Cited by 109Virus-based gene therapy researchT-cell and Retrovirus StudiesHIV Research and Treatment

The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.

Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration

Open access

Fujiwara, T, Craigie, R·1989·Proceedings of the National Academy of Sciences

Open this paper in CoChat — free account

Abstract

After retroviral infection of a permissive cell, the viral RNA is reverse-transcribed to make a DNA copy of the viral genome. Integration of this DNA copy into the host genome is a necessary step for efficient viral replication. We have developed a cell-free system for integration of exogenous mini-retroviral DNA. The termini of this linear mini-Moloney murine leukemia virus (MoMLV) DNA are designed to mimic the ends of authentic unintegrated MoMLV DNA. The viral proteins required for integration can be provided either as a cytoplasmic extract of MoMLV-infected NIH 3T3 cells or as disrupted MoMLV particles. Phage lambda DNA serves as the target for integration. Genetic markers present on the mini-MoMLV DNA enable integration events to be detected, and the recombinants recovered, by selection in Escherichia coli. Integration, which occurs at heterogeneous locations in the target DNA, is absolutely dependent on the presence of a source of viral proteins and a divalent cation in the reaction mixture. The fidelity of the integration reaction was confirmed by sequencing the junctions between the integrated MoMLV DNA and adjacent lambda DNA sequence. In each case, as expected for authentic MoMLV DNA integration, a 4-base-pair duplication of target DNA sequence flanked the integrated MoMLV DNA.

Integrity signals

Registry

Crossref · 10.1073/pnas.86.9.3065

Cites

13 of 13 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Correct integration of retroviral DNA in vitro
Brown, P. O., Bowerman, B. A., VARMUS, H. E., & Bishop, J. M. (1987) · Cell · doi:10.1016/0092-8674(87)90287-x · Cited by 460
Retroviral DNA integration: structure of an integration intermediate
Fujiwara, T., & Mizuuchi, K. (1988) · Cell · doi:10.1016/0092-8674(88)90071-2 · Cited by 385
Self-citation
Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: implications for retrovirus integration.
Shoemaker, C. B., Goff, S. P., Gilboa, E., Paskind, M. P., Mitra, S. W., & Baltimore, D. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.7.3932 · Cited by 259
Construction and analysis of deletion mutations in the pol gene of Moloney murine leukemia virus: a new viral function required for productive infection
Schwartzberg, P., Colicelli, J., & Goff, S. P. (1984) · Cell · doi:10.1016/0092-8674(84)90439-2 · Cited by 242
RNA metabolism of murine leukemia virus: Detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA
Fan, H., & Baltimore, D. (1973) · Journal of Molecular Biology · doi:10.1016/0022-2836(73)90235-0 · Cited by 221
Sequence and spacing requirements of a retrovirus integration site
Colicelli, J., & Goff, S. P. (1988) · Journal of Molecular Biology · doi:10.1016/0022-2836(88)90378-6 · Cited by 198
Mechanism of transposition of bacteriophage Mu: structure of a transposition intermediate
Craigie, R., & Mizuuchi, K. (1985) · Cell · doi:10.1016/s0092-8674(85)80067-2 · Cited by 155
Self-citation
Circles with two tandem LTRs are precursors to integrated retrovirus DNA
Panganiban, A. T., & Temin, H. M. (1984) · Cell · doi:10.1016/0092-8674(84)90347-7 · Cited by 138
Mutants and pseudorevertants of moloney murine leukemia virus with alterations at the integration site
Colicelli, J., & Goff, S. P. (1985) · Cell · doi:10.1016/0092-8674(85)90114-x · Cited by 137
Avian sarcoma and leukosis virus pol-endonuclease recognition of the tandem long terminal repeat junction: minimum site required for cleavage is also required for viral growth
Cobrinik, D. E., Katz, R. A., Terry, R., Skalka, A. M., & Leis, J. P. (1987) · Journal of Virology · doi:10.1128/jvi.61.6.1999-2008.1987 · Cited by 63

