Microfluidic platforms for mechanobiology
Open accessAbstract
Mechanotransduction has been a topic of considerable interest since early studies demonstrated a link between mechanical force and biological response. Until recently, studies of fundamental phenomena were based either on in vivo experiments with limited control or direct access, or on large-scale in vitro studies lacking many of the potentially important physiological factors. With the advent of microfluidics, many of the previous limitations of in vitro testing were eliminated or reduced through greater control or combined functionalities. At the same time, imaging capabilities were tremendously enhanced. In this review, we discuss how microfluidics has transformed the study of mechanotransduction. This is done in the context of the various cell types that exhibit force-induced responses and the new biological insights that have been elucidated. We also discuss new microfluidic studies that could produce even more realistic models of in vivo conditions by combining multiple stimuli or creating a more realistic microenvironment.
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These microfluidic systems enable continuous delivery of oxygen, nutrients, and therapeutic agents while introducing shear stress and directional mass transport, allowing investigation of flow-regulated processes within a tumor-relevant architecture.
“These systems enable continuous delivery of oxygen, nutrients, and therapeutic agents while introducing shear stress and directional mass transport, thereby allowing the investigation of flow-regulated processes within a tumor-relevant architecture [224,225,226,227,228].”
From Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis (2025) · cites this paper as [224]
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Micro- and nanoengineering for stem cell biology: the promise with a caution
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Atomic Force Microscopy Reveals Important Differences in Axonal Resistance to Injury
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Engineering of a microfluidic cell culture platform embedded with nanoscale features
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Single-molecule imaging of NGF axonal transport in microfluidic devices
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A new mechanobiological era: microfluidic pathways to apply and sense forces at the cellular level
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A multishear microfluidic device for quantitative analysis of calcium dynamics in osteoblasts
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Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells
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RhoA mediates flow-induced endothelial sprouting in a 3-D tissue analogue of angiogenesis
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A new chemotaxis device for cell migration studies
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Microfluidics for Applications in Cell Mechanics and Mechanobiology
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Dynamic osmotic loading of chondrocytes using a novel microfluidic device
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Biological cardio-micro-pumps for microbioreactors and analytical micro-systems
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Microfluidic devices for mechanical characterisation of single cells in suspension
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Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers
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Micropatterned Structural Control Suppresses Mechanotaxis of Endothelial Cells
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The membrane chamber: A new type of in vitro recording chamber
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Augmented stress-responsive characteristics of cell lines in narrow confinements
Das, T., Maiti, T. K., & Chakraborty, S. K. (2011) · Integrative Biology · doi:10.1039/c1ib00001b · Cited by 20 · see citing works
A microfluidic system for fast detection of mitochondrial DNA deletion
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Cell Structure Controls Endothelial Cell Migration under Fluid Shear Stress
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Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
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Microfluidic 3D cell culture: from tools to tissue models
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“[9 ].”
Organ-on-a-chip platforms: a convergence of advanced materials, cells, and microscale technologies
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“For more details on how microfluidic tools can be used to study mechanobiology we refer to a comprehensive review (Polacheck et al., 2013).”
3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate
Ma, Y., Lin, M., Huang, G., Li, Y., Wang, S., Bai, G., Lu, T. J., & Xu, F. (2018) · Advanced Materials · doi:10.1002/adma.201705911 · Cited by 230 · see citing works
Microfluidics: A New Tool for Modeling Cancer–Immune Interactions
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Dynamically tunable cell culture platforms for tissue engineering and mechanobiology
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“These tissue-dependent mechanical stimuli ultimately dictate cellular function and fate [36].”
BackgroundInfluentialHigh-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology
Yang, L., Pijuan‐Galito, S., Rho, H. S., Vasilevich, A. S., Eren, A. D., Ge, L., Habibović, P., Alexander, M. R., de Boer, J., Carlier, A., et al. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00752 · Cited by 187 · see citing works
Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices
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“The in egr t on of microfluidic in c ll culture provides an in ivo like platform to regulate the mechanobiological respons s on chips [77].”
