Stabilization of HIF-1α is critical to improve wound healing in diabetic mice
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Relative hypoxia is essential in wound healing since it normally plays a pivotal role in regulation of all the critical processes involved in tissue repair. Hypoxia-inducible factor (HIF) 1α is the critical transcription factor that regulates adaptive responses to hypoxia. HIF-1α stability and function is regulated by oxygen-dependent soluble hydroxylases targeting critical proline and asparaginyl residues. Here we show that hyperglycemia complexly affects both HIF-1α stability and activation, resulting in suppression of expression of HIF-1 target genes essential for wound healing both in vitro and in vivo . However, by blocking HIF-1α hydroxylation through chemical inhibition, it is possible to reverse this negative effect of hyperglycemia and to improve the wound healing process (i.e., granulation, vascularization, epidermal regeneration, and recruitment of endothelial precursors). Local adenovirus-mediated transfer of two stable HIF constructs demonstrated that stabilization of HIF-1α is necessary and sufficient for promoting wound healing in a diabetic environment. Our findings outline the necessity to develop specific hydroxylase inhibitors as therapeutic agents for chronic diabetes wounds. In conclusion, we demonstrate that impaired regulation of HIF-1α is essential for the development of diabetic wounds, and we provide evidence that stabilization of HIF-1α is critical to reverse the pathological process.
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Hypoxia and angiogenesis are causally linked to wound repair, which collectively induces substantial molecular and morphological changes to tissues during repair.
“hypoxia and angiogenesis are causally linked to wound repair, which collectively induces substantial molecular and morphological changes to tissues during repair.78,79”
From Single-cell transcriptomics of human-skin-equivalent organoids (2023) · cites this paper as [78]
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- Authors
- Botusan, I. R., Sunkari, V. G., Savu, O., Catrina, A. I., Grünler, J., Lindberg, S., Pereira, T., Ylä-Herttuala, S., Poellinger, L., Brismar, K., & Catrina, S.-B.
- Affiliations
- Carol Davila University of Medicine and Pharmacy (RO)
- Karolinska Institutet (SE)
- Karolinska University Hospital (SE)
- Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Finland
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-12-09
- Volume
- 105
- Issue
- 49
- Pages
- 19426-19431
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2614777
- OpenAlex
- W2163809998
- Cited by
- 533
- References
- 39
- Citations by year
- 2026: 38 · 2025: 36 · 2024: 35 · 2023: 33 · 2022: 35 · 2021: 38 · 2020: 37 · 2019: 36 · 2018: 30 · 2017: 31 · 2016: 29 · 2015: 27 · 2014: 31 · 2013: 30 · 2012: 21
- FWCI
- 16.10
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Åke Wiberg Stiftelse
- Vetenskapsrådet
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
- Keywords
- HIF-1α, hypoxia-inducible factor, wound healing, diabetic wounds, hyperglycemia, protein stabilization, chronic wounds, granulation tissue, vascularization, epidermal regeneration, endothelial precursors, adenoviral gene transfer, diabetic mice
- MeSH terms
- Amino Acids, Dicarboxylic, Animals, Hypoxia, Chronic Disease, Disease Models, Animal, Enzyme Inhibitors, Fibroblasts, Humans, Mixed Function Oxygenases, Hyperglycemia, Kidney Neoplasms, Mice, Inbred C57BL, Mice, Mutant Strains, Wound Healing, Gene Expression, 3T3 Cells, Diabetic Foot, Dermis, Cell Line, Tumor, Mice, Hypoxia-Inducible Factor 1, alpha Subunit
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