Cited by 198Neurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene RegulationCongenital heart defects research

Pyramidal neurons of upper cortical layers generated by NEX-positive progenitor cells in the subventricular zone

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Wu, Sheng-Xi, Goebbels, Sandra, Nakamura, Kouichi, Nakamura, Kazuhiro, Kometani, Kouhei, Minato, Nagahiro, Kaneko, Takeshi, Nave, Klaus-Armin +1 more·2005·Proceedings of the National Academy of Sciences

Abstract

The generation of pyramidal neurons in the mammalian neocortex has been attributed to proliferating progenitor cells within the ventricular zone (VZ). Recently, the subventricular zone (SVZ) has been recognized as a possible source of migratory neurons in brain slice preparations, but the relevance of these observations for the developing neocortex in vivo remains to be defined. Here, we demonstrate that a subset of progenitor cells within the SVZ of the mouse neocortex can be molecularly defined by Cre recombinase expression under control of the NEX/Math2 locus, a neuronal basic helix-loop-helix gene that by itself is dispensable for cortical development. NEX-positive progenitors are generated by VZ cells, move into the SVZ, and undergo multiple asymmetrical and symmetrical cell divisions that produce a fraction of the neurons in the upper cortical layers. Our data suggest that NEX-positive progenitors within the SVZ are committed to a glutamatergic neuronal fate and have evolved to expand the number of cortical output neurons that is characteristic for the mammalian forebrain.

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Crossref · 10.1073/pnas.0508560102

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Authors
Wu, S.-X., Goebbels, S., Nakamura, K., Nakamura, K., Kometani, K., Minato, N., Kaneko, T., Nave, K.-A., & Tamamaki, N.
Affiliations
  • Department of Morphological Brain Science and Laboratory of Immunological Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Anatomy and K. K. Leung Brain Research Centre, Fourth Military Medical University, Xi'an 710032, People's Republic of China; Department of Neurogenetics, Max Planck Institute of Experimental Medicine, D-37075 Goettingen, Germany; Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology,...
  • Kyoto University (JP)
Venue
Proceedings of the National Academy of Sciences
Published
2005-11-11
Volume
102
Issue
47
Pages
17172-17177
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2024238415
Cited by
198
References
31
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 9 · 2022: 3 · 2021: 16 · 2020: 8 · 2019: 9 · 2018: 9 · 2017: 7 · 2016: 13 · 2015: 6 · 2014: 11 · 2013: 12 · 2012: 14
FWCI
3.60
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
Keywords
pyramidal neurons, subventricular zone, cortical progenitor cells, neocortex development, glutamatergic neurons, neuronal fate specification, cell migration, asymmetric cell division, ventricular zone, basic helix-loop-helix genes
MeSH terms
Animals, Cell Differentiation, Cells, Cultured, Cerebral Ventricles, Mice, Inbred C57BL, Mice, Transgenic, Nerve Tissue Proteins, Stem Cells, Genes, Reporter, Pyramidal Cells, Cell Lineage, Neocortex, Mice, Basic Helix-Loop-Helix Proteins

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