Cited by 137Sirtuins and Resveratrol in MedicineBiochemical and Molecular ResearchCalcium signaling and nucleotide metabolism

Location, Location, Location: Compartmentalization of NAD+ Synthesis and Functions in Mammalian Cells

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Cambronne, Xiaolu A., Kraus, W. Lee·2020·Trends in Biochemical Sciences

Abstract

The numerous biological roles of NAD+ are organized and coordinated via its compartmentalization within cells. The spatial and temporal partitioning of this intermediary metabolite is intrinsic to understanding the impact of NAD+ on cellular signaling and metabolism. We review evidence supporting the compartmentalization of steady-state NAD+ levels in cells, as well as how the modulation of NAD+ synthesis dynamically regulates signaling by controlling subcellular NAD+ concentrations. We further discuss potential benefits to the cell of compartmentalizing NAD+, and methods for measuring subcellular NAD+ levels.

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Crossref · 10.1016/j.tibs.2020.05.010

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  • NAD+ is highly compartmentalized in the cytoplasm, mitochondria and nucleus, which are its main subcellular pools.

    “NAD+ is highly compartmentalized in the cytoplasm, mitochondria and nucleus, which represent its main subcellular pools (Supplementary Box 1).”
  • The enzymes involved in NAD+ biosynthesis or degradation are highly compartmentalized.

    “These pools are regulated independently of each other, and consistent with this, the enzymes involved in the biosynthesis or degradation of NAD+ are highly compartmentalized as well2–4.”

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  1. [59]Nikiforov A (2011) Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation. J. Biol. Chem 286, 21767– 21778 21504897 PMC3122232

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Authors
Cambronne, X. A., & Kraus, W. L.
Affiliations
  • The University of Texas at Austin (US)
  • The University of Texas Southwestern Medical Center (US)
Corresponding author
  • Cambronne, Xiaolu A.
  • Kraus, W. Lee
Venue
Trends in Biochemical Sciences
Publisher
Elsevier BV
Published
2020-10
Volume
45
Issue
10
Pages
858-873
ISSN
0968-0004 (ISSN-L)
OpenAlex
W3037844642
Cited by
137
References
130
Citations by year
2026: 25 · 2025: 23 · 2024: 26 · 2023: 25 · 2022: 21 · 2021: 15 · 2020: 2
FWCI
1.95
Type
journal article
Language
en
Open access
Locations
  • Trends in Biochemical Sciences (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institutes of Health
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
NAD+, compartmentalization, NAD+ synthesis, cellular metabolism, signal transduction, subcellular compartmentalization
MeSH terms
Animals, Cell Compartmentation, NAD, Subcellular Fractions, Signal Transduction

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