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Cited by 310

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

MicroRNA miR-125a controls hematopoietic stem cell number

Guo, Shangqin, Lu, Jun, Schlanger, Rita, Zhang, Hao, Wang, Judy Y., Fox, Michelle C., Purton, Louise E., Fleming, Heather H., Cobb, Bradley, Merkenschlager, Matthias, Golub, Todd R., Scadden, David T.·2010·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0913574107
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Abstract

MicroRNAs influence hematopoietic differentiation, but little is known about their effects on the stem cell state. Here, we report that the microRNA processing enzyme Dicer is essential for stem cell persistence in vivo and a specific microRNA, miR-125a, controls the size of the stem cell population by regulating hematopoietic stem/progenitor cell (HSPC) apoptosis. Conditional deletion of Dicer revealed an absolute dependence for the multipotent HSPC population in a cell-autonomous manner, with increased HSPC apoptosis in mutant animals. An evolutionarily conserved microRNA cluster containing miR-99b, let-7e, and miR-125a was preferentially expressed in long-term hematopoietic stem cells. MicroRNA miR-125a alone was capable of increasing the number of hematopoietic stem cells in vivo by more than 8-fold. This result was accomplished through a differentiation stage-specific reduction of apoptosis in immature hematopoietic progenitors, possibly through targeting multiple proapoptotic genes. Bak1 was directly down-regulated by miR-125a and expression of a 3′UTR-less Bak1 blocked miR-125a-induced hematopoietic expansion in vivo. These data demonstrate cell-state-specific regulation by microRNA and identify a unique microRNA functioning to regulate the stem cell pool size.

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Crossref · 10.1073/pnas.0913574107

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  • Impairing mature miR expression by KO of processing machinery in Dicer-KO experiments revealed miR dependency for HSCs and osteoclast development.

    “At a more global level, impairing mature miR expression by KO of the processing machinery in Dicer-KO experiments revealed miR dependency for HSCs 14 and osteoclast development.15”

    From Macrophage development from HSCs requires PU.1-coordinated microRNA expression (2011) · cites this paper as [14]

Citations

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Research works printed but not linked: 32

