The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Akt1 and Akt2 protein kinases differentially contribute to macrophage polarization
Open accessAbstract
Activated macrophages are described as classically activated or M1 type and alternatively activated or M2 type, depending on their response to proinflammatory stimuli and the expression of genetic markers including iNOS, arginase1, Ym1, and Fizz1. Here we report that Akt kinases differentially contribute to macrophage polarization, with Akt1 ablation giving rise to an M1 and Akt2 ablation resulting in an M2 phenotype. Accordingly, Akt2 −/− mice were more resistant to LPS-induced endotoxin shock and to dextran sulfate sodium (DSS)-induced colitis than wild-type mice, whereas Akt1 −/− mice were more sensitive. Cell depletion and reconstitution experiments in a DSS-induced colitis model confirmed that the effect was macrophage-dependent. Gene-silencing studies showed that the M2 phenotype of Akt2 −/− macrophages was cell autonomous. The microRNA miR-155, whose expression was repressed in naive and in LPS-stimulated Akt2 −/− macrophages, and its target C/EBPβ appear to play a key role in this process. C/EBPβ, a hallmark of M2 macrophages that regulates Arg1, was up-regulated upon Akt2 ablation or silencing. Overexpression or silencing of miR-155 confirmed its central role in Akt isoform-dependent M1/M2 polarization of macrophages.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Protective and pathogenic functions of macrophage subsets
Murray, P. J., & Wynn, T. A. (2011) · Nature Reviews Immunology · doi:10.1038/nri3073 · Cited by 5,269
Alternative activation of macrophages: mechanism and functions
Gordon, S., & Martinez, F. O. (2010) · Immunity · doi:10.1016/j.immuni.2010.05.007 · Cited by 4,197
Transcriptional regulation of macrophage polarization: enabling diversity with identity
Lawrence, T., & Natoli, G. (2011) · Nature Reviews Immunology · doi:10.1038/nri3088 · Cited by 2,284
Adiponectin Promotes Macrophage Polarization toward an Anti-inflammatory Phenotype
Ohashi, K., Parker, J. L., Ouchi, N., Higuchi, A., Vita, J. A., Gokce, N., Pedersen, A. A., Kalthoff, C., Tullin, S., Sams, A., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.088708 · Cited by 646 · cited 1×
“Leptin and adiponectin also regulate arginase expression and macrophage polarization to the M2 phenotype (16).”
BackgroundRuffell, D., Mourkioti, F., Gambardella, A., Kirstetter, P., Lopez, R. G., Rosenthal, N., & Nerlov, C. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908641106 · Cited by 626
The Kinase Akt1 Controls Macrophage Response to Lipopolysaccharide by Regulating MicroRNAs
Androulidaki, A., Iliopoulos, D., Arranz, A., Doxaki, C., Schworer, S., Zacharioudaki, V., Margioris, A. N., Tsichlis, P. N., & Tsatsanis, C. (2009) · Immunity · doi:10.1016/j.immuni.2009.06.024 · Cited by 581
Self-citationProtein Kinase Bα/Akt1 Regulates Placental Development and Fetal Growth
Yang, Z.-Z., Tschopp, O., Hemmings-Mieszczak, M., Feng, J., Brodbeck, D., Perentes, E., & Hemmings, B. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m302847200 · Cited by 384
Cited by
Macrophage activation and polarization: nomenclature and experimental guidelines
Murray, P. J., Allen, J. E., Biswas, S. K., Fisher, E. A., Gilroy, D. W., Goerdt, S., Gordon, S., Hamilton, J. A., Ivashkiv, L. B., Lawrence, T., et al. (2014) · Immunity · doi:10.1016/j.immuni.2014.06.008 · Cited by 6,337
AKT/PKB Signaling: Navigating the Network
Manning, B. D., & Toker, A. (2017) · Cell · doi:10.1016/j.cell.2017.04.001 · Cited by 3,772
Murray, P. J. (2017) · Annual Review of Physiology · doi:10.1146/annurev-physiol-022516-034339 · Cited by 2,981
The metabolic signature of macrophage responses
Viola, A., Munari, F., Sánchez-Rodríguez, R., Scolaro, T., & Castegna, A. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.01462 · Cited by 2,021
Macrophage Polarization States in the Tumor Microenvironment
Boutilier, A. J., & Elsawa, S. F. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22136995 · Cited by 1,631
Akt Signaling Pathway in Macrophage Activation and M1/M2 Polarization
