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The basal proton conductance of mitochondria depends on adenine nucleotide translocase content
Open accessAbstract
The basal proton conductance of mitochondria causes mild uncoupling and may be an important contributor to metabolic rate. The molecular nature of the proton-conductance pathway is unknown. We show that the proton conductance of muscle mitochondria from mice in which isoform 1 of the adenine nucleotide translocase has been ablated is half that of wild-type controls. Overexpression of the adenine nucleotide translocase encoded by the stress-sensitive B gene in Drosophila mitochondria increases proton conductance, and underexpression decreases it, even when the carrier is fully inhibited using carboxyatractylate. We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions.
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“Underlining their importance, ANTs can account for up to 50% of basal mitochondrial membrane proton conductance [25].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [25]
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Metadata
- Authors
- Brand, M. D., Pakay, J. L., Ocloo, A., Kokoszka, J., Wallace, D. C., Brookes, P. S., & Cornwall, E. J.
- Affiliations
- MRC Mitochondrial Biology Unit (GB)
- Emory University (US)
- Department of Molecular and Cellular Pathology, University of Birmingham, Birmingham, AL 35294, U.S.A.
- Corresponding author
- Ocloo, Augustine
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2005-11-22
- Volume
- 392
- Issue
- 2
- Pages
- 353-362
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1316271
- OpenAlex
- W1996324277
- Cited by
- 410
- References
- 52
- Citations by year
- 2026: 6 · 2025: 20 · 2024: 13 · 2023: 23 · 2022: 25 · 2021: 20 · 2020: 25 · 2019: 17 · 2018: 13 · 2017: 19 · 2016: 14 · 2015: 18 · 2014: 23 · 2013: 21 · 2012: 23
- FWCI
- 6.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cambridge Commonwealth Trust
- National Institutes of Health
- Medical Research Council
- National Institute of Neurological Disorders and Stroke (NS 41850, R01NS041850)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- adenine nucleotide translocase, adenine nucleotide carrier, mitochondrial uncoupling, muscle mitochondria, B gene, carboxyatractylate, ADP/ATP exchange, metabolic rate
- MeSH terms
- Mitochondrial ADP, ATP Translocases, Animals, Cattle, Drosophila melanogaster, Female, Horses, Male, Membrane Potentials, Mitochondria, Phospholipids, Protein Binding, Protons, Rabbits, Swine, Gene Expression Regulation, Enzymologic, Mice, Knockout, Cell Respiration, Drosophila Proteins, Mice, Rats
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