Time Course of Transgene Expression After Intrastriatal Pseudotyped rAAV2/1, rAAV2/2, rAAV2/5, and rAAV2/8 Transduction in the Rat
Open accessAbstract
In vivo recombinant adeno-associated viral vector (rAAV)-mediated transduction of various tissues including brain has been characterized by slow onset and gradual increase in gene expression before reaching stable long-term protein levels. The early time course of transgene expression has not been quantified using newly available rAAV capsid serotypes. In this experiment, the onset of expression of green fluorescent protein (GFP) after intrastriatal injection of rAAV2-based pseudotyped vectors (rAAV1, rAAV5, and rAAV8 capsids) was quantified. Native GFP fluorescence displayed a delayed onset of expression of at least 7 days for all the pseudotyped rAAV vectors. However, GFP immunohistochemical staining revealed significant transgene expression by 4 days after transduction for all serotypes and stable GFP(+) neuronal populations mediated by all serotypes within 14 days post transduction at the latest. rAAV2/1 and rAAV2/2 displayed no time-dependent increase of GFP(+) striatal neurons; reaching maximal striatal cell GFP(+) counts at 4 days after injection. All serotypes displayed peak transgene expression by 4 weeks post injection where native GFP(+) neurons were equal to immunostained striatal GFP(+) neurons. The inflammatory response to these rAAV vectors was present up to 4 weeks after transduction but was not apparent 9 months post injection. Thus, rAAV-mediated transgene expression begins earlier than previously thought.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In previous work, a capsid and/or transgene related inflammatory response peaks at 2-weeks post-injection and then retracts to the needle track.
“In our previous work, we have seen that a capsid and/or transgene related inflammatory response peaks at 2-weeks post-injection and then retracts to the needle track [46], similarly to what is seen with the Olig001-GFP injected monkeys.”
From Novel oligodendroglial alpha synuclein viral vector models of multiple system atrophy: studies in rodents and nonhuman primates (2017) · cites this paper as [46]
Cites
Efficient selection for high-expression transfectants with a novel eukaryotic vector
Niwa, H., Yamamura, K., & Miyazaki, J. (1991) · Gene · doi:10.1016/0378-1119(91)90434-d · Cited by 5,135 · see citing works
West, M. J., Slomianka, L., & Gundersen, H. J. G. (1991) · The Anatomical Record · doi:10.1002/ar.1092310411 · Cited by 2,994 · see citing works
Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy
Gao, G.-P., Alvira, M. R., Wang, L., Calcedo, R., Johnston, J., & Wilson, J. M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.182412299 · Cited by 1,520 · see citing works
The efficiency of systematic sampling in stereology—reconsidered
GUNDERSEN, H. J. G., JENSEN, E. B. V., KIÊU, K., & NIELSEN, J. (1999) · Journal of Microscopy · doi:10.1046/j.1365-2818.1999.00457.x · Cited by 1,317 · see citing works
Ferrari, F. K., Samulski, T., Shenk, T., & Samulski, R. J. (1996) · Journal of Virology · doi:10.1128/jvi.70.5.3227-3234.1996 · Cited by 761 · see citing works
Burger, C., Gorbatyuk, O. S., Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. J., & Muzyczka, N. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.05.024 · Cited by 741 · see citing works
Self-citationDavidson, B. L., Stein, C. S., Heth, J., Martins, I., Kotin, R. M., Derksen, T. A., Zabner, J., Ghodsi, A., & Chiorini, J. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.050581197 · Cited by 679 · see citing works
Kessler, P., Podsakoff, G. M., Chen, X., McQuiston, S. A., Colosi, P., Matelis, L. A., Kurtzman, G. J., & Byrne, B. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.14082 · Cited by 614 · see citing works
A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells
Zolotukhin, S., Potter, M., Hauswirth, W. W., Guy, J., & Muzyczka, N. (1996) · Journal of Virology · doi:10.1128/jvi.70.7.4646-4654.1996 · Cited by 609 · see citing works
Self-citationProduction and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors
Zolotukhin, S., Potter, M., Zolotukhin, I., Sakai, Y., Loiler, S., Fraites, T. J., Chiodo, V. A., Philipsberg, T., Muzyczka, N., Hauswirth, W. W., et al. (2002) · Methods · doi:10.1016/s1046-2023(02)00220-7 · Cited by 556 · see citing works
Self-citationHerzog, R. W., Yang, E. Y., Couto, L. B., Hagstrom, J. N., Elwell, D., Fields, P. A., Burton, M., Bellinger, D. A., Read, M. S., Brinkhous, K. M., et al. (1999) · Nature Medicine · doi:10.1038/4743 · Cited by 554 · see citing works
