Oxytocin and its receptors are synthesized in the rat vasculature
Open accessAbstract
Produced and released by the heart, oxytocin (OT) acts on its cardiac receptors to decrease the cardiac rate and force of contraction. We hypothesized that it might also be produced in the vasculature and regulate vascular tone. Consequently, we prepared acid extracts of the pulmonary artery and vena cava of male rats. OT concentrations in dog and sheep aortae were equivalent to those of rat aorta (2745 ± 180 pg/mg protein), indicating that it is present in the vasculature of several mammalian species. Reverse-phase HPLC of aorta and vena cava extracts revealed a single peak corresponding to the amidated OT nonapeptide. Reverse-transcribed PCR confirmed OT synthesis in these tissues. Using the selective OT receptor ligand compound VI, we detected a high number of OT-binding sites in the rat vena cava and aorta. Furthermore, OT receptor (OTR) mRNA was found in the vena cava, pulmonary vein, and pulmonary artery with lower levels in the aorta, suggesting vessel-specific OTR distribution. The abundance of OTR mRNA in the vena cava and pulmonary vein was associated with high atrial natriuretic peptide mRNA. In addition, we have demonstrated that diethylstilbestrol treatment of immature female rats increased OT significantly in the vena cava but not in the aorta and augmented OTR mRNA in both the aorta (4-fold) and vena cava (2-fold), implying regulation by estrogen. Altogether, these data suggest that the vasculature contains an intrinsic OT system, which may be involved in the regulation of vascular tone as well as vascular regrowth and remodeling.
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- Authors
- Jankowski, M., Wang, D., Hajjar, F., Mukaddam-Daher, S., McCann, S. M., & Gutkowska, J.
- Affiliations
- Pennington Biomedical Research Center (US)
- Centre de recherche, Centre Hospitalier de l'Université de Montréal, Campus Hôtel-Dieu, Montreal, Quebec H2W 1T8, Canada; and Pennington Biomedical Research Center, Louisiana State University, 6400 Perkins Road, Baton Rouge, LA 70808-4124
- Corresponding author
- Gutkowska, Jolanta
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-05-16
- Volume
- 97
- Issue
- 11
- Pages
- 6207-6211
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC18583
- OpenAlex
- W2131682415
- Cited by
- 175
- References
- 28
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 12 · 2020: 7 · 2019: 9 · 2018: 3 · 2017: 5 · 2016: 8 · 2015: 4 · 2014: 6 · 2013: 11 · 2012: 8
- FWCI
- 6.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (MH 51583)
- Topics
- Health Sciences › Medicine › Surgery › Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- oxytocin, oxytocin receptor, vascular tone, vena cava, pulmonary artery, aorta, pulmonary veins, atrial natriuretic peptide, estrogen regulation, diethylstilbestrol
- MeSH terms
- Animals, Aorta, Blood Vessels, Chromatography, High Pressure Liquid, Diethylstilbestrol, Dogs, Estrogens, Female, Gene Expression Regulation, Atrial Natriuretic Factor, Organ Specificity, Oxytocin, Pulmonary Artery, Pulmonary Veins, RNA, Messenger, Sheep, Species Specificity, Vena Cava, Superior, Polymerase Chain Reaction, Rats, Sprague-Dawley, Receptors, Oxytocin, Rats
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