MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264.7 macrophages
Open accessAbstract
Macrophages are heterogeneous and plastic cells which are able to undergo dynamic transition between M1 and M2 polarized phenotypes in response to the microenvironment signals. However, the underlying molecular mechanisms of macrophage polarization are still obscure. In the current study, it was revealed that miR-146a might play a pivotal role in macrophage polarization. As our results indicated, miR-146a was highly expressed in M2 macrophages rather than M1 macrophages. Over-expression of miR-146a resulted in significantly decreased production of pro-inflammatory cytokines including iNOS and TNF-α in M1 macrophages, while increased production of M2 marker genes such as Arg1 and CD206 in M2 macrophages. In contrast, knockdown of miR-146a promoted M1 macrophage polarization but diminished M2 macrophage polarization. Mechanistically, it was revealed that miR-146a modulated macrophage polarization by targeting Notch1. Of note, PPARγ was responsible as another target for miR-146a-mediated macrophage polarization. Taken together, it was suggested that miR-146a might serve as a molecular regulator in macrophage polarization and is a potential therapeutic target for inflammatory diseases.
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miR-146a promotes polarization towards the M2 phenotype.
“Huang et al. also reported that miR-146a promotes polarization towards the M2 phenotype [152].”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [152]
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- Authors
- Huang, C., Liu, X.-J., QunZhou, Xie, J., Ma, T.-T., Meng, X.-M., & Li, J.
- Affiliations
- Anhui Medical University (CN)
- Corresponding author
- Li, Jun
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2016-03
- Volume
- 32
- Pages
- 46-54
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W2279015277
- Cited by
- 160
- References
- 48
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 17 · 2023: 20 · 2022: 29 · 2021: 26 · 2020: 23 · 2019: 11 · 2018: 9 · 2017: 7 · 2016: 3
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- International Immunopharmacology (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundations of China (81473268, 81273526)
- Specialized Research Fund for the Doctoral Program of Higher Education of China (20123420120001)
- Anhui Provincial Natural Science Foundation (1408085MKL31, 1308085MH145)
- Foundation of Anhui Medical University (XJ201118)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Immunology and Microbiology › Immunology › Macrophage Migration Inhibitory Factor
- Keywords
- miR-146a, macrophage polarization, Notch1 signaling, M1/M2 macrophages, PPARγ, RAW 264.7 macrophages, inflammatory cytokines, inducible nitric oxide synthase, TNF-α, Arg1, CD206, microRNA target regulation, inflammatory diseases
- MeSH terms
- Animals, Cell Line, Macrophages, Tumor Necrosis Factor-alpha, Interleukin-10, MicroRNAs, PPAR gamma, Mice, Receptor, Notch1
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