Davidson, B. L., Stein, C. S., Heth, J. A., Martins, I., Kotin, R. M., Derksen, T. A., Zabner, J., Ghodsi, A., & Chiorini, J. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3428 · Cited by 531 · see citing works
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells
Rizzuto, R., Brini, M., Pizzo, P., Murgia, M., & Pozzan, T. (1995) · Current Biology · doi:10.1016/s0960-9822(95)00128-x · Cited by 499 · see citing works
New recombinant serotypes of AAV vectors
Gao, G., Vandenberghe, L., & Wilson, J. (2005) · Current Gene Therapy · doi:10.2174/1566523054065057 · Cited by 487 · see citing works
McCown, T. J., Xiao, X., Li, J., Breese, G. R., & Samulski, R. J. (1996) · Brain Research · doi:10.1016/0006-8993(95)01488-8 · Cited by 450 · see citing works
Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus
Flannery, J. G., Zolotukhin, S., Vaquero, M. I., LaVail, M. M., Muzyczka, N., & Hauswirth, W. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6916 · Cited by 407 · see citing works
Self-citationThomas, C. E., Storm, T. A., Huang, Z., & Kay, M. A. (2004) · Journal of Virology · doi:10.1128/jvi.78.6.3110-3122.2004 · Cited by 358 · see citing works
Mandel, R. J., Spratt, S. K., Snyder, R. O., & Leff, S. E. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.14083 · Cited by 328 · see citing works
Self-citationComparative Analysis of Adeno-Associated Viral Vector Serotypes 1, 2, 5, 7, And 8 in Mouse Brain
Taymans, J.-M., Vandenberghe, L. H., Haute, C. V. D., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J. M., Debyser, Z., & Baekelandt, V. (2007) · Human Gene Therapy · doi:10.1089/hum.2006.178 · Cited by 305 · see citing works
Long-Term Clinical Improvement in MPTP-Lesioned Primates after Gene Therapy with AAV-hAADC
Bankiewicz, K. S., Forsayeth, J., Eberling, J. L., Sánchez‐Pernaute, R., Pivirotto, P., Bringas, J., Herscovitch, P., Carson, R. E., Eckelman, W. C., Reutter, B. W., et al. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.05.005 · Cited by 282 · see citing works
Intracellular trafficking of adeno-associated viral vectors
Ding, W., Zhang, L., Yan, Z., & Engelhardt, J. F. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302527 · Cited by 251 · see citing works
Peden, C. S., Burger, C., Muzyczka, N., & Mandel, R. J. (2004) · Journal of Virology · doi:10.1128/jvi.78.12.6344-6359.2004 · Cited by 222 · see citing works
Self-citationMandel, R. J., Rendahl, K. G., Spratt, S. K., Snyder, R. O., Cohen, L. K., & Leff, S. E. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-11-04271.1998 · Cited by 219 · see citing works
Self-citationKlein, R. L., Dayton, R. D., Leidenheimer, N. J., Jansen, K., Golde, T. E., & Zweig, R. M. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2005.10.008 · Cited by 200 · see citing works
Nakai, H., Storm, T. A., & Kay, M. A. (2000) · Journal of Virology · doi:10.1128/jvi.74.20.9451-9463.2000 · Cited by 194 · see citing works
Adeno-Associated Virus-Mediated Gene Transfer to the Brain: Duration and Modulation of Expression
Lo, W. D., Qu, G., Sferra, T. J., Clark, R., Chen, R., & Johnson, P. R. (1999) · Human Gene Therapy · doi:10.1089/10430349950018995 · Cited by 188 · see citing works
Xu, L., Daly, T. M., Gao, C., Flotte, T. R., Song, S., Byrne, B. J., Sands, M. S., & Ponder, K. P. (2001) · Human Gene Therapy · doi:10.1089/104303401300042500 · Cited by 171 · see citing works
Klein, R. L., Hamby, M. E., Gong, Y., Hirko, A. C., Wang, S., Hughes, J. A., King, M. A., & Meyer, E. M. (2002) · Experimental Neurology · doi:10.1006/exnr.2002.7942 · Cited by 160 · see citing works
Adeno-associated virus vectors: activity and applications in the CNS
Peel, A. L., & Klein, R. L. (2000) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(00)00183-7 · Cited by 157 · see citing works
Recombinant adeno-associated viral vectors as therapeutic agents to treat neurological disorders
Mandel, R. J., Manfredsson, F. P., Foust, K. D., Rising, A. C., Reimsnider, S., Nash, K. R., & Bürger, C. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2005.11.009 · Cited by 126 · see citing works
Self-citationRecombinant Adeno-Associated Viral Vectors in the Nervous System
Burger, C., Nash, K., & Mandel, R. J. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.781 · Cited by 110 · see citing works
Self-citationGerdes, C. A., Castro, M. G., & Lowenstein, P. R. (2000) · Molecular Therapy · doi:10.1006/mthe.2000.0140 · Cited by 102 · see citing works
