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Cited by 585Adipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismCardiovascular Disease and Adiposity

The DOI matches its Crossref record; 62 of the 64 references it cites were found in a registry or an index.

Obesity in C57BL/6J mice is characterized by adipose tissue hypoxia and cytotoxic T-cell infiltration

Rausch, M E, Weisberg, S, Vardhana, P, Tortoriello, D V·2008·International Journal of Obesity
DOI 10.1038/sj.ijo.0803744
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Abstract

Background Obesity is currently viewed as a state of chronic low-grade inflammation in which there is a pro-inflammatory alteration in the serum adipocytokine profile as well as an infiltration of white adipose tissue by activated macrophages. The etiology of this inflammation, however, is poorly understood.Methods Hypothesizing that local hypoxia within expanding white adipose tissue depots may contribute to obesity-related inflammation, we compared body composition, serum inflammatory marker concentrations and the expression of several hypoxia-regulated genes in white adipose tissue derived from lean, dietary-induced obese (DIO) and ob/ob male C57BL/6J mice. We also examined white adipose tissue for the presence of hypoxia using both a pimonidazole-based antibody system and a fiberoptic sensor for real-time pO(2) quantification in vivo. Finally, using cell-specific leukocyte antibodies, we performed immunohistochemistry and flow cytometric analyses to further characterize the cellular nature of adipose inflammation.Results We determined that obesity in male C57BL/6J mice is associated with increased expression of HIF (hypoxia-inducible factor) isoforms and GLUT-1, and that white adipose tissue hypoxia was present in the obese mice. Immunohistochemistry revealed hypoxic areas to colocalize predominantly with F4/80+ macrophages. Interestingly, CD3+ T cells were present in large numbers within the adipose of both DIO and ob/ob obese mice, and flow cytometry revealed their adipose to possess significantly more CD8+ T cells than their lean cohort.Conclusions White adipose hypoxia and cytotoxic T-cell invasion are features of obesity in C57BL/6J mice and are potential contributors to their local and generalized inflammatory state.

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Metadata

Authors
Rausch, M. E., Weisberg, S., Vardhana, P., & Tortoriello, D. V.
Affiliations
Columbia University (US)
Corresponding author
Tortoriello, D V
ORCID
Venue
International Journal of Obesity
Publisher
Springer Science and Business Media LLC
Published
2007-09-25
Volume
32
Issue
3
Pages
451-463
ISSN
  • 0307-0565 (ISSN-L)
  • 1476-5497
DOI
10.1038/sj.ijo.0803744
OpenAlex
W2160915024
Cited by
585
References
64
Citations by year
2026: 7 · 2025: 17 · 2024: 18 · 2023: 19 · 2022: 23 · 2021: 29 · 2020: 28 · 2019: 29 · 2018: 33 · 2017: 50 · 2016: 31 · 2015: 48 · 2014: 42 · 2013: 45 · 2012: 39
FWCI
17.20
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • International Journal of Obesity (publishedVersion)
  • PubMed (publishedVersion)
Funders
Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD001275)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
Keywords
obesity, adipose tissue inflammation, cytotoxic T lymphocytes, CD8+ T cells, hypoxia-inducible factor, GLUT1, macrophages, F4/80+ macrophages, pimonidazole, ob/ob mice, diet-induced obesity, C57BL/6J mice
MeSH terms
Adipose Tissue, Animals, Immunohistochemistry, Macrophages, Male, Mice, Inbred C57BL, Mice, Obese, Obesity, T-Lymphocytes, Cytotoxic, Cell Hypoxia, CD3 Complex, CD8-Positive T-Lymphocytes, Glucose Transporter Type 1, Mice, Hypoxia-Inducible Factor 1

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