited by 1,086
Pot1, the Putative Telomere End-Binding Protein in Fission Yeast and Humans
Baumann, P., & Cech, T. R. (2001) · Science · doi:10.1126/science.1060036 · Cited by 1,028
Long G Tails at Both Ends of Human Chromosomes Suggest a C Strand Degradation Mechanism for Telomere Shortening
Makarov, V. L., Hirose, Y., & Langmore, J. P. (1997) · Cell · doi:10.1016/s0092-8674(00)81908-x · Cited by 959

Show all 34 references

Sorted by citations

Shelterin: the protein complex that shapes and safeguards human telomeres
de Lange, T. (2005) · Genes & Development · doi:10.1101/gad.1346005 · Cited by 3,056
How Shelterin Protects Mammalian Telomeres
Palm, W., & de Lange, T. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.41.110306.130350 · Cited by 1,802
Protection of telomeres through independent control of ATM and ATR by TRF2 and POT1
Denchi, E. L., & de Lange, T. (2007) · Nature · doi:10.1038/nature06065 · Cited by 880
G-quadruplex structures: in vivo evidence and function
Lipps, H. J., & Rhodes, D. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2009.05.002 · Cited by 856
Telomeres and telomerase in cancer
Artandi, S. E., & DePinho, R. A. (2010) · Carcinogenesis · doi:10.1093/carcin/bgp268 · Cited by 823
Targeting telomeres and telomerase
Cian, A. D., Lacroix, L., Douarre, C., Temime‐Smaali, N., Trentesaux, C., Riou, J., & Mergny, J. (2007) · Biochimie · doi:10.1016/j.biochi.2007.07.011 · Cited by 583
Telomere length assessment: Biomarker of chronic oxidative stress?
Houben, J. M. J., Moonen, H. J. J., van Schooten, F., & Hageman, G. J. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.10.001 · Cited by 577
Markers of cellular senescence. Telomere shortening as a marker of cellular senescence
Bernadotte, A., Mikhelson, V. M., & Spivak, I. M. (2016) · Aging · doi:10.18632/aging.100871 · Cited by 466
Replication and protection of telomeres
Verdun, R. E., & Karlseder, J. (2007) · Nature · doi:10.1038/nature05976 · Cited by 463
How telomeres are replicated
Gilson, É., & Géli, V. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2259 · Cited by 496
Pot1 Deficiency Initiates DNA Damage Checkpoint Activation and Aberrant Homologous Recombination at Telomeres
Wu, L., Multani, A. S., He, H., Cosme-Blanco, W., Deng, Y., Deng, J. M., Bachilo, O., Pathak, S., Tahara, H., Bailey, S. M., et al. (2006) · Cell · doi:10.1016/j.cell.2006.05.037 · Cited by 422
ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cellsPreprint
Wong, L. H., McGhie, J. D. R., Sim, M., Anderson, M. A., Ahn, S., Hannan, R. D., George, A. J., Morgan, K. A., Mann, J. R., & Choo, K. H. A. (2010) · Genome Research · doi:10.1101/gr.101477.109 · Cited by 435
Recent Expansion of the Telomeric Complex in Rodents: Two Distinct POT1 Proteins Protect Mouse Telomeres
Hockemeyer, D., Daniels, J.-P., Takai, H., & de Lange, T. (2006) · Cell · doi:10.1016/j.cell.2006.04.044 · Cited by 397
Self-citation
RPA-like Mammalian Ctc1-Stn1-Ten1 Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Pot1 Pathway
Miyake, Y., Nakamura, M., Nabetani, A., Shimamura, S., Tamura, M., Yonehara, S., Saito, M., & Ishikawa, F. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.08.009 · Cited by 350
Genomic instability in human cancer: Molecular insights and opportunities for therapeutic attack and prevention through diet and nutrition
