Cited by 189Biochemical and Molecular ResearchSirtuins and Resveratrol in MedicineFolate and B Vitamins Research

Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase

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Emanuelli, Monica, Carnevali, Francesco, Saccucci, Franca, Pierella, Francesca, Amici, Adolfo, Raffaelli, Nadia, Magni, Giulio·2001·Journal of Biological Chemistry

Abstract

A 1329-base pair clone isolated from a human placenta cDNA library contains a full-length 837-base pair coding region for a 31.9-kDa protein whose deduced primary structure exhibits high homology to consensus sequences found in other NMN adenylyltransferases. Northern blotting detected a major 3.1-kilobase mRNA transcript as well as a minor 4.1-kilobase transcript in all human tissues examined. In several cancer cell lines, lower levels of mRNA expression were clearly evident. The gene encoding the human enzyme was mapped to chromosome band 1p32-35. High efficiency bacterial expression yielded 1.5 mg of recombinant enzyme/liter of culture medium. The molecular and kinetic properties of recombinant human NMN adenylyltransferase provide new directions for investigating metabolic pathways involving this enzyme.

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  1. Cherney, B. W., McBride, O. W., Chen, D. F., Alkhatib, H., Bhatia, K., Hensley, P., and Smulson, M. E. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 8370 -8374
  2. Raffaelli, N., Lorenzi, T., Emanuelli, M., Amici, A., Ruggieri, S., and Magni, G. (2001) Methods Enzymol. 331, in press

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Authors
Emanuelli, M., Carnevali, F., Saccucci, F., Pierella, F., Amici, A., Raffaelli, N., & Magni, G.
Corresponding author
Magni, Giulio
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-01
Volume
276
Issue
1
Pages
406-412
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2098215193
Cited by
189
References
36
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 1 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 6 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 12 · 2013: 8 · 2012: 15
FWCI
4.05
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Università Politecnica delle Marche (Università Politecnica delle Marche) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
Keywords
NMN adenylyltransferase, nicotinamide mononucleotide, enzyme cloning, chromosomal localization, tissue mRNA expression, cancer cell lines, recombinant enzyme expression, enzyme kinetics, NAD biosynthesis
MeSH terms
Amino Acid Sequence, Base Sequence, Catalysis, Cations, Divalent, Chromosomes, Human, Pair 1, Cloning, Molecular, Escherichia coli, Humans, Kinetics, Molecular Sequence Data, Nicotinamide-Nucleotide Adenylyltransferase, Recombinant Proteins, RNA, Messenger, Tumor Cells, Cultured, Blotting, Southern, Sequence Alignment, In Situ Hybridization, Fluorescence, Gene Dosage, Physical Chromosome Mapping, Gene Expression Profiling

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