BackgroundMethodMechanical Stimulation: A Crucial Element of Organ-on-Chip Models
Thompson, C. L., Fu, S., Heywood, H. K., Knight, M. M., & Thorpe, S. D. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.602646 · Cited by 156 · see citing works
“The use of microfluidic platforms such as OOC systems for the study of mechanobiology is well established (Polacheck et al., 2013), while the innovative OOC field is producing novel approaches toward incorporating biomechanical cues into chip design as recently reviewed by Kaarj and Yoon (2019).”
MethodIn vivo–mimicking microfluidic perfusion culture of pancreatic islet spheroids
Jun, Y., Lee, J., Choi, S., Yang, J. H., Sander, M., Chung, S. H., & Lee, S. (2019) · Science Advances · doi:10.1126/sciadv.aax4520 · Cited by 153 · see citing works
Flow-induced stress on adherent cells in microfluidic devices
Shemesh, J., Jalilian, I., Shi, A., Heng Yeoh, G., Knothe Tate, M. L., & Ebrahimi Warkiani, M. (2015) · Lab on a Chip · doi:10.1039/c5lc00633c · Cited by 144 · see citing works
Methods of Delivering Mechanical Stimuli to Organ-on-a-Chip
Kaarj, K., & Yoon, J. (2019) · Micromachines · doi:10.3390/mi10100700 · Cited by 140 · see citing works
“Syringe pump Connective tissue (fibroblasts) Effect of static strain on cellular alignment of fibroblasts encapsulated in hydrogel [45]”
Mechanical properties of single cells: Measurement methods and applications
Hao, Y., Cheng, S., Tanaka, Y., Hosokawa, Y., Yalikun, Y., & Li, M. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107648 · Cited by 132 · see citing works
Microengineered cancer-on-a-chip platforms to study the metastatic microenvironment
Lara, R. P., & Annabi, N. (2016) · Lab on a Chip · doi:10.1039/c6lc00718j · Cited by 128 · see citing works
Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models
Moreno-Arotzena, O., Meier, J. G., Amo, C. D., & García‐Aznar, J. M. (2015) · Materials · doi:10.3390/ma8041636 · Cited by 120 · see citing works
“Novel methodologies based on microfluidic techniques are being developed that offer unique features for the rational design of physiologically relevant in vitro systems [10], which could be directed to mimic wound healing processes.”
BackgroundHuber, D., Oskooei, A., Solvas, X. C. I., deMello, A. J., & Kaigala, G. V. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00317 · Cited by 125 · see citing works
Engineering and evaluating drug delivery particles in microfluidic devices
Björnmalm, M., Yan, Y., & Caruso, F. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.04.030 · Cited by 121 · see citing works
“This includes mechanobiological aspects of the in vivo setting and the impact of particle geometry and mechanics [93, 94].”
BackgroundShou, Y., Teo, X. Y., Wu, K. Z., Bai, B., Kumar, A. R. K., Low, J., Le, Z., & Tay, A. K. P. (2023) · Advanced Science · doi:10.1002/advs.202300670 · Cited by 117 · see citing works
Biomechanical Strain Exacerbates Inflammation on a Progeria‐on‐a‐Chip Model
Ribas, J., Zhang, Y. S., Pitrez, P. R., Leijten, J., Miscuglio, M., Rouwkema, J., Dokmeci, M. R., Nissan, X., Ferreira, L., & Khademhosseini, A. (2017) · Small · doi:10.1002/smll.201603737 · Cited by 115 · see citing works
Microfluidic Tissue Engineering and Bio‐Actuation
Filippi, M., Buchner, T., Yaşa, Ö., Weirich, S., & Katzschmann, R. K. (2022) · Advanced Materials · doi:10.1002/adma.202108427 · Cited by 111 · see citing works
Vascular endothelial cell mechanosensing: New insights gained from biomimetic microfluidic models
Gray, K. M., & Stroka, K. M. (2017) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2017.06.002 · Cited by 109 · see citing works
Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform
Uzel, S. G. M., Amadi, O. C., Pearl, T. M., Lee, R. T., So, P. T. C., & Kamm, R. D. (2016) · Small · doi:10.1002/smll.201501905 · Cited by 100 · see citing works