Ferguson, L. R., Chen, H., Collins, A. R., Connell, M., Damia, G. L. M., Dasgupta, S., Malhotra, M., Meeker, A. K., Amedei, A., Amin, A., et al. (2015) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2015.03.005 · Cited by 352
Telomeric 3′ Overhangs Derive from Resection by Exo1 and Apollo and Fill-In by POT1b-Associated CST
Wu, P. V., Takai, H., & de Lange, T. (2012) · Cell · doi:10.1016/j.cell.2012.05.026 · Cited by 348
Conserved Telomere Maintenance Component 1 Interacts with STN1 and Maintains Chromosome Ends in Higher Eukaryotes
Surovtseva, Y. V., Churikov, D., Boltz, K. A., Song, X., Lamb, J. C., Warrington, R. T., Leehy, K. A., Heacock, M. L., Price, C. M., & Shippen, D. E. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.09.017 · Cited by 304
Cellular Senescence in Neurodegenerative Diseases
Martínez‐Cué, C., & Rueda, N. (2020) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2020.00016 · Cited by 299
POT1 mutations cause telomere dysfunction in chronic lymphocytic leukemia
Ramsay, A. J., Quesada, V., Foronda, M., Conde, L., Martínez‐Trillos, A., Villamor, N., Rodrı́guez, D., Kwarciak, A., Garabaya, C., Gallardo, M., et al. (2013) · Nature Genetics · doi:10.1038/ng.2584 · Cited by 274
Mutations in the promoter of the telomerase gene TERT contribute to tumorigenesis by a two-step mechanism
Chiba, K., Lorbeer, F. K., Shain, A. H., McSwiggen, D. T., Schruf, E., Oh, A., Ryu, J., Darzacq, X., Bastian, B. C., & Hockemeyer, D. F. (2017) · Science · doi:10.1126/science.aao0535 · Cited by 283
Self-citation
Shaping human telomeres: from shelterin and CST complexes to telomeric chromatin organization
Lim, C. J., & Cech, T. R. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00328-y · Cited by 273
Telomere damage induced by the G-quadruplex ligand RHPS4 has an antitumor effect
Salvati, E., Leonetti, C., Rizzo, A., Scarsella, M., Mottolese, M., Galati, R., Sperduti, I., Stevens, M. F. G., D’Incalci, M., Blasco, M., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci32461 · Cited by 266
Cancer-associated TERT promoter mutations abrogate telomerase silencing
Chiba, K., Johnson, J. Z., Vogan, J. M., Wagner, T., Boyle, J. M., & Hockemeyer, D. F. (2015) · eLife · doi:10.7554/elife.07918 · Cited by 257
Self-citation
In Vivo Stoichiometry of Shelterin Components
Takai, K., Hooper, S., Blackwood, S., Gandhi, R., & de Lange, T. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.038026 · Cited by 258
TIN2-Tethered TPP1 Recruits Human Telomerase to Telomeres In Vivo
Abreu, E. B., Aritonovska, E., Reichenbach, P., Cristofari, G., Culp, B. R., Terns, R. M., Lingner, J., & Terns, M. P. (2010) · Molecular and Cellular Biology · doi:10.1128/mcb.00240-10 · Cited by 258
Germline Mutations in Shelterin Complex Genes Are Associated With Familial Glioma
Bainbridge, M. N., Armstrong, G., Gramatges, M. M., Bertuch, A. A., Jhangiani, S. N., Doddapaneni, H., Lewis, L. R., Tombrello, J., Tsavachidis, S., Liu, Y., et al. (2014) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/dju384 · Cited by 227
Five dysfunctional telomeres predict onset of senescence in human cells
Kaul, Z., Cesare, A. J., Huschtscha, L. I., Neumann, A. A., & Reddel, R. R. (2012) · The EMBO Reports · doi:10.1038/embor.2011.227 · Cited by 225
“Telomere length-independent deprotection of telomeres via disruption of shelterin proteins induces p53-dependent senescence (van Steensel et al , 1998; Hockemeyer et al , 2005; Guo et al , 2007).”
