Pyramidal neurons of upper cortical layers generated by NEX-positive progenitor cells in the subventricular zone
Open accessAbstract
The generation of pyramidal neurons in the mammalian neocortex has been attributed to proliferating progenitor cells within the ventricular zone (VZ). Recently, the subventricular zone (SVZ) has been recognized as a possible source of migratory neurons in brain slice preparations, but the relevance of these observations for the developing neocortex in vivo remains to be defined. Here, we demonstrate that a subset of progenitor cells within the SVZ of the mouse neocortex can be molecularly defined by Cre recombinase expression under control of the NEX/Math2 locus, a neuronal basic helix-loop-helix gene that by itself is dispensable for cortical development. NEX-positive progenitors are generated by VZ cells, move into the SVZ, and undergo multiple asymmetrical and symmetrical cell divisions that produce a fraction of the neurons in the upper cortical layers. Our data suggest that NEX-positive progenitors within the SVZ are committed to a glutamatergic neuronal fate and have evolved to expand the number of cortical output neurons that is characteristic for the mammalian forebrain.
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IPCs can undergo 1-3 proliferation cycles before differentiation to neurons.
“IPCs can undergo 1–3 proliferation cycles before differentiation to neurons [97, 98].”
From The ASD Living Biology: from cell proliferation to clinical phenotype (2019) · cites this paper as [98]
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- Authors
- Wu, S.-X., Goebbels, S., Nakamura, K., Nakamura, K., Kometani, K., Minato, N., Kaneko, T., Nave, K.-A., & Tamamaki, N.
- Affiliations
- Department of Morphological Brain Science and Laboratory of Immunological Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Anatomy and K. K. Leung Brain Research Centre, Fourth Military Medical University, Xi'an 710032, People's Republic of China; Department of Neurogenetics, Max Planck Institute of Experimental Medicine, D-37075 Goettingen, Germany; Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology,...
- Kyoto University (JP)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2005-11-11
- Volume
- 102
- Issue
- 47
- Pages
- 17172-17177
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1288007
- OpenAlex
- W2024238415
- Cited by
- 198
- References
- 31
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 9 · 2022: 3 · 2021: 16 · 2020: 8 · 2019: 9 · 2018: 9 · 2017: 7 · 2016: 13 · 2015: 6 · 2014: 11 · 2013: 12 · 2012: 14
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- pyramidal neurons, subventricular zone, cortical progenitor cells, neocortex development, glutamatergic neurons, neuronal fate specification, cell migration, asymmetric cell division, ventricular zone, basic helix-loop-helix genes
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Cerebral Ventricles, Mice, Inbred C57BL, Mice, Transgenic, Nerve Tissue Proteins, Stem Cells, Genes, Reporter, Pyramidal Cells, Cell Lineage, Neocortex, Mice, Basic Helix-Loop-Helix Proteins
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