High-Throughput Microfluidic 3D Cytotoxicity Assay for Cancer Immunotherapy (CACI-IMPACT Platform)
Park, D., Son, K., Hwang, Y., Ko, J., Lee, Y., Doh, J., & Jeon, N. L. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.01133 · Cited by 96 · see citing works
“Microfluidic-based platforms, which allow the reconstitution of complex 3D microenvironments of human tissues in in vitro by compartmentalization of multiple cell types, applying chemical and mechanical stimulations, and controlling chemical gradient (18), can be a powerful method for the assessment of lymphocyte cyto…”
MethodStrategies for improving the physiological relevance of human engineered tissues
Abbott, R. D., & Kaplan, D. L. (2015) · Trends in biotechnology · doi:10.1016/j.tibtech.2015.04.003 · Cited by 88 · see citing works
Controlling Differentiation of Stem Cells for Developing Personalized Organ‐on‐Chip Platforms
Geraili, A., Jafari, P., Hassani, M. S., Araghi, B. H., Mohammadi, M. H., Ghafari, A. M., Tamrin, S. H., Modarres, H. P., Kolahchi, A. R., Ahadian, S., et al. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700426 · Cited by 86 · see citing works
Biomechanical Characterization at the Cell Scale: Present and Prospects
Basoli, F., Giannitelli, S. M., Gori, M., Mozetic, P., Bonfanti, A., Trombetta, M., & Rainer, A. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.01449 · Cited by 88 · see citing works
“Recent advances in the development of novel techniques and tools for cell mechanics characterization, and the design of ad hoc technological platforms have introduced the possibility to accurately trap and manipulate single cells at the microscale level (Finer et al., 1994; Park et al., 2005; Roth et al., 2013; Guo et…”
Nichols, J. E., Niles, J. A., Vega, S. P., Argueta, L. B., Eastaway, A., & Cortiella, J. (2014) · Experimental Biology and Medicine · doi:10.1177/1535370214536679 · Cited by 87 · see citing works
Probing Cell Deformability via Acoustically Actuated Bubbles
Xie, Y., Nama, N., Li, P., Mao, Z., Huang, P., Zhao, C., Costanzo, F., & Huang, T. J. (2015) · Small · doi:10.1002/smll.201502220 · Cited by 85 · see citing works
New advances in probing cell–extracellular matrix interactions
Liu, A. P., Chaudhuri, O., & Parekh, S. H. (2017) · Integrative Biology · doi:10.1039/c6ib00251j · Cited by 75 · see citing works
Influence of the microenvironment on cell fate determination and migration
Bloom, A. B., & Zaman, M. H. (2014) · Physiological Genomics · doi:10.1152/physiolgenomics.00170.2013 · Cited by 77 · see citing works
Pulsed Laser Activated Cell Sorting with Three Dimensional Sheathless Inertial Focusing
Chen, Y., Chung, A. J., Wu, T., Teitell, M. A., Carlo, D. D., & Chiou, P.-Y. E. (2014) · Small · doi:10.1002/smll.201302885 · Cited by 77 · see citing works
For whom the cells pull: Hydrogel and micropost devices for measuring traction forces
Ribeiro, A. J. S., Denisin, A. K., Wilson, R. E., & Pruitt, B. L. (2015) · Methods · doi:10.1016/j.ymeth.2015.08.005 · Cited by 72 · see citing works
“Figures adapted with permission from [144].”
Probing cell–cell communication with microfluidic devices
Guo, F., French, J. B., Li, P., Zhao, H., Chan, C. Y., Fick, J. R., Benkovic, S. J., & Huang, T. J. (2013) · Lab on a Chip · doi:10.1039/c3lc90067c · Cited by 73 · see citing works
Improved cell adhesion under shear stress in PDMS microfluidic devices
Siddique, A., Meckel, T., Stark, R. W., & Narayan, S. (2016) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2016.11.011 · Cited by 73 · see citing works
“The integration of microfluidics and biology has not only revoluionized the basics of in vitro cell culturing but has also enabled the evelopment of valuable tools for the investigation of mechanobilogical processes [1].”