A RAP1/TRF2 Complex Inhibits Nonhomologous End-Joining at Human Telomeric DNA Ends
Bae, N. S., & Baumann, P. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.03.023 · Cited by 215
POT1 Stimulates RecQ Helicases WRN and BLM to Unwind Telomeric DNA Substrates
Opresko, P. L., Mason, P. A., Podell, E. R., Lei, M., Hickson, I. D., Cech, T. R., & Bohr, V. A. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m505211200 · Cited by 211
Apollo, an Artemis-Related Nuclease, Interacts with TRF2 and Protects Human Telomeres in S Phase
van Overbeek, M., & de Lange, T. (2006) · Current Biology · doi:10.1016/j.cub.2006.05.022 · Cited by 204
Telomeric Strategies: Means to an End
Jain, D., & Cooper, J. P. (2010) · Annual Review of Genetics · doi:10.1146/annurev-genet-102108-134841 · Cited by 205
Characterization of Oxidative Guanine Damage and Repair in Mammalian Telomeres
Wang, Z., Rhee, D. B., Lü, J., Bohr, C. T., Fang, Z., Vallabhaneni, H., de Souza-Pinto, N. C., & Liu, Y. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000951 · Cited by 199
Telomere protection by mammalian Pot1 requires interaction with Tpp1
Hockemeyer, D. F., Palm, W., Else, T., Daniels, J., Takai, K., Ye, J. Z.-S., Keegan, C. E., de Lange, T., & Hammer, G. D. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1270 · Cited by 214
Self-citation
POT1–TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation
Latrick, C. M., & Cech, T. R. (2010) · The EMBO Journal · doi:10.1038/emboj.2009.409 · Cited by 195
Histone H3.3 incorporation provides a unique and functionally essential telomeric chromatin in embryonic stem cellsPreprint
Wong, L. H., Ren, H., Williams, E., McGhie, J. B., Ahn, S.-Y., Sim, M., Tam, A., Earle, E., Anderson, M. A., Mann, J. R., et al. (2009) · Genome Research · doi:10.1101/gr.084947.108 · Cited by 181
Oligonucleotide/oligosaccharide-binding fold proteins: a growing family of genome guardians
Flynn, R. L., & Zou, L. (2010) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2010.488216 · Cited by 186
DNA damage response at functional and dysfunctional telomeresPreprint
Longhese, M. P. (2008) · Genes & Development · doi:10.1101/gad.1626908 · Cited by 184
Oxidative stress, DNA damage, and the telomeric complex as therapeutic targets in acute neurodegeneration
Smith, J. A., Park, S., Krause, J. S., & Banik, N. L. (2013) · Neurochemistry International · doi:10.1016/j.neuint.2013.02.013 · Cited by 173
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells
Conomos, D., Stutz, M. D., Hills, M., Neumann, A. A., Bryan, T. M., Reddel, R. R., & Pickett, H. A. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.201207189 · Cited by 177
Checkpoint-dependent phosphorylation of Exo1 modulates the DNA damage response
Morin, I., Ngo, H., Greenall, A., Zubko, M. K., Morrice, N., & Lydall, D. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.171 · Cited by 164
A method for measuring the distribution of the shortest telomeres in cells and tissues
Lai, T., Zhang, N., Noh, J., Mender, I., Tedone, E., Huang, E. E., Wright, W. E., Danuser, G. M., & Shay, J. W. (2017) · Nature Communications · doi:10.1038/s41467-017-01291-z · Cited by 174
Self-citation
Mechanisms of telomere loss and their consequences for chromosome instability
Muraki, K., Nyhan, K. C., Han, L., & Murnane, J. P. (2012) · Frontiers in Oncology · doi:10.3389/fonc.2012.00135 · Cited by 173
Telomeres, interstitial telomeric repeat sequences, and chromosomal aberrations
Bolzán, A. D., & de Bianchi, M. S. A. (2006) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2005.12.003 · Cited by 151
