Cited by 253Lipid metabolism and biosynthesisLipid metabolism and disordersCancer, Lipids, and Metabolism

Evolutionarily conserved gene family important for fat storage

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Kadereit, Bert, Kumar, Pradeep, Wang, Wen-Jun, Miranda, Diego, Snapp, Erik L., Severina, Nadia, Torregroza, Ingrid, Evans, Todd +1 more·2008·Proceedings of the National Academy of Sciences

Abstract

The ability to store fat in the form of cytoplasmic triglyceride droplets is conserved from Saccharomyces cerevisiae to humans. Although much is known regarding the composition and catabolism of lipid droplets, the molecular components necessary for the biogenesis of lipid droplets have remained obscure. Here we report the characterization of a conserved gene family important for lipid droplet formation named fat-inducing transcript (FIT). FIT1 and FIT2 are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation in cell culture and when expressed in mouse liver. shRNA silencing of FIT2 in 3T3-LI adipocytes prevents accumulation of lipid droplets, and depletion of FIT2 in zebrafish blocks diet-induced accumulation of lipid droplets in the intestine and liver, highlighting an important role for FIT2 in lipid droplet formation in vivo. Together these studies identify and characterize a conserved gene family that is important in the fundamental process of storing fat.

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  1. [7]Mullner H, Daum G. Acta Biochim Pol. 2004;51:323–347. [PubMed] [Google Scholar]
  2. [29]Castelein H, Gulick T, Declercq PE, Mannaerts GP, Moore DD, Baes MI. J Biol Chem. 1994;269:26754–26758. [PubMed] [Google Scholar]

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Authors
Kadereit, B., Kumar, P., Wang, W.-J., Miranda, D., Snapp, E. L., Severina, N., Torregroza, I., Evans, T., & Silver, D. L.
Affiliations
  • *Department of Biochemistry and the Marion Bessin Liver Research Center and
  • Albert Einstein College of Medicine (US)
  • University of California, San Francisco (US)
Corresponding author
Silver, David L.
Venue
Proceedings of the National Academy of Sciences
Published
2008-01-08
Volume
105
Issue
1
Pages
94-99
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2163138997
Cited by
252
References
37
Citations by year
2026: 12 · 2025: 7 · 2024: 9 · 2023: 13 · 2022: 23 · 2021: 15 · 2020: 15 · 2019: 17 · 2018: 22 · 2017: 15 · 2016: 10 · 2015: 13 · 2014: 12 · 2013: 9 · 2012: 12
FWCI
4.18
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (DK41296)
  • National Institute on Aging (R21AG032544)
  • National Heart, Lung, and Blood Institute (R01HL064282)
  • National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK41296, P30DK041296)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
lipid droplet formation, fat deposition, endoplasmic reticulum membrane protein, adipocytes, zebrafish, mouse liver
MeSH terms
Adipose Tissue, Amino Acid Sequence, Animals, Diabetes Mellitus, Experimental, Gene Expression Regulation, Humans, Lipids, Liver, Membrane Proteins, Models, Biological, Molecular Sequence Data, Triglycerides, Zebrafish, Conserved Sequence, Sequence Homology, Amino Acid, Adipocytes, Evolution, Molecular, 3T3-L1 Cells, Mice

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