Vergadi, E., Ieronymaki, E., Lyroni, K., Vaporidi, K., & Tsatsanis, C. (2017) · The Journal of Immunology · doi:10.4049/jimmunol.1601515 · Cited by 1,148
Self-citationZhou, W., Ke, S. Q., Huang, Z., Flavahan, W., Fang, X., Paul, J., Wu, L., Sloan, A. E., McLendon, R. E., Li, X., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3090 · Cited by 940
Zhou, D., Huang, C., Lin, Z., Zhan, S., Kong, L., Fang, C., & Li, J. (2014) · Cellular Signalling · doi:10.1016/j.cellsig.2013.11.004 · Cited by 778
Mechanically activated ion channel Piezo1 modulates macrophage polarization and stiffness sensing
Atcha, H., Jairaman, A., Holt, J. R., Meli, V. S., Nagalla, R. R., Veerasubramanian, P. K., Brumm, K. T., Lim, H. E., Othy, S., Cahalan, M. D., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23482-5 · Cited by 644
Microbial metabolite butyrate facilitates M2 macrophage polarization and function
Ji, J., Shu, D., Zheng, M., Wang, J., Luo, C., Wang, Y., Guo, F., Zou, X., Lv, X., Li, Y., et al. (2016) · Scientific Reports · doi:10.1038/srep24838 · Cited by 334
Wang, D., Wang, X., Si, M., Yang, J., Sun, S., Wu, H., Cui, S., Qu, X., & Yu, X. (2020) · Cancer Letters · doi:10.1016/j.canlet.2020.01.005 · Cited by 322
MicroRNA-155 Promotes Autophagy to Eliminate Intracellular Mycobacteria by Targeting Rheb
Wang, J., Yang, K., Zhou, L., MinhaoWu, Wu, Y., Zhu, M., Lai, X., Chen, T., Feng, L., Li, M., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003697 · Cited by 265
miR-125a-5p Regulates Differential Activation of Macrophages and Inflammation
Banerjee, S., Cui, H., Xie, N., Tan, Z., Yang, S., Icyuz, M., Thannickal, V. J., Abraham, E., & Liu, G. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.426866 · Cited by 258
Wang, Z., Brandt, S., Medeiros, A., Wang, S., Wu, H., Dent, A., & Serezani, C. H. (2015) · PLOS ONE · doi:10.1371/journal.pone.0115855 · Cited by 169
MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264.7 macrophages
Huang, C., Liu, X.-J., QunZhou, Xie, J., Ma, T.-T., Meng, X.-M., & Li, J. (2016) · International Immunopharmacology · doi:10.1016/j.intimp.2016.01.009 · Cited by 160
Wang, X., Ji, Y., Feng, P., Liu, R., Li, G., Zheng, J., Xue, Y., Wei, Y., Ji, C., Chen, D., et al. (2021) · Advanced Science · doi:10.1002/advs.202100209 · Cited by 147
Control of the Inflammatory Macrophage Transcriptional Signature by miR-155
Jablonski, K. A., Gaudet, A. D., Amici, S. A., Popovich, P. G., & Guerau-de-Arellano, M. (2016) · PLOS ONE · doi:10.1371/journal.pone.0159724 · Cited by 146
Neuroinflammatory changes negatively impact on LTP: A focus on IL-1β
Lynch, M. A. (2015) · Brain Research · doi:10.1016/j.brainres.2014.08.040 · Cited by 115
Festuccia, W. T., Pouliot, P., Bakan, I., Sabatini, D. M., & Laplante, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0095432 · Cited by 103
Bi, J., Zeng, X., Zhao, L., Wei, Q., Yu, L., Wang, X., Yu, Z., Cao, Y., Shan, F., & Wei, M. (2016) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2016.71 · Cited by 63
RetractedNagenborg, J., Goossens, P., Biessen, E. A. L., & Donners, M. M. P. C. (2017) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2017.10.005 · Cited by 50
Su, S.-B., Qin, S.-Y., Xian, X.-L., Huang, F.-F., Huang, Q.-L., ZhangDi, H.-J., & Jiang, H.-X. (2021) · Life Sciences · doi:10.1016/j.lfs.2020.118677 · Cited by 40
Atta, A. A., Ibrahim, W. W., Mohamed, A. F., & Abdelkader, N. F. (2023) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2023.175810 · Cited by 29
Ko, J. H., & Oh, J. Y. (2023) · Cytotherapy · doi:10.1016/j.jcyt.2023.03.013 · Cited by 3
Metadata
- Authors
- Arranz, A., Doxaki, C., Vergadi, E., Martinez de la Torre, Y., Vaporidi, K., Lagoudaki, E. D., Ieronymaki, E., Androulidaki, A., Venihaki, M., Margioris, A. N., Stathopoulos, E. N., Tsichlis, P. N., & Tsatsanis, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-05-30
- Volume
- 109
- Issue
- 24
- Pages
- 9517-9522
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1981056356
- Cited by
- 608
- References
- 7
- FWCI
- 12.82
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