The Role of Double-Strand Break Repair Pathways at Functional and Dysfunctional TelomeresPreprint
Doksani, Y., & de Lange, T. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016576 · Cited by 143
NuRD–ZNF827 recruitment to telomeres creates a molecular scaffold for homologous recombination
Conomos, D., Reddel, R. R., & Pickett, H. A. (2014) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2877 · Cited by 129
POT1-TPP1 Regulates Telomeric Overhang Structural Dynamics
Hwang, H. H., Buncher, N. A., Opresko, P. L., & Myong, S. (2012) · Structure · doi:10.1016/j.str.2012.08.018 · Cited by 136
Functional interaction between telomere protein TPP1 and telomerasePreprint
Zaug, A. J., Podell, E. R., Nandakumar, J., & Cech, T. R. (2010) · Genes & Development · doi:10.1101/gad.1881810 · Cited by 139
DNA Degradation at Unprotected Telomeres in Yeast Is Regulated by the CDK1 (Cdc28/Clb) Cell-Cycle Kinase
Vodenicharov, M. D., & Wellinger, R. J. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.07.035 · Cited by 121
Functional Dissection of Human and Mouse POT1 Proteins
Palm, W., Hockemeyer, D. F., Kibe, T., & de Lange, T. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.01352-08 · Cited by 130
Self-citation
The Role of the Nonhomologous End-Joining DNA Double-Strand Break Repair Pathway in Telomere Biology
Říha, K., Heacock, M. L., & Shippen, D. E. (2006) · Annual Review of Genetics · doi:10.1146/annurev.genet.39.110304.095755 · Cited by 122
Telomere uncapping and alternative lengthening of telomeres
Cesare, A. J., & Reddel, R. R. (2007) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2007.11.006 · Cited by 125
Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis
Pinzaru, A. M., Hom, R. A., Beal, A., Phillips, A. F., Ni, E., Cardozo, T. J., Nair, N., Choi, J., Wuttke, D. S., Sfeir, A. J., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.05.008 · Cited by 114
Self-citation
Quantitative telomeric overhang determination using a double-strand specific nuclease
Zhao, Y. B., Hoshiyama, H., Shay, J. W., & Wright, W. E. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm1063 · Cited by 115
Self-citation
Structural and functional analysis of the human POT1-TPP1 telomeric complex
Rice, C., Shastrula, P. K., Kossenkov, A. V., Hills, R., Baird, D. M., Showe, L. C., Doukov, T. I., Janicki, S. M., & Skordalakes, E. (2017) · Nature Communications · doi:10.1038/ncomms14928 · Cited by 112
Telomere Maintenance through Spatial Control of Telomeric Proteins
Chen, L., Liu, D., & Songyang, Z. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00603-07 · Cited by 117
Genetic and molecular identification of three human TPP1 functions in telomerase action: recruitment, activation, and homeostasis set point regulationPreprint
Sexton, A. N., Regalado, S. G., Lai, C. S., Cost, G. J., O’Neil, C. M., Urnov, F. D., Gregory, P. D., Jaenisch, R., Collins, K., & Hockemeyer, D. F. (2014) · Genes & Development · doi:10.1101/gad.246819.114 · Cited by 112
Self-citation
G-quadruplexes: targets and tools in anticancer drug design
Düchler, M. (2012) · Journal of drug targeting · doi:10.3109/1061186x.2012.669384 · Cited by 109
A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plusPreprint
Takai, H., Jenkinson, E. M., Kabir, S., Babul‐Hirji, R., Najm-Tehrani, N., Chitayat, D. A., Crow, Y. J., & de Lange, T. (2016) · Genes & Development · doi:10.1101/gad.276873.115 · Cited by 109
Single Molecule Studies of Physiologically Relevant Telomeric Tails Reveal POT1 Mechanism for Promoting G-quadruplex Unfolding
Wang, H., Nora, G. J., Ghodke, H. B., & Opresko, P. L. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.205641 · Cited by 104