MethodLuo, Y., Chen, D. Y., Zhao, Y., Wei, C. L., Zhao, X., Yue, W., Long, R., Wang, J., & Chen, J. (2014) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2014.05.028 · Cited by 72 · see citing works
Silk-microfluidics for advanced biotechnological applications: A progressive review
Konwarh, R., Gupta, P., & Mandal, B. B. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.05.001 · Cited by 71 · see citing works
Ravichandran, A., Liu, Y., & Teoh, S. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2270 · Cited by 68 · see citing works
“While the different mechanical stimuli experienced by tissues has been summarized before (Polacheck et al., 2013), this could be difficult to benchmark due to variations between anatomical differences physiologically and also different experimental set-ups between research studies.”
BackgroundShah, S., & D’Souza, G. G. M. (2025) · Cells · doi:10.3390/cells14100732 · Cited by 66 · see citing works
“These systems enable continuous delivery of oxygen, nutrients, and therapeutic agents while introducing shear stress and directional mass transport, thereby allowing the investigation of flow-regulated processes within a tumor-relevant architecture [224–228].”
Paggi, C. A., Venzac, B., Karperien, M., Leijten, J., & Gac, S. L. (2020) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2020.127917 · Cited by 66 · see citing works
“provides exquisite control on a cell microenvironment and allows studying in situ the impact of mechanical stimulations on the cell and organ function, as reviewed by the Kamm group [16].”
BackgroundWeiss, A. C. G., Krüger, K., Besford, Q. A., Schlenk, M., Kempe, K., Förster, S., & Caruso, F. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.8b14307 · Cited by 66 · see citing works
High-Throughput Routes to Biomaterials Discovery
Soheilmoghaddam, M., Rumble, M., & Cooper-White, J. J. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01026 · Cited by 65 · see citing works
Alamán‐Díez, P., García‐Gareta, E., Arruebo, M., & Pérez, M. Á. (2022) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.37448 · Cited by 64 · see citing works
“Furthermore, working with microscale systems allows greater pH and temperature control and versatility that is not possible for macroscale cell studies.(10,11) Microfluidicbased devices are a good approach to evaluate the regulatory effects of single mechanical, chemical, and biochemical cues on cell behavior,”
BackgroundGuan, A., Hamilton, P., Wang, Y., Gorbet, M., Li, Z., & Phillips, K. S. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0045 · Cited by 63 · see citing works
Microfluidics for research and applications in oncology
Chaudhuri, P. K., Warkiani, M. E., Jing, T., Kenry, K., & Lim, C. T. (2015) · The Analyst · doi:10.1039/c5an00382b · Cited by 62 · see citing works
Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology
Pradhan, S., Banda, O. A., Farino, C. J., Sperduto, J. L., Keller, K. A., Taitano, R. A., & Slater, J. H. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901255 · Cited by 61 · see citing works
Mohammed, M. M., Thurgood, P., Gilliam, C., Nguyen, N., Pirogova, E., Peter, K., Khoshmanesh, K., & Baratchi, S. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b03247 · Cited by 56 · see citing works
Mechanoregulation of Vascular Endothelial Growth Factor Receptor 2 in Angiogenesis
Miller, B., & Sewell-Loftin, M. K. (2022) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.804934 · Cited by 56 · see citing works
Biomechanics of soft biological tissues and organs, mechanobiology, homeostasis and modelling
Holzapfel, G. A., Humphrey, J. D., & Ogden, R. W. (2025) · Journal of The Royal Society Interface · doi:10.1098/rsif.2024.0361 · Cited by 58 · see citing works
Shamloo, A., Mohammadaliha, N., & Mohseni, M. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.08.005 · Cited by 55 · see citing works
Koens, R., Tabata, Y., Serrano, J. C., Aratake, S., Yoshino, D., Kamm, R. D., & Funamoto, K. (2020) · APL Bioengineering · doi:10.1063/1.5127069 · Cited by 53 · see citing works
Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells
Trumbull, A., Subramanian, G., & Yildirim-Ayan, E. D. (2016) · BioMedical Engineering OnLine · doi:10.1186/s12938-016-0150-9 · Cited by 54 · see citing works
“These loading modalities are applied to stem cells and stem cellembedded tissue scaffolds using mechanical loading platforms, which were the focuses of excellent reviews in the literature [23–29].”
