Control of human muscle-type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor

Mascaró, Cristina; Acosta, Elena; Ortiz, José A.; Marrero, Pedro F.; Hegardt, Fausto G.; Haro, Diego

doi:10.1074/jbc.273.15.8560

Cited by 318Peroxisome Proliferator-Activated ReceptorsAdipose Tissue and MetabolismCancer, Lipids, and Metabolism

Control of human muscle-type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor

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Mascaró, Cristina, Acosta, Elena, Ortiz, José A., Marrero, Pedro F., Hegardt, Fausto G., Haro, Diego·1998·Journal of Biological Chemistry

DOI 10.1074/jbc.273.15.8560

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Abstract

The expression of several genes involved in intra- and extracellular lipid metabolism, notably those involved in peroxisomal and mitochondrial beta-oxidation, is mediated by ligand-activated receptors, collectively referred to as peroxisome proliferator-activated receptors (PPARs). To gain more insight into the control of expression of carnitine palmitoyltransferase (CPT) genes, which are regulated by fatty acids, we have examined the transcriptional regulation of the human MCPT I gene. We have cloned by polymerase chain reaction the 5'-flanking region of this gene and demonstrated its transcriptional activity by transfection experiments with the CAT gene as a reporter. We have also shown that this is a target gene for the action of PPARs, and we have localized a PPAR responsive element upstream of the first exon. These results show that PPAR regulates the entry of fatty acids into the mitochondria, which is a crucial step in their metabolism, especially in tissues like heart, skeletal muscle and brown adipose tissue in which fatty acids are a major source of energy.

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Sensing of free fatty acid availability within the cell primarily occurs through peroxisome proliferator-activated receptors, which function as fatty acid-activated transcription factors that drive the expression of CPT1.
“Sensing of free fatty acid availability within the cell primarily occurs through peroxisome proliferator–activated receptors, which function as fatty acid–activated transcription factors that drive the expression of CPT1 (64, 65).”
From Regulation and function of the mammalian tricarboxylic acid cycle (2023) · cites this paper as [65]
PPARα regulates peroxisomal and mitochondrial fatty acid β-oxidation.
“Since PPARα regulates peroxisomal and mitochondrial fatty acid β-oxidation [59]”
From Fibrates as drugs with senolytic and autophagic activity for osteoarthritis therapy (2019) · cites this paper as [59]

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Authors: Mascaró, C., Acosta, E., Ortiz, J. A., Marrero, P. F., Hegardt, F. G., & Haro, D.
Affiliations: Universitat de Barcelona (ES)
Corresponding author: Haro, Diego
ORCID: Mascaró, Cristina
Ortiz, José A.
Marrero, Pedro F.
Haro, Diego
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1998-04
Volume: 273
Issue: 15
Pages: 8560-8563
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1074/jbc.273.15.8560
OpenAlex: W2080318925
Cited by: 318
References: 26
Citations by year: 2025: 4 · 2024: 6 · 2023: 1 · 2022: 3 · 2021: 12 · 2020: 4 · 2019: 9 · 2018: 5 · 2017: 7 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 9 · 2012: 12
FWCI: 7.09
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 8227 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
PubMed (publishedVersion)
Funders: Universitat de Barcelona
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords: carnitine palmitoyltransferase 1, peroxisome proliferator-activated receptors, gene transcription, 5'-flanking region, PPAR-responsive element, fatty acid beta-oxidation, skeletal muscle, gene cloning
MeSH terms: Animals, Base Sequence, Carnitine O-Palmitoyltransferase, Cell Line, Exons, Humans, Isoenzymes, Promoter Regions, Genetic, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, Transcription Factors, Transcription, Genetic, Transfection, Chloramphenicol O-Acetyltransferase, Gene Expression Regulation, Enzymologic, Consensus Sequence, Sequence Alignment, Genes, Reporter, Receptors, Cytoplasmic and Nuclear, Muscle, Skeletal, Mice

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Control of human muscle-type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor

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