Differential gene body methylation and reduced expression of cell adhesion and neurotransmitter receptor genes in adverse maternal environment
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Early life adversity, including adverse gestational and postpartum maternal environment, is a contributing factor in the development of autism, attention deficit hyperactivity disorder (ADHD), anxiety and depression but little is known about the underlying molecular mechanism. In a model of gestational maternal adversity that leads to innate anxiety, increased stress reactivity and impaired vocal communication in the offspring, we asked if a specific DNA methylation signature is associated with the emergence of the behavioral phenotype. Genome-wide DNA methylation analyses identified 2.3% of CpGs as differentially methylated (that is, differentially methylated sites, DMSs) by the adverse environment in ventral-hippocampal granule cells, neurons that can be linked to the anxiety phenotype. DMSs were typically clustered and these clusters were preferentially located at gene bodies. Although CpGs are typically either highly methylated or unmethylated, DMSs had an intermediate (20-80%) methylation level that may contribute to their sensitivity to environmental adversity. The adverse maternal environment resulted in either hyper or hypomethylation at DMSs. Clusters of DMSs were enriched in genes that encode cell adhesion molecules and neurotransmitter receptors; some of which were also downregulated, indicating multiple functional deficits at the synapse in adversity. Pharmacological and genetic evidence links many of these genes to anxiety.
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Oh et al. (176) found that adverse maternal environment induced DNA methylation changes in the offspring and the affected genes encode proteins involved in synapse formation and function (reduced expression of cell adhesion and neurotransmitter receptor genes was observed).
“the authors found that adverse maternal environment induced DNA methylation changes in the offspring and the affected genes encode proteins involved in synapse formation and function (reduced expression of cell adhesion and neurotransmitter receptor genes was observed).”
From Gene × Environment Interactions in Autism Spectrum Disorders: Role of Epigenetic Mechanisms (2014) · cites this paper as [176]
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- Authors
- Oh, J.-E., Chambwe, N., Klein, S., Gal, J., Andrews, S., Gleason, G., Shaknovich, R., Melnick, A., Campagne, F., & Toth, M.
- Affiliations
- Weill Cornell Medicine (US)
- Rockefeller University (US)
- Corresponding author
- Oh, J-e
- Venue
- Translational Psychiatry
- Published
- 2013-01-22
- Volume
- 3
- Issue
- 1
- Pages
- e218-e218
- ISSN
- 2158-3188 (ISSN-L)
- PMC
- PMC3566713
- OpenAlex
- W2118987066
- Cited by
- 43
- References
- 69
- Citations by year
- 2024: 1 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 7 · 2015: 8 · 2014: 5 · 2013: 1
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- www.springer.com/tdm (unspecified)
- Locations
- Translational Psychiatry (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (R01MH058669, R01MH086883, 1R01-MH58669, R01MH094720)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Maternal Mental Health During Pregnancy and Postpartum
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Keywords
- childhood adversity, DNA methylation, gene body methylation, differentially methylated sites, ventral hippocampus, granule cells, anxiety, adhesion molecules, neurotransmitter receptors, synapses, stress reactivity, vocal communication
- MeSH terms
- Animals, Anxiety, Cell Adhesion, Disease Models, Animal, Female, Male, Mice, Transgenic, Pregnancy, Prenatal Exposure Delayed Effects, Vocalization, Animal, Dentate Gyrus, CpG Islands, DNA Methylation, Epigenesis, Genetic, Receptor, Serotonin, 5-HT1A, Mice
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