BackgroundMethodMicrofluidic modeling of the biophysical microenvironment in tumor cell invasion
Huang, Y., Segall, J. E., & Wu, M. (2017) · Lab on a Chip · doi:10.1039/c7lc00623c · Cited by 55 · see citing works
Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow
Calibasi Kocal, G., Güven, S., Foygel, K., Goldman, A., Chen, P., Sengupta, S., Paulmurugan, R., Baskin, Y., & Demirci, U. (2016) · Scientific Reports · doi:10.1038/srep38221 · Cited by 52 · see citing works
Doherty, E. L., Aw, W. Y., Hickey, A. J., & Polacheck, W. J. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.624435 · Cited by 53 · see citing works
Acoustic Cell Separation Based on Density and Mechanical Properties
Xie, Y., Mao, Z., Bachman, H., Li, P., Zhang, P., Ren, L., Wu, M., & Huang, T. J. (2020) · Journal of Biomechanical Engineering · doi:10.1115/1.4046180 · Cited by 52 · see citing works
Will microfluidics enable functionally integrated biohybrid robots?
Filippi, M., Yaşa, Ö., Kamm, R. D., Raman, R., & Katzschmann, R. K. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2200741119 · Cited by 51 · see citing works
Fibroblast Migration in 3D is Controlled by Haptotaxis in a Non-muscle Myosin II-Dependent Manner
Moreno-Arotzena, O., Borau, C., Movilla, N., Vicente‐Manzanares, M., & García‐Aznar, J. M. (2015) · Annals of Biomedical Engineering · doi:10.1007/s10439-015-1343-2 · Cited by 51 · see citing works
Local dynamic mechanical analysis for heterogeneous soft matter using ferrule-top indentation
van Hoorn, H., Kurniawan, N. A., Koenderink, G. H., & Iannuzzi, D. (2016) · Soft Matter · doi:10.1039/c6sm00300a · Cited by 49 · see citing works
Microfabrication and microfluidics for muscle tissue models
Uzel, S. G. M., Pavesi, A., & Kamm, R. D. (2014) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2014.08.013 · Cited by 50 · see citing works
“Although extensively reviewed in the past (see, e.g., (Polacheck et al. 2013)), this area of study is worth mentioning in the present context.”
BackgroundInfluentialModular soft robotic microdevices for dexterous biomanipulation
Özkale, B., Parreira, R., Bekdemir, A., Pancaldi, L., Özelçi, E., Amadio, C., Kaynak, M., Stellacci, F., Mooney, D. J., & Sakar, M. S. (2019) · Lab on a Chip · doi:10.1039/c8lc01200h · Cited by 48 · see citing works
Microfluidic technologies for vasculature biomimicry
Hu, C., Chen, Y., Tan, M. J. A., Ren, K., & Wu, H. (2019) · The Analyst · doi:10.1039/c9an00421a · Cited by 47 · see citing works
Modeling an Optimal 3D Skin-on-Chip within Microfluidic Devices for Pharmacological Studies
Fernández-Carro, E., Angenent, M. L., Gracia‐Cazaña, T., Gilaberte, Y., Alcaine, C., & Ciriza, J. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14071417 · Cited by 47 · see citing works
“Shear stress can be generated by passive delivery with a rocker, external pumping with a syringe pump or peristaltic pump, or internal pumping integrated within the chip (Figure 5) [78,81].”
