Carotenoids exclusively synthesized in red pepper (capsanthin and capsorubin) protect human dermal fibroblasts against UVB induced DNA damage
Open accessAbstract
Photoprotection by dietary carotenoids has been linked to their antioxidant properties, in particular quenching of singlet molecular oxygen and scavenging of peroxyl radicals. Here, we compared the DNA-protection and antioxidant effects of selected carotenoids exclusively synthesized in red pepper (capsanthin and capsorubin) to the xanthophyll lutein. Preincubation of human dermal fibroblasts (hdf) with capsanthin and capsorubin significantly counteracted UVB induced cytotoxicity at doses between 0 and 300 mJ cm(-2). Pretreatment of hdf with capsanthin, capsorubin or lutein (1 μM) significantly decreased the formation of DNA strand breaks following irradiation with UVB light. All carotenoids studied decreased caspase-3 cleavage (a marker for UVB-induced apoptosis), however, caspase dependent PARP-1 cleavage was not affected suggesting that the remaining caspase activity is sufficient to promote UVB-induced apoptosis. It is conceivable that carotenoids selectively interfere with cellular responses activated by UVB-mediated damage. Our findings indicate that capsanthin and capsorubin exhibit similar properties to lutein and could be used as a dietary supplement to improve natural photoprotection.
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Cell pretreatment with capsanthin and capsorubin decreased UVB-induced DNA strand breaks and counteracted caspase-3 activation.
“Indeed, cell pretreatment with these carotenoids decreased the formation of UVB-induced DNA strand breaks and counteracted caspase-3 activation [108].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [108]
Capsanthin and capsorubin from red pepper fruits have protective properties against UVB-induced DNA damage in human dermal fibroblasts.
“Carotenoids capsanthin and capsorubin from red pepper (Capsicum annuum L.) fruits have also been found to have protective properties against UVB-induced DNA damage in human dermal fibroblasts [88, 108].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [108]
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- Authors
- Fernández-García, E., Carvajal-Lérida, I., & Pérez-Gálvez, A.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Venue
- Photochemical & Photobiological Sciences
- Published
- 2016-09
- Volume
- 15
- Issue
- 9
- Pages
- 1204-1211
- ISSN
- 1474-905X (ISSN-L)
- 1474-9092
- OpenAlex
- W2518430325
- Cited by
- 40
- References
- 31
- Citations by year
- 2026: 8 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 2
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Photochemical & Photobiological Sciences (publishedVersion)
- PubMed (publishedVersion)
- RISalud Institutional Health Repository of Andalucía (submittedVersion, other-oa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- capsanthin, capsorubin, lutein, carotenoids, UVB-induced DNA damage, photoprotection, human dermal fibroblasts, DNA strand breaks, apoptosis, caspase-3, antioxidant activity, dietary carotenoids, red pepper
- MeSH terms
- Antioxidants, Capsicum, Cell Survival, Cells, Cultured, DNA Damage, Dose-Response Relationship, Drug, Fibroblasts, Humans, Skin, Structure-Activity Relationship, Ultraviolet Rays, Lutein, Molecular Structure, Apoptosis, Xanthophylls
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