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Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor.
Open accessAbstract
Mutation frequency decline (MFD) is the rapid decrease in the frequency of certain induced nonsense suppressor mutations occurring when protein synthesis is transiently inhibited immediately after irradiation. MFD is abolished by mutations in the uvrA, -B, or -C genes, which prevent excision repair, or by a mfd mutation, which reduces the rate of excision but does not affect survival. Using an in vitro repair synthesis assay we found that although wild-type cells repair the transcribed (template) strand preferentially, mfd- cells are incapable of strand-specific repair. The deficiency in strand-selective repair of mfd- cell extract was corrected by adding highly purified "transcription-repair coupling factor" to the reaction mixture. We conclude that mfd is, most likely, the gene encoding the transcription-repair coupling factor.
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Metadata
- Authors
- Selby, C. P., Witkin, E. M., & Sancar, A.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- University of North Carolina at Chapel Hill (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1991-12-15
- Volume
- 88
- Issue
- 24
- Pages
- 11574-11578
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC53178
- OpenAlex
- W2153139649
- Cited by
- 215
- References
- 37
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 8 · 2015: 3 · 2014: 6 · 2013: 7 · 2012: 4
- FWCI
- 6.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM032833, GM32833)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Keywords
- strand-specific repair, excision repair, DNA repair, Escherichia coli
- MeSH terms
- Alleles, Bacterial Proteins, Cell-Free System, DNA Repair, Escherichia coli, Genes, Bacterial, Genetic Complementation Test, Mutation, Phenotype, Plasmids, SOS Response, Genetics, Transcription Factors, Transcription, Genetic, Ultraviolet Rays, Genes, Suppressor, Mutagenesis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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