Hauck, B., Zhao, W., High, K. A., & Xiao, W. (2004) · Journal of Virology · doi:10.1128/jvi.78.24.13678-13686.2004 · Cited by 98 · see citing works
Mandel, R. J., Snyder, R. O., & Leff, S. E. (1999) · Experimental Neurology · doi:10.1006/exnr.1999.7203 · Cited by 93 · see citing works
Self-citationStriatal Delivery of rAAV-hAADC to Rats with Preexisting Immunity to AAV
Sanftner, L. M., Suzuki, B. M., Doroudchi, M. M., Feng, L., McClelland, A., Forsayeth, J. R., & Cunningham, J. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2003.12.005 · Cited by 89 · see citing works
Leff, S. E., Spratt, S. K., Snyder, R. O., & Mandel, R. J. (1999) · Neuroscience · doi:10.1016/s0306-4522(98)00741-6 · Cited by 75 · see citing works
Self-citationDing, W., Yan, Z., Žák, R., Saavedra, M. T., Rodman, D. M., & Engelhardt, J. F. (2003) · Journal of Virology · doi:10.1128/jvi.77.13.7361-7366.2003 · Cited by 72 · see citing works
Bürger, C., Nguyen, F. N., Deng, J. V., & Mandel, R. J. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.08.031 · Cited by 70 · see citing works
Self-citationRapid development of genotypic resistance to lamivudine when combined with zidovudine in pregnancy
Clarke, J. R., Braganza, R., Mirza, A., Stainsby, C. M., Ait‐Khaled, M., Wright, A., Lyall, H., Parker, D., McClure, M. O., Weber, J. N., et al. (1999) · Journal of Medical Virology · doi:10.1002/(sici)1096-9071(199911)59:3 · Cited by 44 · see citing works
Mandel, R. J., Rendahl, K. G., Snyder, R. O., & Leff, S. E. (1999) · Experimental Neurology · doi:10.1006/exnr.1999.7159 · Cited by 38 · see citing works
Self-citationStructural proteins of adenovirus-associated virus: subspecies and their relatedness
McPherson, R. A., & Rose, J. A. (1983) · Journal of Virology · doi:10.1128/jvi.46.2.523-529.1983 · Cited by 24 · see citing works
Input Virion Proteins: Cryptic Targets of Antivector Immune Responses in Preimmunized Subjects
Lowenstein, P. R. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.05.019 · Cited by 22 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Comparative Analysis of Adeno-Associated Viral Vector Serotypes 1, 2, 5, 7, And 8 in Mouse Brain
Taymans, J.-M., Vandenberghe, L. H., Haute, C. V. D., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J. M., Debyser, Z., & Baekelandt, V. (2007) · Human Gene Therapy · doi:10.1089/hum.2006.178 · Cited by 305 · see citing works
Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation
Haery, L., Deverman, B. E., Matho, K. S., Cetin, A. H., Woodard, K., Cepko, C. L., Guerin, K. I., Rego, M. A., Ersing, I., Bächle, S. M., et al. (2019) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2019.00093 · Cited by 207 · see citing works
Klein, R. L., Dayton, R. D., Tatom, J. B., Henderson, K. M., & Henning, P. P. (2008) · Molecular Therapy · doi:10.1038/sj.mt.6300331 · Cited by 196 · see citing works
Rapid axonal sprouting and pruning accompany functional reorganization in primary visual cortex
Yamahachi, H., Marik, S. A., McManus, J. N. J., Denk, W., & Gilbert, C. D. (2009) · Neuron · doi:10.1016/j.neuron.2009.11.026 · Cited by 193 · see citing works
Oliveras-Salvá, M., Van der Perren, A., Casadei, N., Stroobants, S., Nuber, S., D’Hooge, R., Van den Haute, C., & Baekelandt, V. (2013) · Molecular Neurodegeneration · doi:10.1186/1750-1326-8-44 · Cited by 191 · see citing works
Fenno, L. E., Ramakrishnan, C., Kim, Y. S., Evans, K. E., Lo, M., Vesuna, S. S., Inoue, M., Cheung, K. Y. M., Yuen, E., Pichamoorthy, N., et al. (2020) · Neuron · doi:10.1016/j.neuron.2020.06.003 · Cited by 175 · see citing works
Lawlor, P. A., Bland, R. J., Mouravlev, A., Young, D., & During, M. J. (2009) · Molecular Therapy · doi:10.1038/mt.2009.170 · Cited by 161 · see citing works
Lowenstein, P. R., Mandel, R. J., Xiong, W.-D., Kroeger, K. M., & Castro, M. G. (2007) · Current Gene Therapy · doi:10.2174/156652307782151498 · Cited by 157 · see citing works
Self-citationCastle, M. J., Gershenson, Z. T., Giles, A. R., Holzbaur, E. L. F., & Wolfe, J. H. N. (2014) · Human Gene Therapy · doi:10.1089/hum.2013.189 · Cited by 156 · see citing works
Controlling AAV Tropism in the Nervous System with Natural and Engineered Capsids
Castle, M. J., Turunen, H. T., Vandenberghe, L. H., & Wolfe, J. H. (2016) · Methods in Molecular Biology · doi:10.1007/978-1-4939-3271-9_10 · Cited by 151 · see citing works
“AAV2 diffuses less readily through both the brain and spinal cord parenchyma when compared against other serotypes, and therefore mediates transduction over a smaller area [19, 28, 32, 33].”