BackgroundHypoxia‐enhanced adhesion of red blood cells in microscale flow
Kim, M., Alapan, Y., Adhikari, A., Little, J. A., & Gurkan, U. A. (2017) · Microcirculation · doi:10.1111/micc.12374 · Cited by 46 · see citing works
Arora, S., Lam, A. J. Y., Cheung, C., Yim, E. K. F., & Toh, Y. (2018) · Biotechnology and Bioengineering · doi:10.1002/bit.26910 · Cited by 46 · see citing works
Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues
Luciano, M., Versaevel, M., Vercruysse, E., Procès, A., Kalukula, Y., Remson, A., Deridoux, A., & Gabriele, S. (2022) · Biophysics Reviews · doi:10.1063/5.0074317 · Cited by 44 · see citing works
Biophysical Tools to Study Cellular Mechanotransduction
Muhamed, I., Chowdhury, F., & Maruthamuthu, V. (2017) · Bioengineering · doi:10.3390/bioengineering4010012 · Cited by 43 · see citing works
“developed a multi-compartment shear flow microfluidic platform using a braille piezoelectric array of pins that functions as valves with a multi-step peristaltic pumping sequence [149,154].”
MethodCell migration in microengineered tumor environments
Um, E., Oh, J. M., Granick, S., & Cho, Y. (2017) · Lab on a Chip · doi:10.1039/c7lc00555e · Cited by 44 · see citing works
Luminal flow actuation generates coupled shear and strain in a microvessel-on-chip
Dessalles, C. A., Ramón-Lozano, C., Babataheri, A., & Barakat, A. I. (2021) · Biofabrication · doi:10.1088/1758-5090/ac2baa · Cited by 43 · see citing works
Flow-Induced Shear Stress Primes NLRP3 Inflammasome Activation in Macrophages via Piezo1
Fish, A., & Kulkarni, A. A. (2024) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.3c18645 · Cited by 42 · see citing works
Kucińska, M., Murias, M., & Nowak-Sliwinska, P. M. (2016) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2016.09.002 · Cited by 41 · see citing works
Weiss, A. C. G., Kempe, K., Förster, S., & Caruso, F. (2018) · Biomacromolecules · doi:10.1021/acs.biomac.8b00196 · Cited by 41 · see citing works
Engineering Biomaterials and Approaches for Mechanical Stretching of Cells in Three Dimensions
Zhang, W., Huang, G., & Xu, F. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.589590 · Cited by 42 · see citing works
Mechanobiology of the female reproductive system
Matsuzaki, S. (2021) · Reproductive Medicine and Biology · doi:10.1002/rmb2.12404 · Cited by 37 · see citing works
Organ-on-a-Chip Approach for Accelerating Blood–Brain Barrier Nanoshuttle Discovery
Choi, J.-W., Kim, K., Mukhambetiyar, K., Lee, N. K., del Río, J. S., Joo, J., Park, C. G., Kwon, T., & Park, T. (2024) · ACS Nano · doi:10.1021/acsnano.4c00994 · Cited by 37 · see citing works
Greineder, C. F., Johnston, I. H., Villa, C. H., Gollomp, K. L., Esmon, C. T., Cines, D. B., Poncz, M., & Muzykantov, V. R. (2017) · Blood Advances · doi:10.1182/bloodadvances.2017007229 · Cited by 37 · see citing works
“First, PDMS flow chambers are rectangular in cross-section and fail to reflect the mechanical compliance of normal vasculature.(56) Use of linear channels with fixed dimensions and no branches limits extrapolation to physiologic vascular beds, where inflammatory, TF-driven thrombosis may occur heterogeneously.”
BackgroundOrganoids meet microfluidics: recent advancements, challenges, and future of organoids-on-chip
Chauhdari, T., Zaidi, S. A., Su, J., & Ding, Y. (2025) · In vitro models · doi:10.1007/s44164-025-00086-7 · Cited by 41 · see citing works
“Lev-eraging microfabrication technology, these devices feature micropatterned channels and reservoirs with external air or liquid pumps attached that enable precise control over the biochemical and mechanical environment [69, 70].”