Show all 13 references

Sorted by citations

HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
Engelman, A., Mizuuchi, K., & Craigie, R. (1991) · Cell · doi:10.1016/0092-8674(91)90297-c · Cited by 655
Self-citation
The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro
Craigie, R., Fujiwara, T., & Bushman, F. (1990) · Cell · doi:10.1016/0092-8674(90)90126-y · Cited by 409
Self-citation
HIV-1 cDNA Integration: Requirement of HMG I(Y) Protein for Function of Preintegration Complexes In Vitro
Farnet, C. M., & Bushman, F. D. (1997) · Cell · doi:10.1016/s0092-8674(00)81888-7 · Cited by 359
The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro
Katz, R. A., Merkel, G., Kulkosky, J., Leis, J., & Skalka, A. M. (1990) · Cell · doi:10.1016/0092-8674(90)90290-u · Cited by 346
Retroviral DNA integration directed by HIV integration protein in vitro
Bushman, F. D., Fujiwara, T., & Craigie, R. (1990) · Science · doi:10.1126/science.2171144 · Cited by 338
Self-citation
Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity.
Sherman, P. A., & Fyfe, J. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.13.5119 · Cited by 334
HIV DNA Integration
Craigie, R., & Bushman, F. D. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006890 · Cited by 327
Self-citation
Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells
Wiskerchen, M. A., & Muesing, M. A. (1995) · Journal of Virology · doi:10.1128/jvi.69.1.376-386.1995 · Cited by 271
Retroviral DNA Integration
Lesbats, P., Engelman, A., & Cherepanov, P. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00125 · Cited by 250
GENETICS OF RETROVIRAL INTEGRATION
Goff, S. P. (1992) · Annual Review of Genetics · doi:10.1146/annurev.ge.26.120192.002523 · Cited by 224
Tn552, a novel transposable element from Staphylococcus aureus
Rowland, S., & Dyke, K. G. H. (1990) · Molecular Microbiology · doi:10.1111/j.1365-2958.1990.tb00669.x · Cited by 224
Bacterial transposases and retroviral integrases
Polard, P., & Chandler, M. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.tb02217.x · Cited by 206
Ty3 integrates within the region of RNA polymerase III transcription initiation.Preprint
Chalker, D. L., & Sandmeyer, S. B. (1992) · Genes & Development · doi:10.1101/gad.6.1.117 · Cited by 208
Retroviral DNA Integration
Hindmarsh, P. L., & Leis, J. P. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.4.836-843.1999 · Cited by 203
Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro.
Leavitt, A. D., Shiue, L., & VARMUS, H. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53969-7 · Cited by 200
Human immunodeficiency virus integrase protein requires a subterminal position of its viral DNA recognition sequence for efficient cleavage
Vink, C., van Gent, D. C., Elgersma, Y., & Plasterk, R. H. A. (1991) · Journal of Virology · doi:10.1128/jvi.65.9.4636-4644.1991 · Cited by 181
Human immunodeficiency virus integration in a cell-free system
Ellison, V., Abrams, H. D., Roe, T., Lifson, J. D., & Brown, P. O. (1990) · Journal of Virology · doi:10.1128/jvi.64.6.2711-2715.1990 · Cited by 179
Integration of Retroviral DNA
Brown, P. O. (1990) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-75218-6_2 · Cited by 163
Fluorescent Pteridine Nucleoside Analogs: A Window on DNA Interactions
Hawkins, M. E. (2001) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:34:2:257 · Cited by 153
Bromo- and Extraterminal Domain Chromatin Regulators Serve as Cofactors for Murine Leukemia Virus Integration
Gupta, S. S., Maetzig, T., Maertens, G. N., Sharif, A., Rothe, M., Weidner-Glunde, M., Galla, M., Schambach, A., Cherepanov, P., & Schulz, T. F. (2013) · Journal of Virology · doi:10.1128/jvi.01942-13 · Cited by 139