BackgroundMcFarland, N. R., Fan, Z., Xu, K., Schwarzschild, M. A., Feany, M. Β., Hyman, B. T., & McLean, P. J. (2009) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/nen.0b013e3181a24b53 · Cited by 131 · see citing works
Viral vector-mediated overexpression of α-synuclein as a progressive model of Parkinson’s disease
Ulusoy, A., Decressac, M., Kirik, D., & Björklund, A. (2010) · Progress in brain research · doi:10.1016/s0079-6123(10)84005-1 · Cited by 130 · see citing works
A comprehensive multiscale framework for simulating optogenetics in the heart
Boyle, P. M., Williams, J. C., Ambrosi, C. M., Entcheva, E. G., & Trayanova, N. A. (2013) · Nature Communications · doi:10.1038/ncomms3370 · Cited by 125 · see citing works
Towne, C., Schneider, B. L., Kieran, D., Redmond, D. E., & Aebischer, P. (2010) · Gene Therapy · doi:10.1038/gt.2009.119 · Cited by 124 · see citing works
Manfredsson, F. P., Tümer, N., Erdös, B., Landa, T., Broxson, C. S., Sullivan, L. F., Rising, A. C., Foust, K. D., Zhang, Y., Muzyczka, N., et al. (2009) · Molecular Therapy · doi:10.1038/mt.2009.45 · Cited by 100 · see citing works
Self-citationCastle, M. J., Perlson, E., Holzbaur, E. L. F., & Wolfe, J. H. N. (2013) · Molecular Therapy · doi:10.1038/mt.2013.237 · Cited by 93 · see citing works
Striatal Overexpression of ΔFosB Reproduces Chronic Levodopa-Induced Involuntary Movements
Cao, X., Yasuda, T., Uthayathas, S., Watts, R. L., Mouradian, M. M., Mochizuki, H., & Papa, S. M. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.0252-10.2010 · Cited by 92 · see citing works
McFarland, N. R., Lee, J., Hyman, B. T., & McLean, P. J. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06010.x · Cited by 91 · see citing works
der Perren, A. V., Toelen, J., Carlon, M. S., den Haute, C. V., Coun, F., Heeman, B., Reumers, V., Vandenberghe, L. H., Wilson, J. M., Debyser, Z., et al. (2011) · Gene Therapy · doi:10.1038/gt.2010.179 · Cited by 88 · see citing works
Evading the immune response upon in vivo gene therapy with viral vectors.
Sack, B. K., & Herzog, R. W. (2009) · PubMed · Cited by 89 · see citing works
Dodiya, H. B., Bjorklund, T., Stansell III, J., Mandel, R. J., Kirik, D., & Kordower, J. H. (2010) · Molecular Therapy · doi:10.1038/mt.2009.216 · Cited by 86 · see citing works
Self-citationElMallah, M. K., Falk, D. J., Nayak, S., Federico, R. A., Sandhu, M. S., Poirier, A. E., Byrne, B. J., & Fuller, D. D. (2013) · Molecular Therapy · doi:10.1038/mt.2013.282 · Cited by 82 · see citing works
Gene Therapy in Parkinsonʼs Disease
Feng, L., & Maguire‐Zeiss, K. A. (2010) · CNS Drugs · doi:10.2165/11533740-000000000-00000 · Cited by 78 · see citing works
Snyder‐Keller, A., McLear, J. A., Hathorn, T., & Messer, A. C. (2010) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/nen.0b013e3181f530ec · Cited by 73 · see citing works
Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS
Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B., Bennett, A. D., Agbandje‐McKenna, M., Sortwell, C. E., Hauswirth, W. W., Boye, S. L., & Manfredsson, F. P. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.06.011 · Cited by 74 · see citing works
Self-citationVelde, G. V., Rangarajan, J. R., Toelen, J., Dresselaers, T., Ibrahimi, A., Krylychkina, O., Vreys, R., der Linden, A. V., Maes, F., Debyser, Z., et al. (2011) · Gene Therapy · doi:10.1038/gt.2011.2 · Cited by 71 · see citing works
Cunningham, J., Pivirotto, P., Bringas, J., Suzuki, B. M., Vijay, S., Sanftner, L. M., Kitamura, M., Chan, C. M., & Bankiewicz, K. S. (2008) · Molecular Therapy · doi:10.1038/mt.2008.111 · Cited by 68 · see citing works
Tight Long-term Dynamic Doxycycline Responsive Nigrostriatal GDNF Using a Single rAAV Vector
Manfredsson, F. P., Bürger, C., Rising, A. C., Zuobi-Hasona, K., Sullivan, L. F., Lewin, A. S., Huang, J. H., Piercefield, E. W., Muzyczka, N., & Mandel, R. J. (2009) · Molecular Therapy · doi:10.1038/mt.2009.196 · Cited by 67 · see citing works
Self-citationNew tools for investigating astrocyte-to-neuron communication
Li, D., Agulhon, C., Schmidt, E. M., Oheim, M., & Ropert, N. (2013) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2013.00193 · Cited by 66 · see citing works