The role of mechanics in actin stress fiber kinetics
Elson, E. L., & Genin, G. M. (2013) · Experimental Cell Research · doi:10.1016/j.yexcr.2013.06.017 · Cited by 35 · see citing works
“, [9-16]), these observations suffice as a backdrop for the foregoing discussion.”
BackgroundMechanically activated artificial cell by using microfluidics
Ho, K. K. Y., Lee, L. M., & Liu, A. P. (2016) · Scientific Reports · doi:10.1038/srep32912 · Cited by 36 · see citing works
Microfluidics for the study of mechanotransduction
Griffith, C. M., Huang, S. A., Cho, C., Khare, T. M., Rich, M. J., Lee, G.-H., Ligler, F. S., Diekman, B. O., & Polacheck, W. J. (2020) · Journal of Physics D Applied Physics · doi:10.1088/1361-6463/ab78d4 · Cited by 34 · see citing works
“Ductal epithelial cells are subjected to fluid shear stress from flowing glandular secretions and blood filtrate, and microfluidic platforms similar to those developed for the vasculature have been fabricated to investigate the molecular mechanisms of mechanotransduction in many type of epithelial cells (reviewed [152…”
BackgroundOrgan-on-a-Chip and Microfluidic Platforms for Oncology in the UK
Nolan, J., Pearce, O. M. T., Screen, H. R. C., Knight, M. M., & Verbruggen, S. W. (2023) · Cancers · doi:10.3390/cancers15030635 · Cited by 35 · see citing works
“The field of microfluidics presented itself as an ideal way for bioengineers to manipulate cell mechanobiology [27], whereas the innovations involved in organ chip design have produced novel approaches to building organotypic mechanical signals in vitro [28].”
BackgroundSingle-cell trapping and selective treatment via co-flow within a microfluidic platform
Benavente‐Babace, A., Gallego‐Perez, D., Hansford, D. J., Arana, S., Pérez-Lorenzo, E., & Mujika, M. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.05.036 · Cited by 35 · see citing works
Advancing Intelligent Organ‐on‐a‐Chip Systems with Comprehensive In Situ Bioanalysis
Li, X., Zhu, H. J., Gu, B., Yao, C., Gu, Y., Xu, W., Zhang, J., He, J., Liu, X., & Li, D. (2023) · Advanced Materials · doi:10.1002/adma.202305268 · Cited by 37 · see citing works
A microfluidic device for characterizing nuclear deformations
Hodgson, A. C., Verstreken, C. M., Fisher, C. L., Keyser, U. F., Pagliara, S., & Chalut, K. J. (2017) · Lab on a Chip · doi:10.1039/c6lc01308b · Cited by 34 · see citing works
Microfluidic approaches for probing amyloid assembly and behaviour
Herling, T. W., Levin, A., Saar, K. L., Dobson, C. M., & Knowles, T. P. J. (2018) · Lab on a Chip · doi:10.1039/c7lc01241a · Cited by 33 · see citing works
Hesari, Z., Soleimani, M., Atyabi, F., Sharifdini, M., Nadri, S., Warkiani, M. E., Zare, M., & Dinarvand, R. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35689 · Cited by 34 · see citing works
“With the advent of microfluidics, many of the previous hurdles of in vitro testing were eliminated through greater control or combined functionalities, allowing one to create specific micro-environment with in vivo-like physiological topography.(27,28) In this work, we utilized the power of micro-engineering to develo…”
MethodMechanobiology of the endothelium in vascular health and disease: in vitro shear stress models
Jackson, M. L., Bond, A., & George, S. J. (2022) · Cardiovascular Drugs and Therapy · doi:10.1007/s10557-022-07385-1 · Cited by 33 · see citing works
In situ biosensing technologies for an organ-on-a-chip
Kim, J., Kim, J., Jin, Y., & Cho, S. (2023) · Biofabrication · doi:10.1088/1758-5090/aceaae · Cited by 34 · see citing works
Blood brain barrier: A tissue engineered microfluidic chip
Musafargani, S., Mishra, S., Gulyás, M., Mahalakshmi, P., Archunan, G., Padmanabhan, P., & Gulyás, B. Z. (2019) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2019.108525 · Cited by 32 · see citing works