Human Stn1 protects telomere integrity by promoting efficient lagging-strand synthesis at telomeres and mediating C-strand fill-in
Huang, C., Dai, X., & Chai, W. (2012) · Cell Research · doi:10.1038/cr.2012.132 · Cited by 106
The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G‐overhangs at human telomeres
Chai, W., Sfeir, A. J., Hoshiyama, H., Shay, J. W., & Wright, W. E. (2005) · EMBO Reports · doi:10.1038/sj.embor.7400600 · Cited by 102
Self-citation
Separation of telomerase functions by reverse genetics
Mukherjee, S., Firpo, E. J., Wang, Y., & Roberts, J. M. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1112414108 · Cited by 104
Telomere targeting with a novel G-quadruplex-interactive ligand BRACO-19 induces T-loop disassembly and telomerase displacement in human glioblastoma cells
Zhou, G., Liu, X., Li, Y., Xu, S., Ma, C., Wu, X., Cheng, Y., Yu, Z., Zhao, G., & Chen, Y. (2016) · Oncotarget · doi:10.18632/oncotarget.7483 · Cited by 107
SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair
Hou, T., Cao, Z., Zhang, J., Tang, M., Tian, Y., Li, Y., Lu, X., Chen, Y., Wang, H., Wei, F., et al. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa006 · Cited by 96
Spreading of mammalian DNA‐damage response factors studied by ChIP‐chip at damaged telomeres
Meier, A., Fiegler, H., Muñoz, P., Ellis, P., Rigler, D., Langford, C., Blasco, M. A., Carter, N., & Jackson, S. P. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601719 · Cited by 100
Arabidopsis POT1 associates with the telomerase RNP and is required for telomere maintenance
Surovtseva, Y. V., Shakirov, E. V., Vespa, L., Osbun, N. C., Song, X., & Shippen, D. E. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601792 · Cited by 97
Telomere capping in non‐dividing yeast cells requires Yku and Rap1
Vodenicharov, M. D., Laterreur, N., & Wellinger, R. J. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.155 · Cited by 87
The Response to DNA Damage at Telomeric Repeats and Its Consequences for Telomere Function
Doksani, Y. (2019) · Genes · doi:10.3390/genes10040318 · Cited by 83
RecQ helicases in DNA double strand break repair and telomere maintenance
Singh, D. K., Ghosh, A. K., Croteau, D. L., & Bohr, V. A. (2011) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2011.06.002 · Cited by 76
PML bodies provide an important platform for the maintenance of telomeric chromatin integrity in embryonic stem cells
Chang, F. T. M., McGhie, J. D. R., Chan, F. L., Tang, M. C. W., Anderson, M. A., Mann, J. R., Choo, K. H. A., & Wong, L. H. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt114 · Cited by 83
Human Telomeres and Telomere Biology Disorders
Savage, S. A. (2014) · Progress in molecular biology and translational science · doi:10.1016/b978-0-12-397898-1.00002-5 · Cited by 78
Role of POT1 in Human Cancer
Wu, Y., Poulos, R. C., & Reddel, R. R. (2020) · Cancers · doi:10.3390/cancers12102739 · Cited by 73
Pot1 and telomere maintenance
Baumann, P., & Price, C. M. (2010) · FEBS Letters · doi:10.1016/j.febslet.2010.05.024 · Cited by 78
Vertebrate POT1 Restricts G-Overhang Length and Prevents Activation of a Telomeric DNA Damage Checkpoint but Is Dispensable for Overhang Protection
Churikov, D., Wei, C., & Price, C. M. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01011-06 · Cited by 75
Telomeric Overhang Length Determines Structural Dynamics and Accessibility to Telomerase and ALT-Associated Proteins
Hwang, H. H., Kreig, A., Calvert, J. S., Lormand, J. D., Kwon, Y., Daley, J. M., Sung, P., Opresko, P. L., & Myong, S. (2014) · Structure · doi:10.1016/j.str.2014.03.013 · Cited by 71