Sellnow, R. C., Newman, J. H., Chambers, N., West, A. R., Steece‐Collier, K., Sandoval, I. M., Benskey, M. J., Bishop, C., & Manfredsson, F. P. (2019) · Acta Neuropathologica Communications · doi:10.1186/s40478-018-0653-7 · Cited by 64 · see citing works
Self-citationScheyltjens, I., Laramée, M., den Haute, C. V., Gijsbers, R., Debyser, Z., Baekelandt, V., Vreysen, S., & Arckens, L. (2015) · The Journal of Comparative Neurology · doi:10.1002/cne.23819 · Cited by 56 · see citing works
Mandel, R. J., Marmion, D. J., Kirik, D., Chu, Y., Heindel, C., McCown, T., Gray, S. J., & Kordower, J. H. (2017) · Acta Neuropathologica Communications · doi:10.1186/s40478-017-0451-7 · Cited by 56 · see citing works
Self-citationGorbatyuk, O. S., Li, S., Nguyen, F. N., Manfredsson, F. P., Kondrikova, G., Sullivan, L. F., Meyers, C., Chen, W., Mandel, R. J., & Muzyczka, N. (2010) · Molecular Therapy · doi:10.1038/mt.2010.137 · Cited by 52 · see citing works
Self-citationBenskey, M. J., Sandoval, I. M., & Manfredsson, F. P. (2016) · Human Gene Therapy Methods · doi:10.1089/hgtb.2015.117 · Cited by 49 · see citing works
Self-citationBjörklund, T., Carlsson, T., Cederfjäll, E. A., Carta, M., & Kirik, D. (2010) · Brain · doi:10.1093/brain/awp314 · Cited by 48 · see citing works
Benskey, M. J., Kuhn, N. C., Galligan, J. J., Garcia, J., Boye, S. L., Hauswirth, W. W., Mueller, C., Boye, S. L., & Manfredsson, F. P. (2015) · Molecular Therapy · doi:10.1038/mt.2015.7 · Cited by 47 · see citing works
Self-citationManfredsson, F. P., Okun, M. S., & Mandel, R. J. (2009) · Current Gene Therapy · doi:10.2174/156652309789753400 · Cited by 46 · see citing works
Self-citationPeden, C. S., Manfredsson, F. P., Reimsnider, S. K., Poirier, A. E., Burger, C., Muzyczka, N., & Mandel, R. J. (2009) · Molecular Therapy · doi:10.1038/mt.2008.284 · Cited by 44 · see citing works
Self-citationFulton, S. L., Mitra, S., Lepack, A. E., Martin, J. A., Stewart, A. F., Converse, J., Hochstetler, M. J., Dietz, D., & Maze, I. S. (2022) · Neuropsychopharmacology · doi:10.1038/s41386-022-01279-4 · Cited by 44 · see citing works
High Transgene Expression by Lentiviral Vectors Causes Maldevelopment of Purkinje Cells In Vivo
Sawada, Y., Kajiwara, G., Iizuka, A., Takayama, K., Shuvaev, А. N., Koyama, C., & Hirai, H. (2010) · The Cerebellum · doi:10.1007/s12311-010-0161-1 · Cited by 42 · see citing works
Mandel, R. J., Bürger, C., & Snyder, R. O. (2007) · Experimental Neurology · doi:10.1016/j.expneurol.2007.08.008 · Cited by 44 · see citing works
Self-citationChiu, T., Wang, M.-J., & Su, C. (2012) · Journal of Biomedical Science · doi:10.1186/1423-0127-19-45 · Cited by 41 · see citing works
Pronounced microgliosis and neurodegeneration in aged rats after tau gene transfer
Klein, R. L., Dayton, R. D., Diaczynsky, C. G., & Wang, D. B. (2009) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2008.12.002 · Cited by 39 · see citing works
A role for BDNF in cocaine reward and relapse
Schoenbaum, G. M., Stalnaker, T. A., & Shaham, Y. (2007) · Nature Neuroscience · doi:10.1038/nn0807-935 · Cited by 39 · see citing works
Qi, X., Guzhva, L., Yang, Z., Febo, M., Shan, Z. J., Wang, K., & Bruijnzeel, A. W. (2016) · European Neuropsychopharmacology · doi:10.1016/j.euroneuro.2016.07.007 · Cited by 39 · see citing works
Kou, J., Kim, H.-D., Pattanayak, A., Song, M., Lim, J.-E., Taguchi, H., Paul, S., Cirrito, J. R., Ponnazhagan, S., & Fukuchi, K.-I. (2011) · Journal of Alzheimer s Disease · doi:10.3233/jad-2011-110230 · Cited by 37 · see citing works
Cederfjäll, E. A., Şahin, G., Kirik, D., & Björklund, T. (2012) · Molecular Therapy · doi:10.1038/mt.2012.1 · Cited by 37 · see citing works
Bile diversion, a bariatric surgery, and bile acid signaling reduce central cocaine reward
Reddy, I. A., Smith, N. K., Erreger, K., Ghose, D., Saunders, C., Foster, D. J., Turner, B. D., Poe, A. M., Albaugh, V. L., McGuinness, O. P., et al. (2018) · PLoS Biology · doi:10.1371/journal.pbio.2006682 · Cited by 36 · see citing works
AAV1 is the optimal viral vector for optogenetic experiments in pigeons (Columba livia)
Rook, N., Tuff, J. M., Isparta, S., Masseck, O. A., Herlitze, S., Güntürkün, O., & Pusch, R. (2021) · Communications Biology · doi:10.1038/s42003-020-01595-9 · Cited by 36 · see citing works
