The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Perfused multiwell plate for 3D liver tissue engineering
Open accessAbstract
In vitro models that capture the complexity of in vivo tissue and organ behaviors in a scalable and easy-to-use format are desirable for drug discovery. To address this, we have developed a bioreactor that fosters maintenance of 3D tissue cultures under constant perfusion and we have integrated multiple bioreactors into an array in a multiwell plate format. All bioreactors are fluidically isolated from each other. Each bioreactor in the array contains a scaffold that supports formation of hundreds of 3D microscale tissue units. The tissue units are perfused with cell culture medium circulated within the bioreactor by integrated pneumatic diaphragm micropumps. Electronic controls for the pumps are kept outside the incubator and connected to the perfused multiwell by pneumatic lines. The docking design and open-well bioreactor layout make handling perfused multiwell plates similar to using standard multiwell tissue culture plates. A model of oxygen consumption and transport in the circulating culture medium was used to predict appropriate operating parameters for primary liver cultures. Oxygen concentrations at key locations in the system were then measured as a function of flow rate and time after initiation of culture to determine oxygen consumption rates. After seven days of culture, tissue formed from cells seeded in the perfused multiwell reactor remained functionally viable as assessed by immunostaining for hepatocyte and liver sinusoidal endothelial cell (LSEC) phenotypic markers.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Hewitt, N. J., Gómez Lechón, M. J., Houston, J. B., Hallifax, D., Brown, H. S., Maurel, P., Kenna, J. G., Gustavsson, L., Lohmann, C., Skonberg, C., et al. (2007) · Drug Metabolism Reviews · doi:10.1080/03602530601093489 · Cited by 735
Lee, P. J., Hung, P. J., & Lee, L. P. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21360 · Cited by 509
Engineered Microenvironments for Controlled Stem Cell Differentiation
Burdick, J. A., & Vunjak-Novakovic, G. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0131 · Cited by 485
A microfabricated array bioreactor for perfused 3D liver culture
Powers, M. J., Domansky, K., Kaazempur‐Mofrad, M. R., Kalezi, A., Capitano, A., Upadhyaya, A., Kurzawski, P., Wack, K. E., Stolz, D. B., Kamm, R., et al. (2002) · Biotechnology and Bioengineering · doi:10.1002/bit.10143 · Cited by 462
Self-citationA practical guide to microfluidic perfusion culture of adherent mammalian cells
Kim, L., Toh, Y.-C., Voldman, J., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b704602b · Cited by 455
Oxygen: Modulator of metabolic zonation and disease of the liver
Jungermann, K., & Kietzmann, T. (2000) · Hepatology · doi:10.1002/hep.510310201 · Cited by 443
Sung, J. H., & Shuler, M. L. (2009) · Lab on a Chip · doi:10.1039/b901377f · Cited by 431
A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b614872g · Cited by 414
Sin, A., Chin, K. C., Jamil, M. F., Kostov, Y., Rao, G., & Shuler, M. L. (2008) · Biotechnology Progress · doi:10.1021/bp034077d · Cited by 368
Formation of steady‐state oxygen gradients in vitro: Application to liver zonation
Allen, J. W., & Bhatia, S. N. (2003) · Biotechnology and Bioengineering · doi:10.1002/bit.10569 · Cited by 269
Cited by
Bhatia, S. N., & Ingber, D. E. (2014) · Nature Biotechnology · doi:10.1038/nbt.2989 · Cited by 3,305
Overcoming the challenges in administering biopharmaceuticals: Formulation and delivery strategies
Mitragotri, S., Burke, P. A., & Langer, R. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4363 · Cited by 1,702
Human organs-on-chips for disease modelling, drug development and personalized medicine
Ingber, D. E. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00466-9 · Cited by 1,589
A guide to the organ-on-a-chip
Leung, C. M., de Haan, P., Ronaldson-Bouchard, K., Kim, G.-A., Ko, J., Rho, H. S., Chen, Z., Habibovic, P., Jeon, N. L., Takayama, S., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00118-6 · Cited by 1,116
A Human Disease Model of Drug Toxicity–Induced Pulmonary Edema in a Lung-on-a-Chip Microdevice
Huh, D., Leslie, D. C., Matthews, B. D., Fraser, J. P., Jurek, S., Hamilton, G. A., Thorneloe, K. S., McAlexander, M. A., & Ingber, D. E. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004249 · Cited by 993
Metadata
- Authors
- Domansky, K., Inman, W., Serdy, J., Dash, A., Lim, M. H. M., & Griffith, L. G.
- Venue
- Lab Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2010
- Volume
- 10
- Issue
- 1
- Pages
- 51-58
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W1517911220
- Cited by
- 444
- References
- 27
- FWCI
- 12.03
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Perfused multiwell plate for 3D liver tissue engineering. https://app.cochat.ai/papers/10.1039/b913221j
- The paper
- Domansky, K., Inman, W., Serdy, J., Dash, A., Lim, M. H. M., & Griffith, L. G. (2010). Perfused multiwell plate for 3D liver tissue engineering. Lab Chip. https://doi.org/10.1039/b913221j
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