Pot1 and cell cycle progression cooperate in telomere length regulation
Churikov, D., & Price, C. M. (2008) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1331 · Cited by 67
Control of Cellular Aging, Tissue Function, and Cancer by p53 Downstream of TelomeresPreprint
Roake, C. M., & Artandi, S. E. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a026088 · Cited by 70
Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear porePreprint
Pinzaru, A. M., Kareh, M., Lamm, N., Denchi, E. L., Cesare, A. J., & Sfeir, A. J. (2020) · Genes & Development · doi:10.1101/gad.337287.120 · Cited by 68
Self-citation
Telomere structure and function in trypanosomes: a proposal
Dreesen, O., Li, B., & Cross, G. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1577 · Cited by 65
Human POT1 is required for efficient telomere C-rich strand replication in the absence of WRNPreprint
Arnoult, N., Saintomé, C., Ourliac‐Garnier, I., Riou, J., & Londoño‐Vallejo, A. (2009) · Genes & Development · doi:10.1101/gad.544009 · Cited by 70
Molecular steps of G‐overhang generation at human telomeres and its function in chromosome end protection
Dai, X., Huang, C., Bhusari, A., Sampathi, S., Schubert, K. M., & Chai, W. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.156 · Cited by 73
Crosstalk between telomere maintenance and radiation effects: A key player in the process of radiation-induced carcinogenesis
Shim, G., Ricoul, M., Hempel, W. M., Azzam, E. I., & Sabatier, L. (2014) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2014.01.001 · Cited by 66
The Protein Network Surrounding the Human Telomere Repeat Binding Factors TRF1, TRF2, and POT1
Giannone, R. J., McDonald, W. H., Hurst, G. B., Shen, R., Wang, Y., & Liu, Y. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012407 · Cited by 64
Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein–Barr virus
Kamranvar, S. A., Chen, X., & Masucci, M. G. (2013) · Oncogene · doi:10.1038/onc.2013.189 · Cited by 61
Telomere dysfunction and genome instability
Frías-López, C. (2012) · Frontiers in bioscience · doi:10.2741/4044 · Cited by 66
Clonality of the stem cell compartment during evolution of myelodysplastic syndromes and other bone marrow failure syndromes
Tiu, R. V., Gondek, L. P., O'Keefe, C., & Maciejewski, J. P. (2007) · Leukemia · doi:10.1038/sj.leu.2404757 · Cited by 60
Analysis of muntjac deer genome and chromatin architecture reveals rapid karyotype evolution
Mudd, A. B., Bredeson, J. V., Baum, R., Hockemeyer, D. F., & Rokhsar, D. S. (2020) · Communications Biology · doi:10.1038/s42003-020-1096-9 · Cited by 61
Self-citation
Telomere dysfunction and cell survival: roles for distinct TIN2-containing complexes
Kim, S., Davalos, A. R., Heo, S., Rodier, F., Zou, Y. S., Beausejour, C. M., Kaminker, P. G., Yannone, S. M., & Campisi, J. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200710028 · Cited by 62
Telomeric armor: the layers of end protection
Oganesian, L., & Karlseder, J. (2009) · Journal of Cell Science · doi:10.1242/jcs.050567 · Cited by 61
Give me a break: How telomeres suppress the DNA damage response
Denchi, E. L. (2009) · DNA repair · doi:10.1016/j.dnarep.2009.04.013 · Cited by 61
G-Quadruplex binding enantiomers show chiral selective interactions with human telomere
Wang, J., Chen, Y., Ren, J., Zhao, C., & Qu, X. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1354 · Cited by 56
Taming the tiger by the tail: modulation of DNA damage responses by telomeres
Lydall, D. A. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.176 · Cited by 57
Structural identity of telomeric complexes
