Behavioral insensitivity to DEET in Aedes aegypti is a genetically determined trait residing in changes in sensillum function

Stanczyk, Nina M.; Brookfield, John F. Y.; Ignell, Rickard; Logan, James G.; Field, Linda M.

doi:10.1073/pnas.1001313107

Cited by 138Neurobiology and Insect Physiology ResearchInsect Pheromone Research and ControlInsect Pest Control Strategies

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Behavioral insensitivity to DEET in Aedes aegypti is a genetically determined trait residing in changes in sensillum function

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Stanczyk, Nina M., Brookfield, John F. Y., Ignell, Rickard, Logan, James G., Field, Linda M.·2010·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1001313107PMC PMC2889326

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Abstract

N,N -Diethyl- m -toluamide (DEET) is one of the most effective and commonly used mosquito repellents. However, during laboratory trials a small proportion of mosquitoes are still attracted by human odors despite the presence of DEET. In this study behavioral assays identified Aedes aegypti females that were insensitive to DEET, and the selection of either sensitive or insensitive groups of females with males of unknown sensitivity over several generations resulted in two populations with different proportions of insensitive females. Crossing experiments showed the “insensitivity” trait to be dominant. Electroantennography showed a reduced response to DEET in the selected insensitive line compared with the selected sensitive line, and single sensillum recordings identified DEET-sensitive sensilla that were nonresponders in the insensitive line. This study suggests that behavioral insensitivity to DEET in A. aegypti is a genetically determined dominant trait and resides in changes in sensillum function.

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There are several electrophysiological and behavioural assays with correspondingly robust analyses currently being used in vector biology research that could be incorporated into such schemes once adopted by WHOPES and the wider scientific community.
“The promising news is that there are several electrophysiological and behavioural assays with correspondingly robust analyses currently being used in vector biology research that could be incorporated into such schemes once adopted by WHOPES and the wider scientific community [16,36-42].”
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An investigation of DEET-insensitivity in Aedes aegypti
Stanczyk, N. M. (2011) · UPT. Syiah Kuala University Library (Syiah Kuala University) · Cited by 1
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Aedes aegypti Mosquitoes Exhibit Decreased Repellency by DEET following Previous Exposure
Stanczyk, N. M., Brookfield, J. F. Y., Field, L. M., & Logan, J. G. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054438 · Cited by 104
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Investigation of tolerance behavior to N, N-diethylmethylbenzamide (DEET) in populations of Aedes aegypti (Diptera: Culicidae)
Maia, P. C. R. (2020) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)

Authors: Stanczyk, N. M., Brookfield, J. F. Y., Ignell, R., Logan, J. G., & Field, L. M.
Affiliations: University of Nottingham (GB)
Swedish University of Agricultural Sciences (SE)
Rothamsted Research (GB)
ORCID: Brookfield, John F. Y.
Ignell, Rickard
Logan, James G.
Field, Linda M.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2010-05-03
Volume: 107
Issue: 19
Pages: 8575-8580
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1001313107
PMC: PMC2889326
OpenAlex: W2000424479
Cited by: 138
References: 31
Citations by year: 2026: 8 · 2025: 3 · 2024: 8 · 2023: 8 · 2022: 9 · 2021: 5 · 2020: 13 · 2019: 10 · 2018: 7 · 2017: 11 · 2016: 10 · 2015: 3 · 2014: 14 · 2013: 10 · 2012: 5
FWCI: 3.62
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Rothamsted Repository (Rothamsted Repository) (submittedVersion)
LSHTM Research Online (London School of Hygiene and Tropical Medicine) (submittedVersion)
Funders: Svenska Forskningsrådet Formas (IC‐E3)
Vetenskapsrådet
Directorate for Biological Sciences
Biotechnology and Biological Sciences Research Council
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Pheromone Research and Control
Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords: DEET, Aedes aegypti, sensilla, electroantennogram, dominant trait, mosquito olfaction, mosquito vectors
MeSH terms: Aedes, Animals, Animal Structures, Behavior, Animal, Crosses, Genetic, DEET, Female, Insecticide Resistance, Male, Selection, Genetic, Olfactory Receptor Neurons, Quantitative Trait, Heritable

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Behavioral insensitivity to DEET in Aedes aegypti is a genetically determined trait residing in changes in sensillum function

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