Both substrate and target oligonucleotide sequences affect in vitro integration mediated by human immunodeficiency virus type 1 integrase protein produced in Saccharomyces cerevisiae
Leavitt, A. D., Rose, R. B., & Varmus, H. E. (1992) · Journal of Virology · doi:10.1128/jvi.66.4.2359-2368.1992 · Cited by 126
Distribution of targets for avian retrovirus DNA integration in vivo
Withers-Ward, E. S., Kitamura, Y., Barnes, J. P., & Coffin, J. M. (1994) · Genes & Development · doi:10.1101/gad.8.12.1473 · Cited by 125
Retroviral Mutagenesis of Cellular Oncogenes: A Review with Insights into the Mechanisms of Insertional Activation
Kung, H. J., Boerkoel, C. F., & Carter, T. H. (1991) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-76524-7_1 · Cited by 117
Processing of Viral DNA Ends Channels the HIV-1 Integration Reaction to Concerted Integration*[boxs]
Li, M., & Craigie, R. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m505367200 · Cited by 107
Self-citation
Human immunodeficiency virus type 1 integration protein: DNA sequence requirements for cleaving and joining reactions
Sherman, P. A., Dickson, M. L., & Fyfe, J. A. (1992) · Journal of Virology · doi:10.1128/jvi.66.6.3593-3601.1992 · Cited by 107
Moloney murine leukemia virus-derived retroviral vectors decay intracellularly with a half-life in the range of 5.5 to 7.5 hours
Andreadis, S. T., Brott, D., Fuller, A. O., & Palsson, B. Ø. (1997) · Journal of Virology · doi:10.1128/jvi.71.10.7541-7548.1997 · Cited by 101
Incorporation of a fluorescent guanosine analog into olignoucleotides and its application to a real time assay for the HIV-1 integrase 3′-Processing reaction
Hawkins, M. E., Pfieiderer, W., Mazumder, A., Pommier, Y. G., & Balis, F. M. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.15.2872 · Cited by 91
Transposase‐induced excision and circularization of the bacterial insertion sequence IS911.
Polard, P., Prère, M. F., Fayet, O., & Chandler, M. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05615.x · Cited by 91
Overlapping retrovirus U5 sequence elements are required for efficient integration and initiation of reverse transcription
Cobrinik, D. E., Aiyar, A. A., Ge, Z., Katzman, M., Huang, H., & Leis, J. P. (1991) · Journal of Virology · doi:10.1128/jvi.65.7.3864-3872.1991 · Cited by 89
Localization of DNA Binding Activity of HIV-1 Integrase to the C-Terminal Half of the Protein
Woerner, A. M., Klutch, M. J., Levin, J. G., & Marcus-Sekura, C. J. (1992) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1992.8.297 · Cited by 86
Concerted integration of linear retroviral DNA by the avian sarcoma virus integrase in vitro: dependence on both long terminal repeat termini
Aiyar, A. A., Hindmarsh, P., Skalka, A. M., & Leis, J. P. (1996) · Journal of Virology · doi:10.1128/jvi.70.6.3571-3580.1996 · Cited by 85
A mutation at one end of Moloney murine leukemia virus DNA blocks cleavage of both ends by the viral integrase in vivo
Murphy, J. E., & Goff, S. P. (1992) · Journal of Virology · doi:10.1128/jvi.66.8.5092-5095.1992 · Cited by 83
Fv-1 restriction and its effects on murine leukemia virus integration in vivo and in vitro
Pryciak, P. M., & VARMUS, H. E. (1992) · Journal of Virology · doi:10.1128/jvi.66.10.5959-5966.1992 · Cited by 82
Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro
Bushman, F. D., & Craigie, R. (1990) · Journal of Virology · doi:10.1128/jvi.64.11.5645-5648.1990 · Cited by 81
Self-citation
Defining nucleic acid-binding properties of avian retrovirus integrase by deletion analysis
Mumm, S. R., & Grandgenett, D. P. (1991) · Journal of Virology · doi:10.1128/jvi.65.3.1160-1167.1991 · Cited by 81
Sequence of the circle junction of human immunodeficiency virus type 1: implications for reverse transcription and integration
