A High-Fat High-Sucrose Diet Rapidly Alters Muscle Integrity, Inflammation and Gut Microbiota in Male Rats
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The chronic low-level inflammation associated with obesity is known to deleteriously affect muscle composition. However, the manner in which obesity leads to muscle loss has not been explored in detail or in an integrated manner following a short-term metabolic challenge. In this paper, we evaluated the relationships between compromised muscle integrity, diet, systemic inflammatory mediators, adipose tissue, and gut microbiota in male Sprague-Dawley rats. We show that intramuscular fat, fibrosis, and the number of pro-inflammatory cells increased by 3-days and was sustained across 28-days of high-fat high-sugar feeding compared to control-diet animals. To understand systemic contributors to muscle damage, dynamic changes in gut microbiota and serum inflammatory markers were evaluated. Data from this study links metabolic challenge to persistent compromise in muscle integrity after just 3-days, a finding associated with altered gut microbiota and systemic inflammatory changes. These data contribute to our understanding of early consequences of metabolic challenge on multiple host systems, which are important to understand as obesity treatment options are developed. Therefore, intervention within this early period of metabolic challenge may be critical to mitigate these sustained alterations in muscle integrity.
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In the same rats, there was an increase in circulating inflammatory cytokines (IL-6, TNF-α, and MCP-1) and intramuscular fat after just 3 days.
“the authors reported an increase in circulating inflammatory cytokines (IL-6, TNF-α, and MCP-1) and intramuscular fat after just 3 days [85]”
From Gut Microbiota Contribute to Age-Related Changes in Skeletal Muscle Size, Composition, and Function: Biological Basis for a Gut-Muscle Axis (2018) · cites this paper as [85]
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- Authors
- Collins, K. H., Paul, H. A., Hart, D. A., Reimer, R. A., Smith, I. C., Rios, J. L., Seerattan, R. A., & Herzog, W.
- Affiliations
- University of Calgary (CA)
- University of British Columbia (CA)
- Corresponding author
- Herzog, Walter
- ORCID
- Venue
- Scientific Reports
- Published
- 2016-11-17
- Volume
- 6
- Issue
- 1
- Pages
- 37278-37278
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5112513
- OpenAlex
- W2549601621
- Cited by
- 125
- References
- 37
- Citations by year
- 2026: 8 · 2025: 12 · 2024: 16 · 2023: 9 · 2022: 12 · 2021: 14 · 2020: 17 · 2019: 13 · 2018: 14 · 2017: 10
- FWCI
- 6.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (13157-13-2)
- Killam Trusts
- Ministério da Educação
- Alberta Innovates
- Canadian Institutes of Health Research (36475_1, MOP115076)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- high-fat high-sugar diet, obesity, muscle integrity, muscle damage, intramuscular fat, muscle fibrosis, systemic inflammation, gut microbiota, adipose tissue, metabolic challenge, short-term dietary intervention
- MeSH terms
- Gastrointestinal Microbiome, Animals, Dietary Carbohydrates, Dietary Fats, Male, Rats, Sprague-Dawley, Muscle, Skeletal, Rats
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