Cited by 108Pancreatic function and diabetesDiabetes and associated disordersPancreatic and Hepatic Oncology Research

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Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel

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Jin, Liang, Feng, Tao, Shih, Hung Ping, Zerda, Ricardo, Luo, Angela, Hsu, Jasper, Mahdavi, Alborz, Sander, Maike, Tirrell, David A., Riggs, Arthur D., Ku, Hsun Teresa·2013·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1301889110PMC PMC3593860

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Abstract

The study of hematopoietic colony-forming units using semisolid culture media has greatly advanced the knowledge of hematopoiesis. Here we report that similar methods can be used to study pancreatic colony-forming units. We have developed two pancreatic colony assays that enable quantitative and functional analyses of progenitor-like cells isolated from dissociated adult (2–4 mo old) murine pancreas. We find that a methylcellulose-based semisolid medium containing Matrigel allows growth of duct-like “Ring/Dense” colonies from a rare ( ∼ 1%) population of total pancreatic single cells. With the addition of roof plate-specific spondin 1, a wingless-int agonist, Ring/Dense colony-forming cells can be expanded more than 100,000-fold when serially dissociated and replated in the presence of Matrigel. When cells grown in Matrigel are then transferred to a Matrigel-free semisolid medium with a unique laminin-based hydrogel, some cells grow and differentiate into another type of colony, which we name “Endocrine/Acinar.” These Endocrine/Acinar colonies are comprised mostly of endocrine- and acinar-like cells, as ascertained by RNA expression analysis, immunohistochemistry, and electron microscopy. Most Endocrine/Acinar colonies contain beta-like cells that secrete insulin/C-peptide in response to D -glucose and theophylline. These results demonstrate robust self-renewal and differentiation of adult Ring/Dense colony-forming units in vitro and suggest an approach to producing beta-like cells for cell replacement of type 1 diabetes. The methods described, which include microfluidic expression analysis of single cells and colonies, should also advance study of pancreas development and pancreatic progenitor cells.

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Authors: Jin, L., Feng, T., Shih, H. P., Zerda, R., Luo, A., Hsu, J., Mahdavi, A., Sander, M., Tirrell, D. A., Riggs, A. D., & Ku, H. T.
Affiliations: Department of Diabetes and Metabolic Diseases Research,
University of California San Diego (US)
Electron Microscopy Core, and
Department of Bioengineering, and
California Institute of Technology (US)
City Of Hope National Medical Center (US)
ORCID: Shih, Hung Ping
Hsu, Jasper
Mahdavi, Alborz
Sander, Maike
Tirrell, David A.
Riggs, Arthur D.
Ku, Hsun Teresa
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2013-02-19
Volume: 110
Issue: 10
Pages: 3907-3912
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1301889110
PMC: PMC3593860
OpenAlex: W2160112404
Cited by: 108
References: 60
Citations by year: 2026: 1 · 2025: 7 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 11 · 2017: 8 · 2016: 14 · 2015: 9 · 2014: 15 · 2013: 6
FWCI: 14.45
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
CaltechAUTHORS (California Institute of Technology) (submittedVersion)
MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
Funders: National Cancer Institute (P30CA-33572, P30CA033572)
National Institute of Diabetes and Digestive and Kidney Diseases (DK078803, R01DK081587, R01DK078803, U01DK089533)
Topics: Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
Keywords: pancreatic progenitor cells, Matrigel, beta-like cells, insulin secretion, self-renewal, pancreatic development, semi-solid culture
MeSH terms: Animals, Cell Differentiation, Collagen, Colony-Forming Units Assay, Culture Media, Drug Combinations, Islets of Langerhans, Laminin, Mice, Inbred C57BL, Mice, Transgenic, Nerve Tissue Proteins, Pancreas, Proteoglycans, Hydrogels, Microscopy, Electron, Transmission, Insulin-Secreting Cells, Mice, Basic Helix-Loop-Helix Proteins, Wnt Signaling Pathway, Acinar Cells

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Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel

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