Whitcomb, J. M., Kumar, R. V., & Hughes, S. H. (1990) · Journal of Virology · doi:10.1128/jvi.64.10.4903-4906.1990 · Cited by 80
Relationship of avian retrovirus DNA synthesis to integration in vitro.
Lee, Y. M., & Coffin, J. M. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.3.1419 · Cited by 77
Simian Immunodeficiency Virus Integration Preference Is Similar to That of Human Immunodeficiency Virus Type 1
Crise, B. J., Li, Y., Yuan, C. C., Morcock, D. R., Whitby, D., Munroe, D. J., Arthur, L. O., & Wu, X. (2005) · Journal of Virology · doi:10.1128/jvi.79.19.12199-12204.2005 · Cited by 73
A specific terminal structure is required for Ty1 transposition.Preprint
Eichinger, D. J., & Boeke, J. D. (1990) · Genes & Development · doi:10.1101/gad.4.3.324 · Cited by 73
Substrate features important for recognition and catalysis by human immunodeficiency virus type 1 integrase identified by using novel DNA substrates
Chow, S. A., & Brown, P. O. (1994) · Journal of Virology · doi:10.1128/jvi.68.6.3896-3907.1994 · Cited by 63
An integration-defective U5 deletion mutant of human immunodeficiency virus type 1 reverts by eliminating additional long terminal repeat sequences
Vicenzi, E., Dimitrov, D. S., Engelman, A., Migone, T., Purcell, D. F. J., Leonard, J. M., Englund, G., & Martin, M. A. (1994) · Journal of Virology · doi:10.1128/jvi.68.12.7879-7890.1994 · Cited by 62
Concerted integration of viral DNA termini by purified avian myeloblastosis virus integrase
Fitzgerald, M. L., Vora, A. C., Zeh, W. G., & Grandgenett, D. P. (1992) · Journal of Virology · doi:10.1128/jvi.66.11.6257-6263.1992 · Cited by 59
Efficient autointegration of avian retrovirus DNA in vitro
Lee, Y. M., & Coffin, J. M. (1990) · Journal of Virology · doi:10.1128/jvi.64.12.5958-5965.1990 · Cited by 59
Characterization of the forward and reverse integration reactions of the Moloney murine leukemia virus integrase protein purified from Escherichia coli.
Jonsson, C. B., Donzella, G. A., & Roth, M. J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54098-9 · Cited by 59
Removal of 3'-OH-terminal nucleotides from blunt-ended long terminal repeat termini by the avian retrovirus integration protein
Vora, A. C., Fitzgerald, M. L., & Grandgenett, D. P. (1990) · Journal of Virology · doi:10.1128/jvi.64.11.5656-5659.1990 · Cited by 58
Retroviral DNA Integration
Craigie, R. (2002) · doi:10.1128/9781555817954.ch25 · Cited by 24
Self-citation
Molecular mechanism of retroviral DNA integration
Kulkosky, J., & Skalka, A. M. (1994) · Pharmacology & Therapeutics · doi:10.1016/0163-7258(94)90062-0 · Cited by 56
Analysis of mutations in the integration function of Moloney murine leukemia virus: effects on DNA binding and cutting
Roth, M. J., Schwartzberg, P. L., Tanese, N., & Goff, S. P. (1990) · Journal of Virology · doi:10.1128/jvi.64.10.4709-4717.1990 · Cited by 53
Plus-strand origin for human immunodeficiency virus type 1: implications for integration
Pullen, K., & Champoux, J. J. (1990) · Journal of Virology · doi:10.1128/jvi.64.12.6274-6277.1990 · Cited by 50
Activities of the feline immunodeficiency virus integrase protein produced in Escherichia coli
Vink, C., Linden, K. H. V. D., & Plasterk, R. H. A. (1994) · Journal of Virology · doi:10.1128/jvi.68.3.1468-1474.1994 · Cited by 49
Modulation of Activity of Moloney Murine Leukemia Virus Preintegration Complexes by Host Factors In Vitro
Li, L., Farnet, C. M., Anderson, W. F., & Bushman, F. D. (1998) · Journal of Virology · doi:10.1128/jvi.72.3.2125-2131.1998 · Cited by 49
Endonucleolytic cleavages and DNA-joining activities of the integration protein of human foamy virus
Pahl, A., & Flügel, R. M. (1993) · Journal of Virology · doi:10.1128/jvi.67.9.5426-5434.1993 · Cited by 48