  1. A Cozzio, et al., Similar MLL-associated leukemias arising from self-renewing stem cells and short-lived myeloid progenitors. Genes Dev 17, 3029–3035 (2003). · DOI
  2. OH Yilmaz, et al., Pten dependence distinguishes haematopoietic stem cells from leukaemia-initiating cells. Nature 441, 475–482 (2006). · DOI · Paper page
  3. J Lu, et al., MicroRNA expression profiles classify human cancers. Nature 435, 834–838 (2005). · DOI
  4. CG Liu, et al., An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Proc Natl Acad Sci USA 101, 9740–9744 (2004). · DOI · Paper page
  5. LF Sempere, NS Sokol, EB Dubrovsky, EM Berger, V Ambros, Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-complex gene activity. Dev Biol 259, 9–18 (2003). · DOI
  6. E Bernstein, AA Caudy, SM Hammond, GJ Hannon, Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363–366 (2001). · DOI
  7. E Bernstein, et al., Dicer is essential for mouse development. Nat Genet 35, 215–217 (2003). · DOI · Paper page
  8. SM Hammond, E Bernstein, D Beach, GJ Hannon, An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404, 293–296 (2000). · DOI
  9. SA Muljo, et al., Aberrant T cell differentiation in the absence of Dicer. J Exp Med 202, 261–269 (2005). · DOI
  10. EP Murchison, JF Partridge, OH Tam, S Cheloufi, GJ Hannon, Characterization of Dicer-deficient murine embryonic stem cells. Proc Natl Acad Sci USA 102, 12135–12140 (2005). · DOI · Paper page
  11. QJ Li, et al., miR-181a is an intrinsic modulator of T cell sensitivity and selection. Cell 129, 147–161 (2007). · DOI
  12. C Xiao, et al., MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131, 146–159 (2007). · DOI
  13. B Zhou, S Wang, C Mayr, DP Bartel, HF Lodish, miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely. Proc Natl Acad Sci USA 104, 7080–7085 (2007). · DOI
  14. F Fazi, et al., A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBPalpha regulates human granulopoiesis. Cell 123, 819–831 (2005). · DOI · Paper page
  15. T Fukao, et al., An evolutionarily conserved mechanism for microRNA-223 expression revealed by microRNA gene profiling. Cell 129, 617–631 (2007). · DOI · Paper page
  16. JB Johnnidis, et al., Regulation of progenitor cell proliferation and granulocyte function by microRNA-223. Nature 451, 1125–1129 (2008). · DOI · Paper page
  17. J Lu, et al., MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors. Dev Cell 14, 843–853 (2008). · DOI · Paper page
  18. R Kühn, F Schwenk, M Aguet, K Rajewsky, Inducible gene targeting in mice. Science 269, 1427–1429 (1995). · DOI · Paper page
  19. R Kumar, V Fossati, M Israel, HW Snoeck, Lin-Sca1+kit- bone marrow cells contain early lymphoid-committed precursors that are distinct from common lymphoid progenitors. J Immunol 181, 7507–7513 (2008). · DOI
  20. K Klarmann, M Ortiz, M Davies, JR Keller, Identification of in vitro growth conditions for c-Kit-negative hematopoietic stem cells. Blood 102, 3120–3128 (2003). · DOI
  21. T Lindsten, et al., The proapoptotic activities of Bax and Bak limit the size of the neural stem cell pool. J Neurosci 23, 11112–11119 (2003). · DOI · Paper page
  22. T Lindsten, et al., The combined functions of proapoptotic Bcl-2 family members bak and bax are essential for normal development of multiple tissues. Mol Cell 6, 1389–1399 (2000). · DOI · Paper page
  23. BS Cobb, et al., A role for Dicer in immune regulation. J Exp Med 203, 2519–2527 (2006). · DOI
  24. L Zhou, et al., Tie2cre-induced inactivation of the miRNA-processing enzyme Dicer disrupts invariant NKT cell development. Proc Natl Acad Sci USA 106, 10266–10271 (2009). · DOI
  25. T Sato, et al., Interferon regulatory factor-2 protects quiescent hematopoietic stem cells from type I interferon-dependent exhaustion. Nat Med 15, 696–700 (2009). · DOI
  26. J Domen, SH Cheshier, IL Weissman, The role of apoptosis in the regulation of hematopoietic stem cells: Overexpression of Bcl-2 increases both their number and repopulation potential. J Exp Med 191, 253–264 (2000). · DOI
  27. C Orelio, et al., The role of apoptosis in the development of AGM hematopoietic stem cells revealed by Bcl-2 overexpression. Blood 103, 4084–4092 (2004). · DOI
  28. JT Opferman, et al., Obligate role of anti-apoptotic MCL-1 in the survival of hematopoietic stem cells. Science 307, 1101–1104 (2005). · DOI
  29. HF Xia, et al., MiR-125b expression affects the proliferation and apoptosis of human glioma cells by targeting Bmf. Cell Physiol Biochem 23, 347–358 (2009). · DOI
  30. MT Le, et al., MicroRNA-125b is a novel negative regulator of p53. Genes Dev 23, 862–876 (2009). · DOI
  31. B Dykstra, et al., Long-term propagation of distinct hematopoietic differentiation programs in vivo. Cell Stem Cell 1, 218–229 (2007). · DOI
  32. M Bousquet, et al., Myeloid cell differentiation arrest by miR-125b-1 in myelodysplastic syndrome and acute myeloid leukemia with the t(2;11)(p21;q23) translocation. J Exp Med 205, 2499–2506 (2008). · DOI

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Metadata

Authors
Guo, S., Lu, J., Schlanger, R., Zhang, H., Wang, J. Y., Fox, M. C., Purton, L. E., Fleming, H. H., Cobb, B., Merkenschlager, M., Golub, T. R., & Scadden, D. T.
Affiliations
  • Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114;
  • Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA 02142;
  • Lymphocyte Development Group, Medical Research Center Clinical Sciences Centre, Imperial College London, London W12 0NN, United Kingdom; and
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2010-07-08
Volume
107
Issue
32
Pages
14229-14234
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.0913574107
Cited by
310
References
6
Language
en

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