Cited by 132Adipose Tissue and MetabolismMuscle Physiology and DisordersMetabolism, Diabetes, and Cancer

Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice

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de Wilde, Janneke, Mohren, Ronny, van den Berg, Sjoerd, Boekschoten, Mark, Dijk, Ko Willems-Van, de Groot, Philip, Müller, Michael, Mariman, Edwin +1 more·2008·Physiological Genomics

Abstract

The prevalence of the metabolic syndrome (MS) is rapidly increasing all over the world. Consequently, there is an urgent need for more effective intervention strategies. Both animal and human studies indicate that lipid oversupply to skeletal muscle can result in insulin resistance, which is one of the characteristics of the MS. C57BL/6J mice were fed a low-fat (10 kcal%) palm oil diet or a high-fat (45 kcal%; HF) palm oil diet for 3 or 28 days. By combining transcriptomics with protein and lipid analyses we aimed to better understand the molecular events underlying the early onset of the MS. Short-term HF feeding led to altered expression levels of genes involved in a variety of biological processes including morphogenesis, energy metabolism, lipogenesis, and immune function. Protein analysis showed increased levels of the myosin heavy chain, slow fiber type protein, and the complexes I, II, III, IV, and V of the oxidative phosphorylation. Furthermore, we observed that the main mitochondrial membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, contained more saturated fatty acids. Altogether, these results point to a morphological as well as a metabolic adaptation by promoting a more oxidative fiber type. We hypothesize that after this early positive adaptation, a continued transcriptional downregulation of genes involved in oxidative phosphorylation will result in decreased oxidative capacity at a later stage. Together with increased saturation of phospholipids of the mitochondrial membrane this can result in decreased mitochondrial function, which is a hallmark observed in insulin resistance and Type 2 diabetes.

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Crossref · 10.1152/physiolgenomics.00219.2007

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Authors
de Wilde, J., Mohren, R., van den Berg, S., Boekschoten, M., Dijk, K. W.-V., de Groot, P., Müller, M., Mariman, E., & Smit, E.
Affiliations
  • Nutrigenomics Consortium, Top Institute Food and Nutrition, Wageningen
  • Maastricht University (NL)
  • Wageningen University & Research (NL)
  • Leiden University Medical Center (NL)
Venue
Physiological Genomics
Published
2008-02
Volume
32
Issue
3
Pages
360-369
ISSN
  • 1094-8341 (ISSN-L)
  • 1531-2267
OpenAlex
W2132226748
Cited by
132
References
71
Citations by year
2026: 1 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 3 · 2019: 8 · 2018: 9 · 2017: 16 · 2016: 6 · 2015: 11 · 2014: 5 · 2013: 9 · 2012: 9
FWCI
4.90
Type
journal article
Language
en
Open access
Locations
  • Physiological Genomics (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion)
  • Pure Amsterdam UMC (publishedVersion)
  • Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • UEA Digital Repository (University of East Anglia) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
high-fat diet, skeletal muscle, metabolic syndrome, insulin resistance, type 2 diabetes, oxidative phosphorylation, mitochondrial membrane phospholipids, phosphatidylcholine, phosphatidylethanolamine, myosin heavy chain, lipogenesis, energy metabolism, gene expression, C57BL/6J mice
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Palm Oil, Adaptation, Physiological, Animals, Blood Glucose, Dietary Fats, Unsaturated, Fatty Acids, Gene Expression Regulation, Glucose, Insulin, Male, Mice, Inbred C57BL, Muscle Proteins, Oxidation-Reduction, Plant Oils, Random Allocation, Transcription Factors, Trans-Activators, Muscle, Skeletal, Muscle Fibers, Slow-Twitch, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Metabolic Syndrome, Mice

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