Role of the His-Cys finger of Moloney murine leukemia virus integrase protein in integration and disintegration
Jonsson, C. B., & Roth, M. J. (1993) · Journal of Virology · doi:10.1128/jvi.67.9.5562-5571.1993 · Cited by 48
The Roles of Cellular Factors in Retroviral Integration
Engelman, A. (2003) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-19012-4_6 · Cited by 45
Analysis of NCp7-dependent Activation of HIV-1 cDNA Integration and its Conservation Among Retroviral Nucleocapsid Proteins
Poljak, L. G., Batson, S. M., Ficheux, D., Roques, B. P., Darlix, J., & Käs, E. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00472-8 · Cited by 45
Retroviral integration: in vitro host site selection by avian integrase
Fitzgerald, M. L., & Grandgenett, D. P. (1994) · Journal of Virology · doi:10.1128/jvi.68.7.4314-4321.1994 · Cited by 42
Juxtaposition of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or murine leukemia virus integrase
Chow, S. A., & Brown, P. O. (1994) · Journal of Virology · doi:10.1128/jvi.68.12.7869-7878.1994 · Cited by 38
Characterization of Mg2+-Dependent 3'-Processing Activity for Human Immunodeficiency Virus Type 1 Integrase in Vitro: Real-Time Kinetic Studies Using Fluorescence Resonance Energy Transfer
Lee, S. P., Kim, H. G., Censullo, M. L., & Han, M. K. (1995) · Biochemistry · doi:10.1021/bi00032a014 · Cited by 37
Circular DNA of human immunodeficiency virus: analysis of circle junction nucleotide sequences
Hong, T., Drlica, K., Pinter, A., & Murphy, E. (1991) · Journal of Virology · doi:10.1128/jvi.65.1.551-555.1991 · Cited by 39
Characterization of recombinant murine leukemia virus integrase
Dotan, I., Scottoline, B. P., Heuer, T. S., & Brown, P. O. (1995) · Journal of Virology · doi:10.1128/jvi.69.1.456-468.1995 · Cited by 36
The istA gene of insertion sequence IS21 is essential for cleavage at the inner 3′ ends of tandemly repeated IS21 elements in vitro.
Reimmann, C., & Haas, D. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07627.x · Cited by 36
Viral gene products and replication of the human immunodeficiency type 1 virus
Morrow, C. D., Park, J., & Wakefield, J. (1994) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1994.266.5.c1135 · Cited by 34
Retroviral cDNA Integration: Mechanism, Applications and Inhibition
Hansen, M. S. T., Carteau, S., Hoffmann, C. J., Li, L., & Bushman, F. D. (1998) · Springer eBooks · doi:10.1007/978-1-4899-1739-3_3 · Cited by 33
Molecular mechanisms of nucleic acid integration
Coffin, J. M. (1990) · Journal of Medical Virology · doi:10.1002/jmv.1890310109 · Cited by 31
Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro
Yoshinaga, T., & Fujiwara, T. (1995) · Journal of Virology · doi:10.1128/jvi.69.5.3233-3236.1995 · Cited by 29
Self-citation
HIV-1 Replication and Potential Targets for Intervention
Stevenson, M., Bukrinsky, M., & Haggerty, S. (1992) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1992.8.107 · Cited by 29
Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions
Barsov, E. V., Huber, W., Marcotrigiano, J., Clark, P. K., Clark, A. D., Arnold, E., & Hughes, S. H. (1996) · Journal of Virology · doi:10.1128/jvi.70.7.4484-4494.1996 · Cited by 28
Isolation and Characterization of an Oligomerization-Negative Mutant of HIV-1 Integrase
Kalpana, G. V., Reicin, A. S., Cheng, G. S. W., Sorin, M., Paik, S., & Goff, S. P. (1999) · Virology · doi:10.1006/viro.1999.9767 · Cited by 27
Mutations in the U5 Sequences Adjacent to the Primer Binding Site Do Not Affect tRNA Cleavage by Rous Sarcoma Virus RNase H but Do Cause Aberrant Integrations In Vivo
Oh, J., Chang, K. W., & Hughes, S. H. (2005) · Journal of Virology · doi:10.1128/jvi.80.1.451-459.2006 · Cited by 26