Fischer, D. L., Manfredsson, F. P., Kemp, C. J., Cole-Strauss, A., Lipton, J. W., Duffy, M. F., Polinski, N. K., Steece‐Collier, K., Collier, T. J., Gombash, S. E., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-16690-x · Cited by 33 · see citing works
Self-citationGHS-R1a signaling in the DMH and VMH contributes to food anticipatory activity
Merkestein, M., van Gestel, M. A., van der Zwaal, E. M., Brans, M. A. D., Luijendijk, M. C. M., van Rozen, A. J., Hendriks, J., Garner, K. M., Boender, A. J., Pandit, R., et al. (2013) · International Journal of Obesity · doi:10.1038/ijo.2013.131 · Cited by 33 · see citing works
Gombash, S. E., Lipton, J. W., Collier, T. J., Madhavan, L., Steece‐Collier, K., Cole-Strauss, A., Terpstra, B. T., Spieles-Engemann, A. L., Daley, B. F., Wohlgenant, S. L., et al. (2011) · Molecular Therapy · doi:10.1038/mt.2011.216 · Cited by 33 · see citing works
Self-citationWu, K., Li, S., Bodhinathan, K., Meyers, C., Chen, W., Campbell‐Thompson, M., McIntyre, L. M., Foster, T. C., Muzyczka, N., & Kumar, A. (2011) · Neurobiology of Learning and Memory · doi:10.1016/j.nlm.2011.09.006 · Cited by 32 · see citing works
Self-citationMelanocortin receptor-mediated effects on obesity are distributed over specific hypothalamic regions
de Backer, M. W. A., la Fleur, S. E., Brans, M. A. D., van Rozen, A. J., Luijendijk, M. C. M., Merkestein, M., Garner, K. M., van der Zwaal, E. M., & Adan, R. A. H. (2010) · International Journal of Obesity · doi:10.1038/ijo.2010.169 · Cited by 33 · see citing works
Kneynsberg, A., Collier, T. J., Manfredsson, F. P., & Kanaan, N. M. (2016) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2016.03.004 · Cited by 31 · see citing works
Self-citationOptimization of Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus
de Backer, M. W. A., Brans, M. A. D., Luijendijk, M. C. M., Garner, K. M., & Adan, R. A. H. (2010) · Human Gene Therapy · doi:10.1089/hum.2009.169 · Cited by 30 · see citing works
Intraparenchymal Stereotaxic Delivery of rAAV and Special Considerations in Vector Handling
Benskey, M. J., & Manfredsson, F. P. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3271-9_14 · Cited by 28 · see citing works
Self-citationIn vivo methods for acute modulation of gene expression in the central nervous system
Cwetsch, A. W., Pinto, B., Savardi, A., & Cancedda, L. (2018) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2018.04.008 · Cited by 26 · see citing works
Cellular glycan modification by B3GAT1 broadly restricts influenza virus infection
Trimarco, J. D., Nelson, S. L., Chaparian, R. R., Wells, A. I., Murray, N. B., Azadi, P., Coyne, C. B., & Heaton, N. S. (2022) · Nature Communications · doi:10.1038/s41467-022-34111-0 · Cited by 25 · see citing works
Jiang, R., Haustein, M. D., Sofroniew, M. V., & Khakh, B. S. (2014) · Journal of Visualized Experiments · doi:10.3791/51972 · Cited by 25 · see citing works
Impact of age and vector construct on striatal and nigral transgene expression
Polinski, N. K., Manfredsson, F. P., Benskey, M. J., Fischer, D. L., Kemp, C. J., Steece‐Collier, K., Sandoval, I. M., Paumier, K. L., & Sortwell, C. E. (2016) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2016.82 · Cited by 25 · see citing works
Self-citationQi, X., Shan, Z. J., Ji, Y., Guerra, V., Alexander, J. C., Ormerod, B. K., & Bruijnzeel, A. W. (2014) · Translational Psychiatry · doi:10.1038/tp.2014.25 · Cited by 24 · see citing works
β1-Integrins in the Developing Orbitofrontal Cortex Are Necessary for Expectancy Updating in Mice
DePoy, L. M., Shapiro, L. P., Kietzman, H. W., Roman, K. M., & Gourley, S. L. (2019) · Journal of Neuroscience · doi:10.1523/jneurosci.3072-18.2019 · Cited by 20 · see citing works
Ojano-Dirain, C. P., Glushakova, L. G., Li, Z., Zolotukhin, S., Muzyczka, N., Srivastava, A., & Stacpoole, P. W. (2010) · Molecular Genetics and Metabolism · doi:10.1016/j.ymgme.2010.07.008 · Cited by 19 · see citing works
Self-citationGene Therapy for Misfolding Protein Diseases of the Central Nervous System
Sebastián, W. S., Samaranch, L., Kells, A. P., Forsayeth, J., & Bankiewicz, K. S. (2013) · Neurotherapeutics · doi:10.1007/s13311-013-0191-8 · Cited by 17 · see citing works
Gene transfer of integration defective anti-HSV-1 meganuclease to human corneas ex vivo
