Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase
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A 1329-base pair clone isolated from a human placenta cDNA library contains a full-length 837-base pair coding region for a 31.9-kDa protein whose deduced primary structure exhibits high homology to consensus sequences found in other NMN adenylyltransferases. Northern blotting detected a major 3.1-kilobase mRNA transcript as well as a minor 4.1-kilobase transcript in all human tissues examined. In several cancer cell lines, lower levels of mRNA expression were clearly evident. The gene encoding the human enzyme was mapped to chromosome band 1p32-35. High efficiency bacterial expression yielded 1.5 mg of recombinant enzyme/liter of culture medium. The molecular and kinetic properties of recombinant human NMN adenylyltransferase provide new directions for investigating metabolic pathways involving this enzyme.
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Three mammalian homologs NMNAT1-3 have been identified, with NMNAT2 being the most labile of the three isoforms with a half-life of approximately 4 h.
“Three mammalian homologs, NMNAT1 -3, have been identified (24)(25)(26), with NMNAT2 (nicotinamide mononucleotide adenylyltransferase 2) being the most labile of the three isoforms with a half-life of ,4 h (27).”
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- Authors
- Emanuelli, M., Carnevali, F., Saccucci, F., Pierella, F., Amici, A., Raffaelli, N., & Magni, G.
- Corresponding author
- Magni, Giulio
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 276
- Issue
- 1
- Pages
- 406-412
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2098215193
- Cited by
- 189
- References
- 36
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 1 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 6 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 12 · 2013: 8 · 2012: 15
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Università Politecnica delle Marche (Università Politecnica delle Marche) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
- Keywords
- NMN adenylyltransferase, nicotinamide mononucleotide, enzyme cloning, chromosomal localization, tissue mRNA expression, cancer cell lines, recombinant enzyme expression, enzyme kinetics, NAD biosynthesis
- MeSH terms
- Amino Acid Sequence, Base Sequence, Catalysis, Cations, Divalent, Chromosomes, Human, Pair 1, Cloning, Molecular, Escherichia coli, Humans, Kinetics, Molecular Sequence Data, Nicotinamide-Nucleotide Adenylyltransferase, Recombinant Proteins, RNA, Messenger, Tumor Cells, Cultured, Blotting, Southern, Sequence Alignment, In Situ Hybridization, Fluorescence, Gene Dosage, Physical Chromosome Mapping, Gene Expression Profiling
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