Giraud-Panis, M.-J. E., Pisano, S., Poulet, A., Du, M. L., & Gilson, É. (2010) · FEBS Letters · doi:10.1016/j.febslet.2010.08.004 · Cited by 67
The MRT‐1 nuclease is required for DNA crosslink repair and telomerase activity in vivo in Caenorhabditis elegans
Meier, B., Barber, L. J., Liu, Y., Shtessel, L., Boulton, S. J., Gartner, A., & Ahmed, S. C. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.278 · Cited by 54
The human telomere and its relationship to human disease, therapy, and tissue engineering
Ian, K. M. (2007) · Frontiers in bioscience · doi:10.2741/2412 · Cited by 55
Cancer‐associated POT1 mutations lead to telomere elongation without induction of a DNA damage response
Kim, W. T., Hennick, K. M., Johnson, J. Z., Finnerty, B., Choo, S.-A. S., Short, S. B., Drubin, C. W., Forster, R. E., McMaster, M. L., & Hockemeyer, D. F. (2021) · The EMBO Journal · doi:10.15252/embj.2020107346 · Cited by 55
Self-citation
The maintenance and masking of chromosome termini
Bertuch, A. A., & Lundblad, V. (2006) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2006.04.005 · Cited by 52
Human shelterin protein POT1 prevents severe telomere instability induced by homology‐directed DNA repair
Glousker, G., Briod, A., Quadroni, M., & Lingner, J. (2020) · The EMBO Journal · doi:10.15252/embj.2020104500 · Cited by 51
Cdc13 Telomere Capping Decreases Mec1 Association but Does Not Affect Tel1 Association with DNA Ends
Hirano, Y., & Sugimoto, K. (2007) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-12-1074 · Cited by 51
POT1 mutation spectrum in tumour types commonly diagnosed among POT1-associated hereditary cancer syndrome families
Shen, E., Xiu, J., Lopez, G. Y., Bentley, R. C., Jalali, A., Heimberger, A. B., Bainbridge, M. N., Bondy, M. L., & Walsh, K. M. (2020) · Journal of Medical Genetics · doi:10.1136/jmedgenet-2019-106657 · Cited by 49

Authors: Hockemeyer, D., Sfeir, A. J., Shay, J. W., Wright, W. E., & de Lange, T.
Affiliations: Rockefeller University (US)
The University of Texas Southwestern Medical Center (US)
ORCID: Hockemeyer, Dirk
Sfeir, Agnel J
Shay, Jerry W
Wright, Woodring E
Venue: The EMBO Journal
Publisher: Springer Science and Business Media LLC
Published: 2005-06-23
Volume: 24
Issue: 14
Pages: 2667-2678
ISSN: 0261-4189 (ISSN-L)
1460-2075
DOI: 10.1038/sj.emboj.7600733
PMC: PMC1176460
OpenAlex: W2138398263
Cited by: 314
References: 34
Citations by year: 2026: 9 · 2025: 8 · 2024: 9 · 2023: 11 · 2022: 3 · 2021: 7 · 2020: 16 · 2019: 11 · 2018: 6 · 2017: 15 · 2016: 14 · 2015: 13 · 2014: 21 · 2013: 13 · 2012: 21
FWCI: 12.84
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: The EMBO Journal (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: U.S. Department of Defense
Ellison Medical Foundation
National Institutes of Health ((AG01228), GM49046)
National Institute on Aging (R56AG016642, AG 01228, AG16642, R01AG001228, R01AG016642)
National Institute of General Medical Sciences (GM 49046, R37GM049046, R01GM049046)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords: POT1, telomere dysfunction, DNA damage response, chromosome ends, telomere fusion, cell cycle arrest, non-homologous end joining, telomeric repeats, RNAi knockdown, cancer cells
MeSH terms: Shelterin Complex, Chromosomes, Human, DNA Damage, HeLa Cells, Humans, Telomere, Alternative Splicing, Protein Isoforms, Telomere-Binding Proteins, Cell Proliferation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

POT1 protects telomeres from a transient DNA damage response and determines how human chromosomes end

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks