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The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa

Bondí, Roslen, Longo, Francesca, Messina, Marco, D'Angelo, Francesca, Visca, Paolo, Leoni, Livia, Rampioni, Giordano·2017·Molecular BioSystems
DOI 10.1039/c7mb00040e
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Abstract

Quorum sensing (QS) is an intercellular communication system which controls virulence-related phenotypes in the human pathogen Pseudomonas aeruginosa. LasR is the QS receptor protein which responds to the signal molecule N-(3-oxododecanoyl)homoserine lactone (3OC12-HSL) and promotes signal production by increasing the transcription of the 3OC12-HSL synthase gene, lasI. LasR also activates the expression of other genes, including rsaL, coding for the RsaL protein which acts as a transcriptional repressor of lasI. Direct gene activation and RsaL-mediated gene repression, both exerted by LasR on the expression of the output gene lasI, generate a regulatory network motif known as the type 1 incoherent feedforward loop (IFFL-1) that governs 3OC12-HSL production. In addition to lasI, RsaL directly represses a set of LasR-activated genes; hence, the IFFL-1 generated by LasR and RsaL is a multi-output IFFL-1. Here we demonstrate that the multi-output IFFL-1 constituted by LasR and RsaL confers robustness with respect to fluctuations in the levels of LasR to the phenotypes controlled by both these transcriptional regulators (e.g. 3OC12-HSL synthesis and pyocyanin production). In contrast, other virulence-related phenotypes controlled by LasR but not by RsaL (e.g. elastase and protease production) are sensitive to changes in LasR levels. Overall, the multi-output IFFL-1 generated by LasR and RsaL splits the QS regulon into two distinct sub-regulons with different robustness with respect to LasR fluctuations. This emerging regulatory property enhances the phenotypic plasticity of P. aeruginosa, thus contributing to its adaptation to changing environments.

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Metadata

Authors
Bondí, R., Longo, F., Messina, M., D'Angelo, F., Visca, P., Leoni, L., & Rampioni, G.
Venue
Molecular BioSystems
Publisher
Oxford University Press (OUP)
Published
2017-04-20
Volume
13
Issue
6
Pages
1080-1089
ISSN
  • 1742-2051
  • 1742-206X
DOI
10.1039/c7mb00040e
Cited by
21
References
3
Language
en
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)

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CoChat. (2026). Citation check: The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa. https://app.cochat.ai/papers/10.1039/c7mb00040e
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Bondí, R., Longo, F., Messina, M., D'Angelo, F., Visca, P., Leoni, L., & Rampioni, G. (2017). The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa. Molecular BioSystems. https://doi.org/10.1039/c7mb00040e

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