Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors
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Significance: Monitoring human organ-on-a-chip systems presents a significant challenge, where the capability of in situ continual monitoring of organ behaviors and their responses to pharmaceutical compounds over extended periods of time is critical in understanding the dynamics of drug effects and therefore accurate prediction of human organ reactions. In this work, we report a fully integrated modular physical, biochemical, and optical sensing platform, interfaced through a fluidics-routing breadboard with a multi–organ-on-a-chip system to achieve in situ, continual, and automated sensing of microenvironment biophysical and biochemical parameters. It is anticipated that our platform technology that is conveniently compatible with existing organ-on-a-chip models will potentially enhance their performance in drug screening by providing a multitude of sensing data not previously available.
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A two-organ system has been simulated using connected chambers containing different tissue constructs to mimic liver injury and Type-2 diabetes.
“The two-organ system has been simulated using connected chambers containing different tissue constructs to mimic liver injury (with intestinal epithelial cells and liver cells)101 and Type-2 diabetes (with human pancreatic islets and liver spheroids)102.”
From Organoids (2022) · cites this paper as [102]
Citation 102 is attached to the Type-2 diabetes example involving pancreatic islets and liver spheroids. The “Fully Integrated Human Liver (Cancer)-and Heart-on-Chips” section instead describes linked heart and liver organoids used to study drug responses and toxicity, not a pancreatic-islet–liver model of diabetes. No page number is available in our copy. (LLM, conf=0.88)
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- Authors
- Zhang, Y. S., Aleman, J., Shin, S. R., Kilic, T., Kim, D., Mousavi Shaegh, S. A., Massa, S., Riahi, R., Chae, S., Hu, N., Avci, H., Zhang, W., Silvestri, A., Sanati Nezhad, A., Manbohi, A., De Ferrari, F., Polini, A., Calzone, G., Shaikh, N., Alerasool, P., Budina, E., Kang, J., Bhise, N., Ribas, J., Pourmand, A., Skardal, A., Shupe, T., Bishop, C. E., Dokmeci, M. R., Atala, A., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- Biomaterials Innovation Research Center, Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139;
- Sahand University of Technology (IR)
- Wake Forest University Health Sciences (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-03-06
- Volume
- 114
- Issue
- 12
- Pages
- E2293-E2302
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5373350
- OpenAlex
- W2593758551
- Cited by
- 833
- References
- 5
- Citations by year
- 2026: 54 · 2025: 81 · 2024: 100 · 2023: 98 · 2022: 104 · 2021: 133 · 2020: 98 · 2019: 85 · 2018: 60 · 2017: 19 · 2014: 1
- FWCI
- 32.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- PORTO@Iris (submittedVersion)
- PORTO Publications Open Repository TOrino (Politecnico di Torino) (submittedVersion)
- Funders
- HHS | National Institutes of Health (NIH) (1K99CA201603-01A1, EB012597, AR057837, DE021468, HL099073, R56AI105024, AR068258, AR066193, EB022403, EB021148)
- DOD | Defense Threat Reduction Agency (DTRA) (N66001-13-C-2027)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- organ-on-a-chip, organoids, multi-sensor integration, in situ monitoring, microfluidics, automated monitoring, real-time sensing, drug screening, optical sensing
- MeSH terms
- Automation, Drug Evaluation, Preclinical, Heart, Humans, Liver, Models, Biological, Myocardium, Organoids, Biosensing Techniques, Microfluidics
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