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Cited by 210Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressCardiovascular Health and Disease Prevention

Chemical nature of nitric oxide storage forms in rat vascular tissue

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Rodriguez, Juan, Maloney, Ronald E., Rassaf, Tienush, Bryan, Nathan S., Feelisch, Martin·2003·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0234600100PMC PMC140970
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Abstract

Endothelial NO production results in local formation of adducts that may act as storage forms of NO. Because little is known about their chemical nature, concentrations, and possible role in vascular biology, we sought to characterize those species basally present in rat aorta, using two independent approaches. In the first approach, tissue homogenates were analyzed by using chemiluminescence- and ion-chromatography-based techniques that allow trace-level quantification of NO-related compounds in complex biological matrices. In the second approach, NO stores were characterized by their ability to release NO when illuminated with light and subsequently relax vascular smooth muscle (photorelaxation). The latter included a careful assessment of action spectra for photorelaxation, taking into account the light-scattering properties of the tissue and the storage depletion rates induced by exposure to controlled levels of light. Biochemical analyses revealed that aortic tissues contained 10 +/- 1 microM nitrite, 42 +/- 7 microM nitrate, 40 +/- 6 nM S-nitroso, and 33 +/- 6 nM N-nitroso compounds (n = 4-8). The functional data obtained suggest that the NO photolytically released in the tissue originated from species with photophysical properties similar to those reported for low-molecular-weight S-nitrosothiols, as well as from nitrite. The relative contribution of these potential NO stores to the extent of photorelaxation was consistent with their concentrations detected biochemically in vascular tissue when their photoactivity was taken into account. We conclude that intravascular nitroso species and nitrite both have the potential to release physiologically relevant quantities of NO independent of enzymatic control by NO synthase.

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  • NO is readily oxidized to nitrite and subsequently nitrate in an oxygen dependent manner; physiological concentrations of nitrite vary between tissues reflecting the degree of NOS activity as well as oxidation pathways of NO.

    “NO is readily oxidized to nitrite and subsequently nitrate in an oxygen dependent manner; thus, the physiological concentrations of nitrite vary between tissues reflecting the degree of NOS activity as well as oxidation pathways of NO [20, 45].”

    From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [45]

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  1. [4]Furchgott R F, Sleaton W, Jr, McCaman M W, Elchleff J. J Pharmacol Exp Ther. 1955;113:22–23. [Google Scholar]
  2. [7]Karlsson J O, Axelsson K L, Elwing H, Andersson R G. Cyclic Nucleotide Protein Phosphor Res. 1986;11:155–166. [PubMed] [Google Scholar]
  3. [10]Büyükafsar K, Demirel-Yilmaz E, Göçmen C, Dikmen A. J Pharmacol Exp Ther. 1999;290:768–773. [PubMed] [Google Scholar]
  4. [11]Trivedi H D, Kelkar V V, Jindal M N, Dave K C. Ind J Physiol Pharmacol. 1978;22:136–141. [PubMed] [Google Scholar]
  5. [14]Matsunaga K, Furchgott R F. J Pharmacol Exp Ther. 1991;259:1140–1146. [PubMed] [Google Scholar]
  6. [15]Jankowsky J J, Keiber D J, Mopper K. Photochem Photobiol. 1999;70:319–328. [Google Scholar]
  7. [18]Venturini C M, Palmer R M, Moncada S. J Pharmacol Exp Ther. 1993;266:1497–1500. [PubMed] [Google Scholar]
  8. [19]Wood P D, Mutus B, Redmond R W. Photochem Photobiol. 1996;64:518–524. [Google Scholar]
  9. [20]Zhang Y Y, Xu A M, Nomen M, Walsh M, Keaney J F, Jr, Loscalzo J. J Biol Chem. 1996;271:14271–14279. [PubMed] [Google Scholar]
  10. [23]Bourassa J, DeGraff W, Kudo S, Wink D A, Mitchell J B, Ford P C. J Am Chem Soc. 1997;119:2853–2860. [Google Scholar]
  11. [24]Lobysheva I I, Serezhenkov V A, Vanin A F. Biochemistry (Moscow) 1999;64:153–158. [PubMed] [Google Scholar]
  12. [30]Rohlfs R J, Olson J S, Gibson Q H. J Biol Chem. 1988;263:1803–1813. [PubMed] [Google Scholar]
  13. [31]Matsunaga K, Furchgott R F. J Pharmacol Exp Ther. 1988;248:687–695. [PubMed] [Google Scholar]

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Authors
Rodriguez, J., Maloney, R. E., Rassaf, T., Bryan, N. S., & Feelisch, M.
Affiliations
Louisiana State University Health Sciences Center Shreveport (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2002-12-26
Volume
100
Issue
1
Pages
336-341
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0234600100
PMC
PMC140970
OpenAlex
W2068642993
Cited by
210
References
36
Citations by year
2025: 4 · 2024: 3 · 2023: 4 · 2022: 8 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 15 · 2013: 2 · 2012: 8
FWCI
10.21
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • ePrints Soton (University of Southampton) (submittedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • National Institutes of Health (HL69029-01)
  • National Heart, Lung, and Blood Institute (HL69029-01, R01HL069029)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
Keywords
nitric oxide storage, nitric oxide adducts, S-nitrosothiols, N-nitroso compounds, nitrite, nitrate, photorelaxation, vasorelaxation, aorta, endothelial nitric oxide, action spectrum
MeSH terms
Animals, Aorta, Thoracic, Light, Male, Muscle, Smooth, Vascular, Nitrates, Nitric Oxide, Nitrites, Nitroso Compounds, Photochemistry, Photolysis, Scattering, Radiation, Rats, Wistar, Nitric Oxide Synthase, Rats

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