Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition
Open accessAbstract
In the representative gut bacterium Lactobacillus plantarum, we identified genes encoding the enzymes involved in a saturation metabolism of polyunsaturated fatty acids and revealed in detail the metabolic pathway that generates hydroxy fatty acids, oxo fatty acids, conjugated fatty acids, and partially saturated trans-fatty acids as intermediates. Furthermore, we observed these intermediates, especially hydroxy fatty acids, in host organs. Levels of hydroxy fatty acids were much higher in specific pathogen-free mice than in germ-free mice, indicating that these fatty acids are generated through polyunsaturated fatty acids metabolism of gastrointestinal microorganisms. These findings suggested that lipid metabolism by gastrointestinal microbes affects the health of the host by modifying fatty acid composition.
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CLA has functional properties and potential health-promoting effects such as anticarcinogenic, anti-inflammatory, reducing body fat deposition, reducing atherosclerosis, modulating immune system, reducing blood glucose, reducing osteoporosis, preventing cardiovascular disease, controlling serum cholesterol and triglycerides, and improving insulin resistance.
“CLA has several functional properties and potential health-promoting effects, such as anticarcinogenic, anti-inflammatory, reducing and preventing body fat deposition, reducing atherosclerosis [261,262], modulating the immune system [263], reducing blood glucose levels [264], reducing osteoporosis [265], preventing and treating cardiovascular disease, controlling serum levels of cholesterol and”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [261]
The Introduction (page not provided) says that CLA reduces carcinogenesis, atherosclerosis, and body fat, supporting three effects in the portion associated with this citation. It does not report an anti-inflammatory effect or specifically establish prevention of body-fat deposition. The later effects in the citing sentence are not assigned to this citation. (LLM, conf=0.88)
Experimental — accuracy not yet measuredL. rhamnosus can generate and isomerize cLA and exert anti-obesity effects in diet-induced obese mice in a cLA-dependent manner.
“to generate and isomerize cLA and exert anti-obesity effects in diet-induced obese mice in a cLA-dependent manner[279,403,415,416]”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [279]
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- Authors
- Kishino, S., Takeuchi, M., Park, S.-B., Hirata, A., Kitamura, N., Kunisawa, J., Kiyono, H., Iwamoto, R., Isobe, Y., Arita, M., Arai, H., Ueda, K., Shima, J., Takahashi, S., Yokozeki, K., Shimizu, S., & Ogawa, J.
- Affiliations
- Kyoto University (JP)
- Division of Applied Life Sciences and
- Institute of Medical Science, The University of Tokyo (JP)
- Business–Academia Collaborative Laboratory and
- The University of Tokyo (JP)
- Institute for Integrated Cell-Material Sciences, Kyoto University (JP)
- Kyoto University of Advanced Science (JP)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2013-10-14
- Volume
- 110
- Issue
- 44
- Pages
- 17808-17813
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3816446
- OpenAlex
- W1984238073
- Cited by
- 395
- References
- 33
- Citations by year
- 2026: 30 · 2025: 36 · 2024: 30 · 2023: 29 · 2022: 44 · 2021: 42 · 2020: 44 · 2019: 39 · 2018: 35 · 2017: 22 · 2016: 20 · 2015: 14 · 2014: 9
- FWCI
- 6.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (24658079, 23229004, 23248014)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Keywords
- Lactobacillus plantarum, hydroxy fatty acids, oxo fatty acids, conjugated fatty acids, trans fatty acids, gut microbiota, germ-free mice, host-microbe interactions
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Chromatography, Liquid, Cloning, Molecular, Fatty Acids, Unsaturated, Multigene Family, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases, Specific Pathogen-Free Organisms, Sequence Homology, Sequence Analysis, DNA, DNA Primers, Gastrointestinal Tract, Lactiplantibacillus plantarum, Lipid Metabolism, Mice, Tandem Mass Spectrometry, Metabolic Networks and Pathways
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