Substrate Sequence Selection by Retroviral Integrase
Zhou, H., Rainey, G. J., Wong, S.-K., & Coffin, J. M. (2001) · Journal of Virology · doi:10.1128/jvi.75.3.1359-1370.2001 · Cited by 26
Fluorescent Nucleoside Analogues as DNA Probes
Hawkins, M. E. (2005) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-47947-8_5 · Cited by 25
Integration of human immunodeficiency virus types 1 and 2 DNA in vitro by cytoplasmic extracts of Moloney murine leukemia virus-infected mouse NIH 3T3 cells
Vink, C., van Gent, D. C., & Plasterk, R. H. A. (1990) · Journal of Virology · doi:10.1128/jvi.64.10.5219-5222.1990 · Cited by 23
Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein
Krogstad, P. A., & Champoux, J. J. (1990) · Journal of Virology · doi:10.1128/jvi.64.6.2796-2801.1990 · Cited by 23
In Vitro Integration of Retrotransposon Ty1: a Direct Physical Assay
Braiterman, L. T., & Boeke, J. D. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5719 · Cited by 22
Comparison of DNA binding and integration half-site selection by avian myeloblastosis virus integrase
Grandgenett, D. P., Inman, R. B., Vora, A. C., & Fitzgerald, M. L. (1993) · Journal of Virology · doi:10.1128/jvi.67.5.2628-2636.1993 · Cited by 22
The Multifaceted Contributions of Chromatin to HIV-1 Integration, Transcription, and Latency
Crignis, E. D., & Mahmoudi, T. (2016) · International review of cytology · doi:10.1016/bs.ircmb.2016.08.006 · Cited by 23
Substrate-Length-Dependent Activities of Human Immunodeficiency Virus Type 1 Integrase in Vitro: Differential DNA Binding Affinities Associated with Different Lengths of Substrates
Lee, S. P., Censullo, M. L., Kim, H. G., & Han, M. K. (1995) · Biochemistry · doi:10.1021/bi00032a015 · Cited by 21
Influence of substrate structure on disintegration activity of Moloney murine leukemia virus integrase
Donzella, G. A., Jonsson, C. B., & Roth, M. J. (1993) · Journal of Virology · doi:10.1128/jvi.67.12.7077-7087.1993 · Cited by 21
Ty1 In Vitro Integration: Effects of Mutations in cis and in trans
Braiterman, L. T., & Boeke, J. D. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5731 · Cited by 20
Host Proteins in Retroviral DNA Integration
Bushman, F. D. (1999) · Advances in virus research · doi:10.1016/s0065-3527(08)60303-6 · Cited by 20
Integration Site and Clonal Expansion in Human Chronic Retroviral Infection and Gene Therapy
Niederer, H. A., & Bangham, C. R. M. (2014) · Viruses · doi:10.3390/v6114140 · Cited by 23
Nucleoprotein Intermediates in HIV-1 DNA Integration: Structure and Function of HIV-1 Intasomes
Craigie, R. (2018) · Sub-cellular biochemistry/Subcellular biochemistry · doi:10.1007/978-981-10-8456-0_9 · Cited by 19
Self-citation
The sequence of human immunodeficiency virus type 2 circle junction suggests that integration protein cleaves the ends of linear DNA asymmetrically
Whitcomb, J. M., & Hughes, S. H. (1991) · Journal of Virology · doi:10.1128/jvi.65.7.3906-3910.1991 · Cited by 18
Detection and characterization of a functional complex of human immunodeficiency virus type 1 integrase and its DNA substrate by UV cross-linking
Yoshinaga, T., Kimura-Ohtani, Y., & Fujiwara, T. (1994) · Journal of Virology · doi:10.1128/jvi.68.9.5690-5697.1994 · Cited by 17
Self-citation
Molecular Biology of Lentivirus-Mediated Gene Transfer
Stevenson, M. (2002) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-56114-6_1 · Cited by 17
Assembly and Catalysis of Concerted Two-End Integration Events by Moloney Murine Leukemia Virus Integrase
Yang, F., & Roth, M. J. (2001) · Journal of Virology · doi:10.1128/jvi.75.20.9561-9570.2001 · Cited by 17
Reconstitution of Concerted DNA Integration with Purified Components