Elbadawy, H. M., Gailledrat, M., Desseaux, C., Salvalaio, G., Iorio, E. D., Ferrari, B., Bertolin, M., Barbaro, V., Parekh, M., Gayon, R., et al. (2014) · Gene Therapy · doi:10.1038/gt.2013.82 · Cited by 18 · see citing works
Hernandez, J. S., Binette, A. N., Rahman, T., Tarantino, J. D., & Moorman, D. E. (2020) · Alcoholism Clinical and Experimental Research · doi:10.1111/acer.14407 · Cited by 19 · see citing works
Doherty, F. C., Schaack, J. B., & Sladek, C. D. (2011) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2011.02.026 · Cited by 18 · see citing works
Recombinant Adeno-Associated Virus: Efficient Transduction of the Rat VMH and Clearance from Blood
van Gestel, M. A., Boender, A. J., de Vrind, V. A. J., Garner, K. M., Luijendijk, M. C. M., & Adan, R. A. H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0097639 · Cited by 18 · see citing works
Sanchez, C. E., Tierney, T. S., Gale, J. T., Alavian, K. N., Sahin, A., Lee, J.-S., Mulligan, R. C., & Carter, B. S. (2010) · Journal of neurosurgery · doi:10.3171/2010.8.jns091583 · Cited by 15 · see citing works
Yegla, B., & Parikh, V. (2017) · Behavioural Brain Research · doi:10.1016/j.bbr.2017.08.017 · Cited by 15 · see citing works
Bockstael, O., Melas, C., Pythoud, C., Levivier, M., McCarty, D. M., Samulski, R. J., Witte, O. D., & Tenenbaum, L. (2012) · Human Gene Therapy · doi:10.1089/hum.2011.216 · Cited by 14 · see citing works
Owusu-Yaw, B. S., Zhang, Y., Garrett, L., Yao, A., Shing, K., Batista, A. R., Sena‐Esteves, M., Upadhyay, J., Kegel-Gleason, K. B., & Todd, N. (2024) · Pharmaceutics · doi:10.3390/pharmaceutics16060710 · Cited by 13 · see citing works
Minnig, M. A., Park, T., Sanchez, M. E., Cottone, P., & Sabino, V. (2021) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2021.787362 · Cited by 11 · see citing works
de Backer, M. W. A., Fitzsimons, C. P., Brans, M. A. D., Luijendijk, M. C. M., Garner, K. M., Vreugdenhil, E., & Adan, R. A. H. (2010) · BMC Neuroscience · doi:10.1186/1471-2202-11-81 · Cited by 12 · see citing works
Dhanushkodi, A., Xue, Y., Roguski, E. E., Ding, Y., Matta, S. G., Heck, D. H., Fan, G.-H., & McDonald, M. P. (2018) · Neuroscience Letters · doi:10.1016/j.neulet.2018.10.038 · Cited by 13 · see citing works
A brief guide for gene delivery to the brain using adeno-associated viral vectors
Han, S., Yang, E. M., & Hur, E. (2025) · Molecules and Cells · doi:10.1016/j.mocell.2025.100189 · Cited by 11 · see citing works
The Degenerating Substantia Nigra as a Susceptible Region for Gene Transfer-Mediated Inflammation
Roca, V., Casabona, J. C., Radice, P. D., Murta, V., & Pitossi, F. J. (2011) · Parkinson s Disease · doi:10.4061/2011/931572 · Cited by 11 · see citing works
Chtarto, A., Bockstael, O., Gebara, E., Vermoesen, K., Melas, C., Pythoud, C., Levivier, M., Witte, O. D., Luthi‐Carter, R., Clinkers, R., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0053156 · Cited by 11 · see citing works
Yang, C., Hao, F., He, J., Lu, T., Klein, R. L., Zhao, L., & Duan, W. (2016) · Human Gene Therapy · doi:10.1089/hum.2015.083 · Cited by 11 · see citing works
Yang, C., Yang, W., Chen, S., Bao-feng, M., Li, B., Lu, T., Qu, T., Klein, R. L., Zhao, L., & Duan, W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0063876 · Cited by 10 · see citing works
Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction
Madrakhimov, S. B., Yang, J. Y., Ahn, D. H., Han, J. W., Ha, T. H., & Park, T. K. (2020) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2020.03.018 · Cited by 10 · see citing works
Recombinant Adeno-Associated Viral Vectors
de Backer, M. W. A., Garner, K. M., Luijendijk, M. C. M., & Adan, R. A. H. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-310-3_24 · Cited by 10 · see citing works
Gross, D., Ghenassia, A., Bartolo, L., Urbain, D., Benkhelifa‐Ziyyat, S., Lorain, S., Davoust, J. M., & Chappert, P. (2018) · Journal of Virology · doi:10.1128/jvi.01334-18 · Cited by 9 · see citing works
Luchicchi, A., Pattij, T., Viaña, J. N. M., de Kloet, S. F., & Marchant, N. J. (2020) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2020.109004 · Cited by 7 · see citing works
Stevens, L., Larsen, L. E., Lysebettens, W. V., Carrette, E., Boon, P., Raedt, R., & Vonck, K. E. J. (2021) · Frontiers in Neuroscience · doi:10.3389/fnins.2021.663337 · Cited by 8 · see citing works
Prospect of Induced Pluripotent Stem Cell Genetic Repair to Cure Genetic Diseases
Pawitan, J. A. (2012) · Stem Cells International · doi:10.1155/2012/498197 · Cited by 7 · see citing works
Zhang, Y., Gao, Y., Speth, R. C., Jiang, N., Mao, Y., Sumners, C., & Li, H. (2013) · International Journal of Medical Sciences · doi:10.7150/ijms.5700 · Cited by 7 · see citing works
Yang, P., Qin, Y., Zhang, W., Bian, Z., & Wang, R. L. (2015) · Journal of Molecular Neuroscience · doi:10.1007/s12031-015-0610-x · Cited by 7 · see citing works
Adeno-associated virus-mediated ILK gene silencing in the rat NAc core
Chen, Q., Xiong, X., Lee, T. H., Liu, Y., Sun, Q.-A., Wetsel, W. C., & Zhang, X. (2008) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2008.06.004 · Cited by 7 · see citing works
Daw, T. B., El-Nahal, H. G., Basso, M. A., Jun, E. J., Bautista, A. R., Samulski, R. J., Sommer, M. A., & Bohlen, M. O. (2023) · Human Gene Therapy · doi:10.1089/hum.2022.194 · Cited by 6 · see citing works
Thiele, N., Hildebrandt, K. J., & Köppl, C. (2020) · Current Research in Neurobiology · doi:10.1016/j.crneur.2020.100001 · Cited by 6 · see citing works
Masri, S., Mowery, T. M., Fair, R., & Sanes, D. H. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2311570121 · Cited by 6 · see citing works
Optogenetic stimulation of a cortical biohybrid implant guides goal directed behaviorPreprint
Brown, J., Zappitelli, K. M., Dawson, P. M., Yoon, E., Shiraga, S., Rochford, A. E., Eltaeb, M., Rodriguez, A., Kong, Y., Hodak, M., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.22.624907 · Cited by 6 · see citing works
von Hövel, F. F., Rumpel, R., Ratzka, A., Schreiner, D., & Grothe, C. (2019) · Cell and Tissue Research · doi:10.1007/s00441-019-03013-x · Cited by 5 · see citing works
Zhu, Q.-X., Zhang, Y., Zhang, H., Leng, C., Deng, C.-L., Wang, X., Li, J.-J., Ye, X.-L., Zhang, B., & Li, X. (2024) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0012604 · Cited by 5 · see citing works
Chronic Two-Photon Imaging of Neural Activity in the Anesthetized and Awake Behaving Rodent
Margolis, D. J., Lütcke, H., Helmchen, F., Weber, B., & Haiss, F. (2013) · Neuromethods · doi:10.1007/978-1-62703-785-3_10 · Cited by 5 · see citing works
Microarray profiling of hypothalamic gene expression changes in Huntington’s disease mouse models
Dickson, E., Dwijesha, A. S., Andersson, N., Lundh, S. H., Björkqvist, M., Petersén, Å., & Soylu-Kucharz, R. (2022) · Frontiers in Neuroscience · doi:10.3389/fnins.2022.1027269 · Cited by 5 · see citing works
McManus, G., Centner, A., Budrow, C., Lipari, N., Tseng, K. Y., Manfredsson, F. P., & Bishop, C. (2024) · Neuropharmacology · doi:10.1016/j.neuropharm.2024.110227 · Cited by 4 · see citing works
Self-citationUsing Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain
der Perren, A. V., Toelen, J., Taymans, J., & Baekelandt, V. (2011) · Neuromethods · doi:10.1007/978-1-61779-533-6_3 · Cited by 4 · see citing works
- Authors
- Reimsnider, S., Manfredsson, F. P., Muzyczka, N., & Mandel, R. J.
- Affiliations
- University of Florida Health (US)
- Corresponding author
- Mandel, Ronald J
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2007-08
- Volume
- 15
- Issue
- 8
- Pages
- 1504-1511
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W1983678138
- Cited by
- 125
- References
- 42
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 3 · 2021: 4 · 2020: 7 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 5 · 2014: 5 · 2013: 11 · 2012: 4
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Therapy (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- University of Florida
- National Institute of Neurological Disorders and Stroke (P01NS036302, P01NS36302)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- recombinant adeno-associated virus vectors, intrastriatal injection, striatal neurons, green fluorescent protein, neuroinflammation, gene therapy
- MeSH terms
- Adenoviridae, Animals, Cell Differentiation, DNA, Recombinant, Female, Genetic Vectors, Neuroglia, Time Factors, Transduction, Genetic, Gene Expression, Rats, Sprague-Dawley, Genes, Reporter, Transgenes, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review