Microfluidic Platforms to Screen Granular Hydrogel Microenvironments for Tissue Regeneration
Krattiger, L. A., Emiroglu, D. B., Pravato, S., Moser, L. O., Bachmann, O. A., Cioppa, S. Y. L., Rodriguez‐Rivera, G. J., Burdick, J. A., deMello, A. J., Tibbitt, M. W., et al. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202310507 · Cited by 31 · see citing works
Hot or cold: Bioengineering immune contextures into in vitro patient-derived tumor models
Too, N. S. H., Ho, N. C. W., Adine, C., Iyer, N. G., & Fong, E. L. S. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.05.001 · Cited by 31 · see citing works
Moreno-Arotzena, O., Mendoza, G., Cóndor, M., Rüberg, T., & García‐Aznar, J. M. (2014) · Biomicrofluidics · doi:10.1063/1.4903948 · Cited by 30 · see citing works
Universal and One-Step Modification to Render Diverse Materials Bioactivation
Chen, Q., Zhang, X., Zhang, D., Liu, G., Ma, K., Liu, J., Ma, K., Chen, M., Li, Y., & Liu, R. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.3c05928 · Cited by 30 · see citing works
Controlled Rotation and Vibration of Patterned Cell Clusters Using Dielectrophoresis
Soffe, R., Tang, S., Baratchi, S., Nahavandi, S., Nasabi, M., Cooper, J. M., Mitchell, A., & Khoshmanesh, K. (2015) · Analytical Chemistry · doi:10.1021/ac5043335 · Cited by 30 · see citing works
A Flexible Microdevice for Mechanical Cell Stimulation and Compression in Microfluidic Settings
Onal, S., Alkaisi, M. M., & Nock, V. (2021) · Frontiers in Physics · doi:10.3389/fphy.2021.654918 · Cited by 29 · see citing works
“Cancer cells alter their own biophysical properties and exert physical forces during primary tumor growth and then to spread, invade, or metastasize [6, 7].”
BackgroundSundaram, P. M., Rangharajan, K. K., Akbari, E., Hadick, T., Song, J. W., & Prakash, S. (2020) · Lab on a Chip · doi:10.1039/d0lc00507j · Cited by 29 · see citing works
Caring for cells in microsystems: principles and practices of cell-safe device design and operation
Varma, S., & Joel Voldman, B. (2018) · Lab on a Chip · doi:10.1039/c8lc00746b · Cited by 30 · see citing works
Poon, C. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.08.25.266221 · Cited by 29 · see citing works
“In culture devices that involve flow such as bioreactors [2-6] and microfluidic organ-on-chips [7-10], culture fluid is the primary material through which mechanical loading is exerted on cells.”
Background
- Authors
- Polacheck, W. J., Li, R., Uzel, S. G. M., & Kamm, R. D.
- Affiliations
- Massachusetts Institute of Technology (US)
- Corresponding author
- Kamm, Roger D.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2013
- Volume
- 13
- Issue
- 12
- Pages
- 2252
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC3714214
- OpenAlex
- W2144850941
- Cited by
- 272
- References
- 133
- Citations by year
- 2026: 15 · 2025: 19 · 2024: 14 · 2023: 22 · 2022: 16 · 2021: 22 · 2020: 20 · 2019: 18 · 2018: 22 · 2017: 29 · 2016: 34 · 2015: 19 · 2014: 16 · 2013: 5
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Lab on a Chip (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- National Science Foundation (0939511)
- Emergent Behaviors of Integrated Cellular Systems (0939511, CBET0939511))
- National Cancer Institute (R21CA140096)
- Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET-0939511, 0939511)
- Janssen Pharmaceuticals
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- mechanotransduction, mechanobiology, microfluidic platform, mechanical forces, cell imaging
- MeSH terms
- Blood Cells, Epithelial Cells, Humans, Neoplastic Cells, Circulating, Muscle Cells, Shear Strength, Mechanotransduction, Cellular, Microfluidic Analytical Techniques
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