Hindmarsh, P. L., & Leis, J. P. (1999) · Advances in virus research · doi:10.1016/s0065-3527(08)60308-5 · Cited by 16
Subterminal viral DNA nucleotides as specific recognition signals for human immunodeficiency virus type 1 and visna virus integrases under magnesium-dependent conditions
Morgan, A. L., & Katzman, M. (2000) · Journal of General Virology · doi:10.1099/0022-1317-81-3-839 · Cited by 15
Rous Sarcoma Virus (RSV) Integration In Vivo: a CA Dinucleotide Is Not Required in U3, and RSV Linear DNA Does Not Autointegrate
Oh, J., Chang, K. W., Wierzchoslawski, R., Alvord, W. G., & Hughes, S. H. (2007) · Journal of Virology · doi:10.1128/jvi.01441-07 · Cited by 13
The Integrase: An Overview of a Key Player Enzyme in the Antiviral Scenario
Renzi, G., Carta, F., & Supuran, C. T. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241512187 · Cited by 11
Structural Insights on Retroviral DNA Integration: Learning from Foamy Viruses
Lee, G.-E., Mauro, E., Parissi, V., Shin, C., & Lesbats, P. (2019) · Viruses · doi:10.3390/v11090770 · Cited by 11
DNA Polymerases
Eun, H. (1996) · Elsevier eBooks · doi:10.1016/b978-012243740-3/50009-0 · Cited by 13
DNA Methylation and retrovirus expression
Bednarik, D. P. (1993) · Birkhäuser Basel eBooks · doi:10.1007/978-3-0348-9118-9_13 · Cited by 9
Effects of varying the spacing within the D,D-35-E motif in the catalytic region of retroviral integrase
Raup‐Konsavage, W. M., Sudol, M., & Katzman, M. (2008) · Virology · doi:10.1016/j.virol.2008.07.001 · Cited by 9
Moloney murine leukemia virus integration protein produced in yeast binds specifically to viral att sites
Basu, S., & VARMUS, H. E. (1990) · Journal of Virology · doi:10.1128/jvi.64.11.5617-5625.1990 · Cited by 9
Retracted
Ty1 In Vitro Integration: Effects of Mutations in cis and in trans
Braiterman, L. T., & Boeke, J. D. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5731-5740.1994 · Cited by 8
Moloney murine leukemia virus in protein from disrupted virions binds and specifically cleaves its target sequence in vitro
Ishimoto, L. K., Halperin, M., & Champouxi James, J. (1991) · Virology · doi:10.1016/0042-6822(91)90066-k · Cited by 8
Transgenic Animals
Brem, G. (1992) · Biotechnology · doi:10.1002/9783527620838.ch18 · Cited by 8
Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide Is Not Required for Integration of the U3 End of Viral DNA
Oh, J., Chang, K. W., & Hughes, S. H. (2008) · Journal of Virology · doi:10.1128/jvi.01353-08 · Cited by 7
Expression of HIV-1 Integrase in E. coli: Immunological Analysis of the Recombinant Protein
Marcus-Sekura, C. J., Woerner, A. M., Zweig, M., Court, D. L., Levin, J. G., & Klutch, M. J. (1990) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1990.6.1399 · Cited by 7

Authors: Fujiwara, T., & Craigie, R.
Affiliations: National Institute of Diabetes and Digestive and Kidney Diseases (US)
ORCID: Craigie, R
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1989-05
Volume: 86
Issue: 9
Pages: 3065-3069
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.86.9.3065
PMC: PMC287065
OpenAlex: W1971994381
Cited by: 109
References: 13
Citations by year: 2025: 1 · 2023: 2 · 2019: 1 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 1
FWCI: 9.02
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Keywords: retroviral integration, Moloney murine leukemia virus, reverse transcription, target site duplication, divalent cations
MeSH terms: Animals, Base Sequence, Cell Line, Cell-Free System, Chromatography, Gel, Cytoplasm, DNA Nucleotidyltransferases, DNA, Viral, Moloney murine leukemia virus, Bacteriophage lambda, Recombination, Genetic, Viral Proteins, Virus Replication, Integrases, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks