The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts.
Open accessAbstract
Using a quantitative electron microscopic autoradiographic technique, we have localized the initial binding step of 125I-labeled epidermal growth factor (125I-EGF) to the plasma membrane of the human fibroblast. After initial binding, labeled EGF is internalized progressively by the cell in a time- and temperature-dependent fashion; when cell-associated radioactivity comes to steady state, approximately 1/3 of the autoradiographic grains are related to the plasma membrane and approximately 2/3 have been internalized. Under these conditions the internalized grains are almost exclusively related to lysosomal structures. When 125I-EGF associates with the cells for 2 hr at 4 degrees or 2 min at 37 degrees, 34% of grains localize to coated regions of the membrane. These coated regions make up less than 2% of the membrane surface. These data directly confirm kinetic studies and suggest that saturable binding of EGF is followed by adsorptive pinocytosis and cellular degradation of the ligand and possibly its cell surface receptor.
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Cites
Cohen, S. (1962) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83739-0 · Cited by 2,193
Self-citationYOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L
Roth, T. F., & Porter, K. R. (1964) · The Journal of Cell Biology · doi:10.1083/jcb.20.2.313 · Cited by 1,318
Carpenter, G., & Cohen, S. (1976) · The Journal of cell biology · doi:10.1083/jcb.71.1.159 · Cited by 1,286
Self-citationSilverstein, S. C., Steinman, R. M., & Cohn, Z. A. (1977) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.46.070177.003321 · Cited by 1,286
Gavin, J. R., Roth, J., Neville, D. M., De Meyts, P., & Buell, D. N. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.1.84 · Cited by 1,040
Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.
Pearse, B. M. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.4.1255 · Cited by 683
Human epidermal growth factor: isolation and chemical and biological properties.
Cohen, S., & Carpenter, G. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.4.1317 · Cited by 509
Self-citationAnderson, R. G. W., Brown, M. S., & Goldstein, J. L. (1977) · Cell · doi:10.1016/0092-8674(77)90022-8 · Cited by 489
Characterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts
Carpenter, G., Lembach, K., Morrison, M., & Cohen, S. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41417-8 · Cited by 435
Self-citationBinding and degradation of 125I-insulin by rat hepatocytes.
Terris, S., & Steiner, D. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40770-9 · Cited by 421
Cited by
Coated pits, coated vesicles, and receptor-mediated endocytosis
Goldstein, J. L., Anderson, R. G. W., & Brown, M. S. (1979) · Nature · doi:10.1038/279679a0 · Cited by 1,532
Receptor downregulation and multivesicular-body sorting
Katzmann, D. J., Odorizzi, G., & Emr, S. D. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm973 · Cited by 1,201
Biogenesis and Function of Multivesicular Bodies
Piper, R. C., & Katzmann, D. J. (2007) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.23.090506.123319 · Cited by 771
THE ESCRT COMPLEXES: Structure and Mechanism of a Membrane-Trafficking Network
Hurley, J. H., & Emr, S. D. (2006) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.35.040405.102126 · Cited by 546
Receptor-mediated endocytosis: insights from the lipoprotein receptor system
Brown, M. S., & Goldstein, J. L. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.7.3330 · Cited by 506
Metadata
- Authors
- Gorden, P., Carpentier, J. L., Cohen, S., & Orci, L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-10
- Volume
- 75
- Issue
- 10
- Pages
- 5025-5029
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2029287429
- Cited by
- 351
- References
- 15
- FWCI
- 18.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Gorden, P., Carpentier, J. L., Cohen, S., & Orci, L. (1978). Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.10.5025
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
